Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	851,973	A→G	intergenic (‑2316/‑70245)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	851,973	0	A	G	100.0%	84.1 / NA	32	intergenic (‑2316/‑70245)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/20);  total (12/20)

TTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTAT  >  1/851733‑852118                                                                                                                                                                                                                                                 |                                                                                                                                                  ttCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCgaagaatat                                                                                                                                         <  1:727878/251‑4 (MQ=255)                 aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCgaagaatat                                                                                                                                         <  1:225663/235‑4 (MQ=255)                    tAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGc                                                                                                                      <  1:794139/251‑1 (MQ=255)                      agatagaTTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGAc                                                                                                                                     >  1:670121/5‑234 (MQ=255)                                tcatCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttt                                                                                                          <  1:675512/249‑1 (MQ=255)                                tcatCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttt                                                                                                          <  1:18778/249‑1 (MQ=255)                                       aCTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttttccctgg                                                                                                   >  1:276791/1‑244 (MQ=255)                                         ttACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                                                                                                 <  1:254629/251‑1 (MQ=255)                                                          tgtgcctagattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAATGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTa                                                                                <  1:720347/230‑1 (MQ=255)                                                                  gattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGtt                                                                        >  1:651093/14‑251 (MQ=255)                                                                    ttcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACt                                                                                 <  1:524724/229‑1 (MQ=255)                                                                              tttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGaatacg                                                                                                                          <  1:126619/188‑6 (MQ=255)                                                                                      ttttttttCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGCCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAAc                                                                                  <  1:431347/220‑1 (MQ=255)                                                                                              ctctTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAgaa                                            <  1:779739/251‑3 (MQ=255)                                                                                              ctctTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAgaa                                            <  1:574359/251‑3 (MQ=255)                                                                                                         gCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTATCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATaaaa                                 >  1:442647/1‑251 (MQ=255)                                                                                                           tCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCAGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAg                               <  1:67448/251‑1 (MQ=255)                                                                                                            cAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTc                                                                            <  1:664346/205‑1 (MQ=255)                                                                                                              aTAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATAc                                                    >  1:147517/1‑227 (MQ=255)                                                                                                                   gACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                                                                                                 <  1:682741/177‑1 (MQ=255)                                                                                                                      acCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAgcg                    <  1:779644/251‑3 (MQ=255)                                                                                                                          aCTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGt                                                                                                            <  1:568382/159‑1 (MQ=255)                                                                                                                             ttATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTc                                                                            >  1:892138/1‑188 (MQ=255)                                                                                                                              tATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGc                                                                                                                      <  1:33869/145‑1 (MQ=255)                                                                                                                                        gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                                                                                                 <  1:577717/156‑1 (MQ=255)                                                                                                                                        gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTAc                                                                                            >  1:360430/1‑161 (MQ=255)                                                                                                                                        gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTa                                                                                >  1:589346/1‑173 (MQ=255)                                                                                                                                        gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTaaaaagg                                                                 <  1:281579/188‑3 (MQ=255)                                                                                                                                        gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAga                              >  1:459000/1‑222 (MQ=255)                                                                                                                                        gTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGTTAAAAAGGCGAAAATAAATACTAAAAGAATAAGCATAAAAAGAAGCTCAAGCGTAACTGCGCCCTTTttat  >  1:884263/1‑251 (MQ=255)                                                                                                                                               tctcCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAAc                                                                                  >  1:158211/1‑164 (MQ=255)                                                                                                                                                       caaaATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGaatacg                                                                                                                          >  1:790982/1‑111 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                  TTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTTAAAAAAGCGAAAATAAATACTAAAAGTATAAGCATAAAAAGGAACTCAAGACTAACTGCGCCCCTTTTAT  >  1/851733‑852118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑