Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,325	T→C	intergenic (‑2668/‑69893)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,325	0	T	C	100.0%	41.9 / NA	17	intergenic (‑2668/‑69893)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

ACTCAAGACTAACTGCGCCCCTTTTATTATGAAAAAATTTTTTAGTATTAGTGAGAATGTTTTTTTTCATGTTTTTATACCAGTGATTAGCCATCTGTGATTATATTATTTATGCTTTAAATAATTGTAAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCATTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAG  >  1/852092‑852565                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 gctcaagcgtAACTGCGCCCTTTTTATTACGAAAAAATTTTTTAATACTAGTGATGATATTTTTTTTCATGTTTTTATACCGGTGATTAACCATTTGCAATTATATTACTCGTGCTTTAAATAATCATGAAT‑‑‑‑TAATTATTTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGaaa                                                                                                                                                                                                                             >  1:267921/10‑251 (MQ=255)                                                                                                                                 tcatgaattaattattTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAAc                                                                                                                     >  1:768933/11‑231 (MQ=255)                                                                                                                                             tatttgttTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGaa                                                                                                                                                                                                >  1:78544/1‑144 (MQ=255)                                                                                                                                                 tgtttGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCttttgt                                                                                                                                  >  1:502462/3‑199 (MQ=255)                                                                                                                                                        cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                                                                          <  1:355380/235‑1 (MQ=255)                                                                                                                                                        cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                                                                          <  1:38067/235‑1 (MQ=255)                                                                                                                                                         ctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcaca                                                                         >  1:462618/16‑251 (MQ=255)                                                                                                                                                                         aTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGc                                                                                                                              >  1:129261/1‑182 (MQ=255)                                                                                                                                                                                   aTTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAAGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat                                               >  1:424603/1‑246 (MQ=255)                                                                                                                                                                                         ttcttGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcataaccg                                          >  1:164474/5‑240 (MQ=255)                                                                                                                                                                                                          aCTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCAttt                                    >  1:392231/1‑239 (MQ=255)                                                                                                                                                                                                                 taacaacaacctgcaaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAATTTCATACTTGTAGGTTAATGAATACCCAGCCAATTTTGCTAACCTACCATTGGGGGTTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAACCTTCCCATCTAATAAAAATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGAt                 <  1:649348/231‑1 (MQ=255)                                                                                                                                                                                                                           ctgctaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTat          >  1:483397/11‑247 (MQ=255)                                                                                                                                                                                                                             gctaacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAAc                                                          >  1:446746/9‑198 (MQ=255)                                                                                                                                                                                                                                aacgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATCCACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAACCTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCATTTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGagg  <  1:162447/246‑3 (MQ=255)                                                                                                                                                                                                                                  cgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTa                                                            <  1:839709/188‑1 (MQ=255)                                                                                                                                                                                                                                  cgttATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat                                               <  1:875529/201‑6 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 ACTCAAGACTAACTGCGCCCCTTTTATTATGAAAAAATTTTTTAGTATTAGTGAGAATGTTTTTTTTCATGTTTTTATACCAGTGATTAGCCATCTGTGATTATATTATTTATGCTTTAAATAATTGTAAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCATTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAG  >  1/852092‑852565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑