Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,041	A→G	intergenic (‑2384/‑70177)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,041	0	A	G	100.0%	29.8 / NA	12	intergenic (‑2384/‑70177)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

GAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTAT  >  1/851798‑852118                                                                                                                                                                                                                                                    |                                                                              gattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGtt                                                                        >  1:651093/14‑251 (MQ=255)                             ctctTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAgaa                                            <  1:574359/251‑3 (MQ=255)                             ctctTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAgaa                                            <  1:779739/251‑3 (MQ=255)                                        gCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTATCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATaaaa                                 >  1:442647/1‑251 (MQ=255)                                          tCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCAGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAg                               <  1:67448/251‑1 (MQ=255)                                           cAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTc                                                                            <  1:664346/205‑1 (MQ=255)                                             aTAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATAc                                                    >  1:147517/1‑227 (MQ=255)                                                     acCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAgcg                    <  1:779644/251‑3 (MQ=255)                                                            ttATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTc                                                                            >  1:892138/1‑188 (MQ=255)                                                                       gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTaaaaagg                                                                 <  1:281579/188‑3 (MQ=255)                                                                       gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAga                              >  1:459000/1‑222 (MQ=255)                                                                       gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAGCGTAACTGCGCCCTTTttat  >  1:884263/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                              GAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTAT  >  1/851798‑852118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑