Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	851,981	C→T	intergenic (‑2324/‑70237)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	851,981	0	C	T	100.0%	76.8 / NA	30	intergenic (‑2324/‑70237)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/18);  total (12/18)

TAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTAT  >  1/851752‑852118                                                                                                                                                                                                                                      |                                                                                                                                          tAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGc                                                                                                                      <  1:794139/251‑1 (MQ=255)   agatagaTTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGAc                                                                                                                                     >  1:670121/5‑234 (MQ=255)             tcatCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttt                                                                                                          <  1:18778/249‑1 (MQ=255)             tcatCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttt                                                                                                          <  1:675512/249‑1 (MQ=255)                    aCTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttttccctgg                                                                                                   >  1:276791/1‑244 (MQ=255)                      ttACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                                                                                                 <  1:254629/251‑1 (MQ=255)                                       tgtgcctagattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAATGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTa                                                                                <  1:720347/230‑1 (MQ=255)                                               gattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGtt                                                                        >  1:651093/14‑251 (MQ=255)                                                 ttcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACt                                                                                 <  1:524724/229‑1 (MQ=255)                                                           tttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGaatacg                                                                                                                          <  1:126619/188‑6 (MQ=255)                                                                   ttttttttCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGCCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAAc                                                                                  <  1:431347/220‑1 (MQ=255)                                                                           ctctTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAgaa                                            <  1:779739/251‑3 (MQ=255)                                                                           ctctTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAgaa                                            <  1:574359/251‑3 (MQ=255)                                                                                      gCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTATCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATaaaa                                 >  1:442647/1‑251 (MQ=255)                                                                                        tCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCAGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAg                               <  1:67448/251‑1 (MQ=255)                                                                                         cAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTc                                                                            <  1:664346/205‑1 (MQ=255)                                                                                           aTAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATAc                                                    >  1:147517/1‑227 (MQ=255)                                                                                                gACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                                                                                                 <  1:682741/177‑1 (MQ=255)                                                                                                   acCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAgcg                    <  1:779644/251‑3 (MQ=255)                                                                                                       aCTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGt                                                                                                            <  1:568382/159‑1 (MQ=255)                                                                                                          ttATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTc                                                                            >  1:892138/1‑188 (MQ=255)                                                                                                           tATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGc                                                                                                                      <  1:33869/145‑1 (MQ=255)                                                                                                                     gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                                                                                                 <  1:577717/156‑1 (MQ=255)                                                                                                                     gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTAc                                                                                            >  1:360430/1‑161 (MQ=255)                                                                                                                     gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTa                                                                                >  1:589346/1‑173 (MQ=255)                                                                                                                     gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTaaaaagg                                                                 <  1:281579/188‑3 (MQ=255)                                                                                                                     gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAga                              >  1:459000/1‑222 (MQ=255)                                                                                                                     gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAGCGTAACTGCGCCCTTTttat  >  1:884263/1‑251 (MQ=255)                                                                                                                            tctcCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAAc                                                                                  >  1:158211/1‑164 (MQ=255)                                                                                                                                    caaaATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGaatacg                                                                                                                          >  1:790982/1‑111 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                          TAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTAT  >  1/851752‑852118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑