Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	111,719	G→A	intergenic (–/+19415)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	111,719	0	G	A	100.0%	237.2 / NA	87	intergenic (–/+19415)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/50);  total (37/50)

CCTCAAAACTGTTATTAGAAATAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGGATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTAGATTTGAATTACTATTTATTGAAGTTTTTGATACATCACCTTTTTCTGAAACTGAAAATACAGCGCCAGTATTA  >  1/111475‑111962                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ccTCAAAACTGTTATTAGAAATAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGC‑CACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATAtttt                                                                                                                                                                                                                                              >  1:831166/1‑251 (MQ=255)      aaaCTGTTATTAGAAATAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAg                                                                                                                                                                                                                                           >  1:276835/1‑250 (MQ=255)                   aaaTAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCAt                                                                                                                                                                                                                               <  1:586772/249‑1 (MQ=255)                   aaaTAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTg                                                                                                                                                                                                                             >  1:129920/1‑251 (MQ=255)                      tAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTaa                                                                                                                                                                                                                          >  1:847152/1‑251 (MQ=255)                        gTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATAttt                                                                                                                                                                                                                                               >  1:618069/1‑228 (MQ=255)                        gTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTaaaa                                                                                                                                                                                                                        >  1:297644/1‑251 (MQ=255)                         ttGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAAt                                                                                                                                                                                                                       <  1:414932/251‑1 (MQ=255)                            cATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATAc                                                                                                                                                                                                                     <  1:120239/250‑1 (MQ=255)                            cATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATAc                                                                                                                                                                                                                     <  1:198451/250‑1 (MQ=255)                                cATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATAc                                                                                                                                                                                                                     >  1:876876/1‑246 (MQ=255)                                cATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGAt                                                                                                                                                                                                                >  1:184005/1‑251 (MQ=255)                                  tatTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGt                                                                                                                                                                                                                            <  1:897996/237‑1 (MQ=255)                                           cTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGaaa                                                                                                                                                                                                     >  1:345600/1‑251 (MQ=255)                                               agaATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAAtct                                                                                                                                                                                                  <  1:399367/250‑1 (MQ=255)                                                   tCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGGATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAAtctt                                                                                                                                                                                                 >  1:435446/1‑247 (MQ=255)                                                   tCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAAtctt                                                                                                                                                                                                 >  1:341492/1‑247 (MQ=255)                                                    cAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATAttt                                                                                                                                                                                                                                               <  1:809873/200‑1 (MQ=255)                                                        ggACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCAt                                                                                                                                                                                                                               >  1:712967/1‑212 (MQ=255)                                                          aCATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaa                                                                                                                                                                                      <  1:11698/251‑1 (MQ=255)                                                           cATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaag                                                                                                                                                                                     <  1:291071/251‑1 (MQ=255)                                                            atatTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaaga                                                                                                                                                                                    >  1:158506/1‑251 (MQ=255)                                                             tatTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTaaaa                                                                                                                                                                                                                        >  1:673086/1‑214 (MQ=255)                                                               tttttACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTaa                                                                                                                                                                                                                          >  1:459821/1‑210 (MQ=255)                                                                    aCGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAAc                                                                                                                                                                            <  1:257856/251‑1 (MQ=255)                                                                     cGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAAcc                                                                                                                                                                           <  1:492828/251‑1 (MQ=255)                                                                     cGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAAcc                                                                                                                                                                           <  1:326479/251‑1 (MQ=255)                                                                     cGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAAcc                                                                                                                                                                           <  1:333556/251‑1 (MQ=255)                                                                      gTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTtctc                                                                                                                                                                                                                                  <  1:702201/195‑1 (MQ=255)                                                                      gTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTaga                                                                                                                                                                                       <  1:339010/238‑1 (MQ=255)                                                                       ttCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATAttt                                                                                                                                                                                                                                               <  1:830694/181‑1 (MQ=255)                                                                         cTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTaa                                                                                                                                                                                                                          >  1:406206/1‑200 (MQ=255)                                                                         cTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaa                                                                                                                                                                                      >  1:479154/1‑236 (MQ=255)                                                                         cTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCatat                                                                                                                                                                       >  1:613123/1‑251 (MQ=255)                                                                         cTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCatat                                                                                                                                                                       >  1:288136/1‑251 (MQ=255)                                                                         cTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCatat                                                                                                                                                                       <  1:604701/251‑1 (MQ=255)                                                                                          aaGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATAc                                                                                                                                                                                                                     >  1:881687/1‑188 (MQ=255)                                                                                          aaGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAAtcttc                                                                                                                                                                                                <  1:669038/209‑1 (MQ=255)                                                                                             aTAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTtc                                                                                                                                                     <  1:69015/249‑1 (MQ=255)                                                                                                       cATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAAcc                                                                                                                                                                           <  1:516752/217‑1 (MQ=255)                                                                                                        aTCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaac                                                                                                                                        <  1:353610/251‑1 (MQ=255)                                                                                                        aTCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaac                                                                                                                                        <  1:251467/251‑1 (MQ=255)                                                                                                        aTCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaac                                                                                                                                        <  1:349819/251‑1 (MQ=255)                                                                                                         tCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAggg                                                                                                                                                                                                        <  1:828192/186‑1 (MQ=255)                                                                                                                tttttGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaactataacta                                                                                                                                <  1:16866/251‑1 (MQ=255)                                                                                                                       cccAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGtt                                                                                                                                                      <  1:386961/222‑1 (MQ=255)                                                                                                                          aaaaTCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaag                                                                                                                                                                                     >  1:771891/1‑188 (MQ=255)                                                                                                                            aaTCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCa                                                                                                                    <  1:511852/251‑1 (MQ=255)                                                                                                                                 gCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGt                                                                                                                 >  1:502560/1‑249 (MQ=255)                                                                                                                                  ctctAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGt                                                                                                                 <  1:799191/248‑1 (MQ=255)                                                                                                                                  ctctAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTa                                                                                                                <  1:654694/249‑1 (MQ=255)                                                                                                                                      aaTTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATc                                                                                                                                                                                                               <  1:172927/150‑1 (MQ=255)                                                                                                                                           cTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAACATACTTGATCATTAGGGAAATCTTCTGTCTTAGACGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaac                                                                                                                                        >  1:454095/1‑216 (MQ=255)                                                                                                                                              cTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGt                                                                                                  <  1:48645/251‑1 (MQ=255)                                                                                                                                                     ttttGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCaa                                                                                           <  1:114054/251‑1 (MQ=255)                                                                                                                                                      tttGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAat                                                                                          >  1:507529/1‑251 (MQ=255)                                                                                                                                                        tGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAatat                                                                                        <  1:81964/251‑1 (MQ=255)                                                                                                                                                         gTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAatat                                                                                        <  1:814377/250‑1 (MQ=255)                                                                                                                                                          ttAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCt                                                                                      <  1:629174/251‑1 (MQ=255)                                                                                                                                                                gCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGAt                                                                                                                                                                                                                >  1:781807/1‑123 (MQ=255)                                                                                                                                                                   cACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATAc                                                                                                                                                                                                                     <  1:779102/115‑1 (MQ=255)                                                                                                                                                                   cACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaactataac                                                                                                                                  >  1:842428/1‑198 (MQ=255)                                                                                                                                                                         cTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATAc                                                                                                                                                                                                                     >  1:438902/1‑109 (MQ=255)                                                                                                                                                                         cTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAAcc                                                                                                                                                                           <  1:747549/151‑1 (MQ=255)                                                                                                                                                                           ccGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaag                                                                     >  1:128506‑M1/1‑243 (MQ=255)                                                                                                                                                                                          tCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTcc                                                                                                                                                                                                                                 <  1:464820/80‑1 (MQ=255)                                                                                                                                                                                          tCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCatat                                                                                                                                                                       >  1:414741/1‑138 (MQ=255)                                                                                                                                                                                          tCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGc                                                                                                                                                             >  1:24499/1‑148 (MQ=255)                                                                                                                                                                                                 tctGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCa                                                                                                                    <  1:768958/182‑1 (MQ=255)                                                                                                                                                                                                        accTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttctgaaa                                          >  1:876879‑M1/1‑214 (MQ=255)                                                                                                                                                                                                         ccTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaa                                                                                                                                                                                      <  1:106367/108‑1 (MQ=255)                                                                                                                                                                                                         ccTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaac                                                                                                                                        <  1:25693/154‑1 (MQ=255)                                                                                                                                                                                                         ccTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacc                                                     <  1:418958‑M1/237‑25 (MQ=255)                                                                                                                                                                                                          cTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAggg                                                                                                                                                                                                        <  1:81017/89‑1 (MQ=255)                                                                                                                                                                                                             taACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTagaa                                                                                                                                                                                      >  1:778834/1‑104 (MQ=255)                                                                                                                                                                                                             taACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttctgaaactgaaaa                                   >  1:11404‑M1/1‑209 (MQ=255)                                                                                                                                                                                                             taACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttc                                               >  1:192363‑M1/1‑209 (MQ=255)                                                                                                                                                                                                              aaCTACACTACCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGAt                                                                                                                                                                                                                <  1:770305/77‑1 (MQ=255)                                                                                                                                                                                                                     ctacCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGc                                                                                                                                                             <  1:97450/121‑1 (MQ=255)                                                                                                                                                                                                                     ctacCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCt                                                                                      <  1:468293/192‑1 (MQ=255)                                                                                                                                                                                                                     ctacCGGAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttctgaaactgaaaatacagcg                            >  1:440479‑M1/1‑201 (MQ=255)                                                                                                                                                                                                                           gAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGAt                                                                                                                                                                                                                >  1:123671/1‑64 (MQ=255)                                                                                                                                                                                                                           gAATAGCGGACTTCACCCGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGt                                                                                                                                                       <  1:213735/121‑1 (MQ=255)                                                                                                                                                                                                                                            cGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAaactataact                                                                                                                                 <  1:52992/126‑1 (MQ=255)                                                                                                                                                                                                                                            cGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcgga    <  1:700716‑M1/251‑74 (MQ=255)                                                                                                                                                                                                                                            cGCATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcgga    <  1:580482‑M1/251‑74 (MQ=255)                                                                                                                                                                                                                                              cATTAGGAATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTtattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagt  >  1:250570‑M1/1‑176 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CCTCAAAACTGTTATTAGAAATAGTTGCATTCATATTAATTACTTGAGAATCAGCGGACATATTTTTACGTTCTAAGGTTCCTGGCCCAAAGATAAATCGACCATCTAATTTTTTTGACCCAAAATCAGCTCTAATTTCTGCTTTACCTTTTGTTAATTGCTCACTAGCTCCGATAGGGGCGCCATCACTCTTCTGACCACCTATAACTACACTACCGGAATAGCGGACTTCACCCGCATTAGGGATATTTTTAGCAATTTCTCCATTGTAAAATACTTGATCATTAGGGAAATCTTCTGTCTTAGAAGATAAATAACCATATTGATAAAGGCTATTGTTCTCGCCTATAAAACTATAACTATCAGAATCTTCAAGTAGATTAATTTTATGTCCAGCAATATCTAACGCTTTTAGATTTGAATTACTATTTATTGAAGTTTTTGATACATCACCTTTTTCTGAAACTGAAAATACAGCGCCAGTATTA  >  1/111475‑111962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑