Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,578	C→T	intergenic (‑2921/‑69640)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,578	0	C	T	100.0%	135.3 / NA	50	intergenic (‑2921/‑69640)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/9);  total (41/9)

AAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCA‑TTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTA  >  1/852333‑852823                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aaaTGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAgt                                                                                                                                                                                                                                                    >  1:160468/1‑250 (MQ=255)                                 aGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAAt                                                                                                                                                                                                                            >  1:724858/1‑242 (MQ=255)                                   gttaaTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATa                                                                                                                                                                                                                 >  1:841116/4‑251 (MQ=255)                                      aaTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAAt                                                                                                                                                                                                               >  1:784424/1‑250 (MQ=255)                                      aaTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGAGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAAt                                                                                                                                                                                                               >  1:634449/1‑250 (MQ=255)                                       aTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATcg                                                                                                                                                                                                             >  1:399960/1‑250 (MQ=255)                                       aTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATcg                                                                                                                                                                                                             >  1:480908/1‑250 (MQ=255)                                        tGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTc                                                                                                                                                                                                                          >  1:247496/1‑237 (MQ=255)                                                cAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAccttc                                                                                                                                                                                                     >  1:110985/1‑247 (MQ=255)                                                cAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAccttc                                                                                                                                                                                                     >  1:640058/1‑247 (MQ=255)                                                        gTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAccttccgccgtt                                                                                                                                                                                              >  1:323401/1‑239 (MQ=255)                                                               acctaCCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTa                                                                                                                                                                                     >  1:151568/5‑251 (MQ=255)                                                                   aCCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGcga                                                                                                                                                                                 >  1:223428/1‑249 (MQ=255)                                                                    ccA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTag                                                                                                                                                                                                                                         >  1:507792/1‑194 (MQ=255)                                                                    ccA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGcgat                                                                                                                                                                                >  1:237468/1‑248 (MQ=255)                                                                               gTATAATATTACCGTTTCTTTCTTTTGTAGGCTGCCTAAACTTACCATCTAAAAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATAAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCAc                                                                                                                                                                        <  1:485284/249‑1 (MQ=255)                                                                               gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                      >  1:303701/1‑251 (MQ=255)                                                                               gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                      >  1:482367/1‑251 (MQ=255)                                                                               gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                      >  1:332321/1‑251 (MQ=255)                                                                               gTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACtt                                                                                                                                                                      >  1:606470/1‑251 (MQ=255)                                                                                    atattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTc                                                                                                                                                                                                                          >  1:168314/4‑194 (MQ=255)                                                                                    atattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCAc                                                                                                                                                                        >  1:120730/4‑244 (MQ=255)                                                                                    atattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGt                                                                                                                                                                 >  1:8590/4‑251 (MQ=255)                                                                                     tattACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAAAAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGtt                                                                                                                                                                <  1:560789/249‑2 (MQ=255)                                                                                             tttctgtCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGttatggctaa                                                                                                                                                        >  1:602452/7‑242 (MQ=255)                                                                                                      tttgtagGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGttatggctaaatcccaata                                                                                                                                               >  1:303148/4‑233 (MQ=255)                                                                                                            ggctgccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGt                                                                                                                                         >  1:792738/7‑251 (MQ=255)                                                                                                                gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAcc                                                                                                                                                                                                        <  1:49506/182‑1 (MQ=255)                                                                                                                gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGtgg                                                                                                                                       <  1:373668/247‑3 (MQ=255)                                                                                                                gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTa                                                                                                                                     >  1:481311/3‑251 (MQ=255)                                                                                                                   tAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATa                                                                                                                                  <  1:731563/251‑1 (MQ=255)                                                                                                                      acttACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGat                                                                                                                               <  1:696224/248‑1 (MQ=255)                                                                                                                                 aaTAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGat                                                                                                                               >  1:768307/1‑240 (MQ=255)                                                                                                                                       gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAAt                                                                                                                                                                                                               >  1:770977/4‑154 (MQ=255)                                                                                                                                       gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGtt                                                                                                                                                                >  1:75084/4‑200 (MQ=255)                                                                                                                                       gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGa                                                                                                                                >  1:140087/4‑233 (MQ=255)                                                                                                                                       gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAg                                                                                                              >  1:360761/4‑251 (MQ=255)                                                                                                                                           taatacaAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGttt                                                                                                                                                                                        >  1:348243/5‑173 (MQ=255)                                                                                                                                                caAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGaa                                                                                                     >  1:620439/1‑251 (MQ=255)                                                                                                                                                        ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGttatggctaaatcccaatat                                                                                                                                              >  1:233803/1‑183 (MQ=255)                                                                                                                                                        ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                             >  1:690141/1‑248 (MQ=255)                                                                                                                                                        ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                             >  1:726941/1‑248 (MQ=255)                                                                                                                                                          cACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGc                                                                                                                                                                                   >  1:684998/1‑163 (MQ=255)                                                                                                                                                              aCCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGat                                                                                               >  1:292311/1‑242 (MQ=255)                                                                                                                                                                  caATCAACATATTTAACATCATTTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAg                                                                                                                                 <  1:764995/205‑1 (MQ=255)                                                                                                                                                                                     caatttcatAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCaaa                                                                 >  1:274106/9‑250 (MQ=255)                                                                                                                                                                                                               aaaCGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                      <  1:111173/251‑1 (MQ=255)                                                                                                                                                                                                                             gccTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAAttttt                        >  1:335046/2‑251 (MQ=255)                                                                                                                                                                                                                                 ataGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                      >  1:371139/3‑233 (MQ=255)                                                                                                                                                                                                                                                  ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat   <  1:304702/251‑3 (MQ=255)                                                                                                                                                                                                                                                  ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat   <  1:138808/251‑3 (MQ=255)                                                                                                                                                                                                                                                   tAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc  <  1:596994/251‑4 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCA‑TTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTA  >  1/852333‑852823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑