Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,279	C→T	intergenic (‑2622/‑69939)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,279	0	C	T	100.0%	22.0 / NA	10	intergenic (‑2622/‑69939)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

AAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTATTATGAAAAAATTTTTTAGTATTAGTGAGAATGTTTTTTTTCATGTTTTTATACCAGTGATTAGCCATCTGTGATTATATTATTTATGCTTTAAATAATTGTAAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCATTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGT  >  1/852071‑852520                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  aaaGAATAAGCATAAAAAGAAGCTCAAGCGTAACTGCGCCCTTTTTATTACGAAAAAATTTTTTAATACTAGTGATGATATTTTTTTTCATGTTTTTATACCGGTGATTAACCATTTGCAATTATATTACTCGTGCTTTAAATAATCATGAAT‑‑‑‑TAATTATTTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAActgctaacgttatccc                                                                                                                                                                                                     <  1:335287/251‑16 (MQ=255)                      gctcaagcgtAACTGCGCCCTTTTTATTACGAAAAAATTTTTTAATACTAGTGATGATATTTTTTTTCATGTTTTTATACCGGTGATTAACCATTTGCAATTATATTACTCGTGCTTTAAATAATCATGAAT‑‑‑‑TAATTATTTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGaaa                                                                                                                                                                                >  1:267921/10‑251 (MQ=255)                                                                                                                                                      tcatgaattaattattTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAAc                                                                        >  1:768933/11‑231 (MQ=255)                                                                                                                                                                  tatttgttTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGaa                                                                                                                                                   >  1:78544/1‑144 (MQ=255)                                                                                                                                                                      tgtttGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCttttgt                                                                                     >  1:502462/3‑199 (MQ=255)                                                                                                                                                                             cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                             <  1:355380/235‑1 (MQ=255)                                                                                                                                                                             cctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcac                             <  1:38067/235‑1 (MQ=255)                                                                                                                                                                              ctacctggatgctgaaaTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACcaca                            >  1:462618/16‑251 (MQ=255)                                                                                                                                                                                              aTTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAGGc                                                                                 >  1:129261/1‑182 (MQ=255)                                                                                                                                                                                                        aTTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAACTGCTAACGTTATCCCTAGGTAAAAATGGCAGCGAAACCAAAAATTCATACTTGTAGGTTAATGAATACACAGCCAATGTTGCTAACCTACCATTGGGGGGTATAATATTACCGTTTCTGTCTTTTGTAAGCTGCCTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAAtttcat  >  1:424603/1‑246 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  AAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTATTATGAAAAAATTTTTTAGTATTAGTGAGAATGTTTTTTTTCATGTTTTTATACCAGTGATTAGCCATCTGTGATTATATTATTTATGCTTTAAATAATTGTAAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCTTAGGTAAAAATGGCAGTGAAACCAGAAACTCATAATTGTAGGTCAATGAATATACAGCCAATGTTGCTAACCGACCATTGGGGGGTATAATCTTACCGTCTCTATCTTTAGTAGGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGT  >  1/852071‑852520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑