Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,648	A→G	intergenic (‑2991/‑69570)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,648	0	A	G	100.0%	103.7 / NA	39	intergenic (‑2991/‑69570)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/14);  total (25/14)

GTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTA  >  1/852410‑852890                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    gTATAATATTACCGTTTCTTTCTTTTGTAGGCTGCCTAAACTTACCATCTAAAAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATAAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCAc                                                                                                                                                                                                                                           <  1:485284/249‑1 (MQ=255) gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                                                                                         >  1:606470/1‑251 (MQ=255) gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                                                                                         >  1:482367/1‑251 (MQ=255) gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                                                                                         >  1:332321/1‑251 (MQ=255) gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                                                                                         >  1:303701/1‑251 (MQ=255)      atattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCAc                                                                                                                                                                                                                                           >  1:120730/4‑244 (MQ=255)      atattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGt                                                                                                                                                                                                                                    >  1:8590/4‑251 (MQ=255)       tattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAAAAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGtt                                                                                                                                                                                                                                   <  1:560789/249‑2 (MQ=255)               tttctgtCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGttatggctaa                                                                                                                                                                                                                           >  1:602452/7‑242 (MQ=255)                        tttgtagGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGttatggctaaatcccaata                                                                                                                                                                                                                  >  1:303148/4‑233 (MQ=255)                              ggctgccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGt                                                                                                                                                                                                            >  1:792738/7‑251 (MQ=255)                                  gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGtgg                                                                                                                                                                                                          <  1:373668/247‑3 (MQ=255)                                  gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTa                                                                                                                                                                                                        >  1:481311/3‑251 (MQ=255)                                     tAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATa                                                                                                                                                                                                     <  1:731563/251‑1 (MQ=255)                                        acttACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGat                                                                                                                                                                                                  <  1:696224/248‑1 (MQ=255)                                                   aaTAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGat                                                                                                                                                                                                  >  1:768307/1‑240 (MQ=255)                                                         gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGtt                                                                                                                                                                                                                                   >  1:75084/4‑200 (MQ=255)                                                         gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGa                                                                                                                                                                                                   >  1:140087/4‑233 (MQ=255)                                                         gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAg                                                                                                                                                                                 >  1:360761/4‑251 (MQ=255)                                                                  caAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGaa                                                                                                                                                                        >  1:620439/1‑251 (MQ=255)                                                                          ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGttatggctaaatcccaatat                                                                                                                                                                                                                 >  1:233803/1‑183 (MQ=255)                                                                          ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                                                                                                >  1:726941/1‑248 (MQ=255)                                                                          ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                                                                                                >  1:690141/1‑248 (MQ=255)                                                                                aCCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGat                                                                                                                                                                  >  1:292311/1‑242 (MQ=255)                                                                                    caATCAACATATTTAACATCATTTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAg                                                                                                                                                                                                    <  1:764995/205‑1 (MQ=255)                                                                                                       caatttcatAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCaaa                                                                                                                                    >  1:274106/9‑250 (MQ=255)                                                                                                                                 aaaCGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                                                                                         <  1:111173/251‑1 (MQ=255)                                                                                                                                               gccTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAAttttt                                                                                           >  1:335046/2‑251 (MQ=255)                                                                                                                                                   ataGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                                                                                         >  1:371139/3‑233 (MQ=255)                                                                                                                                                                    ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                      <  1:138808/251‑3 (MQ=255)                                                                                                                                                                    ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                      <  1:304702/251‑3 (MQ=255)                                                                                                                                                                     tAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                     <  1:596994/251‑4 (MQ=255)                                                                                                                                                                                 tgttTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGAtattac                                                       <  1:179337/251‑2 (MQ=255)                                                                                                                                                                                             ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                     <  1:570457/224‑4 (MQ=255)                                                                                                                                                                                             ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                      <  1:663824/223‑3 (MQ=255)                                                                                                                                                                                             ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGtt                                           >  1:423956/4‑251 (MQ=255)                                                                                                                                                                                                                 atcgttaccttccgccgTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCatta                       >  1:482743/17‑251 (MQ=255)                                                                                                                                                                                                                                  ttCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAgggga      <  1:35572/251‑4 (MQ=255)                                                                                                                                                                                                                                      gtttttAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGtg  >  1:494047/2‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    GTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTA  >  1/852410‑852890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑