Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,075	T→A	intergenic (‑2418/‑70143)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,075	0	T	A	100.0%	11.3 / NA	6	intergenic (‑2418/‑70143)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (3/3);  total (3/3)

GCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTATTATGAAAAAATTTTTTAGTATTAGTGAGAATGTTTTTTTTCATGTTTTTATACCAGTGATTAGCCATCTGTGATTATATTATTTATGCTTTAAATAATTGTAAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCT  >  1/851837‑852325                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           gCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTATCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATaaaa                                                                                                                                                                                                                                                >  1:442647/1‑251 (MQ=255)   tCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCAGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAg                                                                                                                                                                                                                                              <  1:67448/251‑1 (MQ=255)              acCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAgcg                                                                                                                                                                                                                                   <  1:779644/251‑3 (MQ=255)                                gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAga                                                                                                                                                                                                                                             >  1:459000/1‑222 (MQ=255)                                gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAGCGTAACTGCGCCCTTTttat                                                                                                                                                                                                                 >  1:884263/1‑251 (MQ=255)                                                                                                                                                                                                                                           aaaGAATAAGCATAAAAAGAAGCTCAAGCGTAACTGCGCCCTTTTTATTACGAAAAAATTTTTTAATACTAGTGATGATATTTTTTTTCATGTTTTTATACCGGTGATTAACCATTTGCAATTATATTACTCGTGCTTTAAATAATCATGAAT‑‑‑‑TAATTATTTGTTTGACCTACCTGGATGCTGAAATTTAGATCTATTAAATTCTTGTACGGCTACAAACTTACGTGACAACAAActgctaacgttatccc  <  1:335287/251‑16 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           GCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTATTATGAAAAAATTTTTTAGTATTAGTGAGAATGTTTTTTTTCATGTTTTTATACCAGTGATTAGCCATCTGTGATTATATTATTTATGCTTTAAATAATTGTAAATTGCCTGATTATTTATTTGACCTGCCGGGATATTGGAATTTAGATCTATTAAATTCCTGTACGGCTACAAACTTACGTGACAATAAACCGCCAACACTATCCT  >  1/851837‑852325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑