Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,350	G→A	intergenic (‑2693/‑69868)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,350	0	G	A	100.0%	45.9 / NA	19	intergenic (‑2693/‑69868)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/7);  total (12/7)

AAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCA‑TTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGC  >  1/852220‑852582                                                                                                                                   |                                                                                                                                                                                                                                          tcatgaattaattattTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAAc                                                                                                                                      >  1:768933/11‑231 (MQ=255)             tatttgttTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGaa                                                                                                                                                                                                                  >  1:78544/1‑144 (MQ=255)                 tgtttGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCttttgt                                                                                                                                                   >  1:502462/3‑199 (MQ=255)                        cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                                                                                           <  1:355380/235‑1 (MQ=255)                        cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                                                                                           <  1:38067/235‑1 (MQ=255)                         ctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcaca                                                                                          >  1:462618/16‑251 (MQ=255)                                         aTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGc                                                                                                                                               >  1:129261/1‑182 (MQ=255)                                                   aTTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAAGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat                                                                >  1:424603/1‑246 (MQ=255)                                                         ttcttGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcataaccg                                                           >  1:164474/5‑240 (MQ=255)                                                                          aCTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCAttt                                                     >  1:392231/1‑239 (MQ=255)                                                                                 taacaacaacctgcaaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAATTTCATACTTGTAGGTTAATGAATACCCAGCCAATTTTGCTAACCTACCA‑TTGGGGGTTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAACCTTCCCATCTAATAAAAATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGAt                                  <  1:649348/231‑1 (MQ=255)                                                                                           ctgctaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTat                           >  1:483397/11‑247 (MQ=255)                                                                                             gctaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAAc                                                                           >  1:446746/9‑198 (MQ=255)                                                                                                aacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATCCACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAACCTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCATTTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGagg                   <  1:162447/246‑3 (MQ=255)                                                                                                  cgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTa                                                                             <  1:839709/188‑1 (MQ=255)                                                                                                  cgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat                                                                <  1:875529/201‑6 (MQ=255)                                                                                                        ccctAGGTAAAAATGGCAGCAAAACAAAAAATTAATACTTGTAGGTTAATAAATACACAGCCAATGTTGCTAACCTCCCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTCCCACAATCAACATATTAAACATCAATTTAATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGa           <  1:492464/248‑1 (MQ=255)                                                                                                              gTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCA‑TTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGt                        >  1:163409/1‑232 (MQ=255)                                                                                                                  aaaTGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAgt  >  1:160468/1‑250 (MQ=255)                                                                                                                                   |                                                                                                                                                                                                                                          AAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCA‑TTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGC  >  1/852220‑852582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑