Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,398	G→T	intergenic (‑2741/‑69820)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,398	0	G	T	100.0%	81.0 / NA	31	intergenic (‑2741/‑69820)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/7);  total (24/7)

AAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCA‑TTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTT  >  1/852220‑852636                                                                                                                                                                                   |                                                                                                                                                                                                                                                tcatgaattaattattTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAAc                                                                                                                                                                                            >  1:768933/11‑231 (MQ=255)                 tgtttGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCttttgt                                                                                                                                                                                                         >  1:502462/3‑199 (MQ=255)                        cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                                                                                                                                                 <  1:355380/235‑1 (MQ=255)                        cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                                                                                                                                                 <  1:38067/235‑1 (MQ=255)                         ctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcaca                                                                                                                                                >  1:462618/16‑251 (MQ=255)                                         aTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGc                                                                                                                                                                                                     >  1:129261/1‑182 (MQ=255)                                                   aTTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAAGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat                                                                                                                      >  1:424603/1‑246 (MQ=255)                                                         ttcttGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcataaccg                                                                                                                 >  1:164474/5‑240 (MQ=255)                                                                          aCTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCAttt                                                                                                           >  1:392231/1‑239 (MQ=255)                                                                                 taacaacaacctgcaaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAATTTCATACTTGTAGGTTAATGAATACCCAGCCAATTTTGCTAACCTACCA‑TTGGGGGTTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAACCTTCCCATCTAATAAAAATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGAt                                                                                        <  1:649348/231‑1 (MQ=255)                                                                                           ctgctaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTat                                                                                 >  1:483397/11‑247 (MQ=255)                                                                                             gctaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAAc                                                                                                                                 >  1:446746/9‑198 (MQ=255)                                                                                                aacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATCCACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAACCTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCATTTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGagg                                                                         <  1:162447/246‑3 (MQ=255)                                                                                                  cgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTa                                                                                                                                   <  1:839709/188‑1 (MQ=255)                                                                                                  cgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat                                                                                                                      <  1:875529/201‑6 (MQ=255)                                                                                                        ccctAGGTAAAAATGGCAGCAAAACAAAAAATTAATACTTGTAGGTTAATAAATACACAGCCAATGTTGCTAACCTCCCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTCCCACAATCAACATATTAAACATCAATTTAATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGa                                                                 <  1:492464/248‑1 (MQ=255)                                                                                                              gTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGt                                                                              >  1:163409/1‑232 (MQ=255)                                                                                                                  aaaTGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAgt                                                        >  1:160468/1‑250 (MQ=255)                                                                                                                                                  aGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGaggt                                                                        >  1:854501/1‑199 (MQ=255)                                                                                                                                                  aGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAAt                                >  1:724858/1‑242 (MQ=255)                                                                                                                                                    gttaaTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATa                     >  1:841116/4‑251 (MQ=255)                                                                                                                                                       aaTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAAt                   >  1:784424/1‑250 (MQ=255)                                                                                                                                                       aaTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGAGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAAt                   >  1:634449/1‑250 (MQ=255)                                                                                                                                                        aTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATcg                 >  1:480908/1‑250 (MQ=255)                                                                                                                                                        aTGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATcg                 >  1:399960/1‑250 (MQ=255)                                                                                                                                                         tGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTg                                                                  >  1:871881/1‑201 (MQ=255)                                                                                                                                                         tGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTc                              >  1:247496/1‑237 (MQ=255)                                                                                                                                                                 cAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAccttc         >  1:640058/1‑247 (MQ=255)                                                                                                                                                                 cAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAccttc         >  1:110985/1‑247 (MQ=255)                                                                                                                                                                         gTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcataa                                                                                                                    >  1:872591/1‑128 (MQ=255)                                                                                                                                                                         gTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTAccttccgccgtt  >  1:323401/1‑239 (MQ=255)                                                                                                                                                                                   |                                                                                                                                                                                                                                                AAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCA‑TTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTT  >  1/852220‑852636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑