Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	851,818	C→T	intergenic (‑2161/‑70400)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	851,818	0	C	T	100.0%	210.2 / NA	73	intergenic (‑2161/‑70400)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/48);  total (25/48)

TGCCAGTGAAGAGGAACTTAGCATACCAAATGACTGGTTTTCTTTGCGTGTTATTAAATTTTTAAAAAAGCTACCTGCTTCAACACAAAGTGTTACCTGATACAGTGGAATTTTTCTTTGGTTGTCAGGGTTTGCTGCTTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTT  >  1/851595‑852048                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       tgctaGTGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAg                                                                                                                                                                                                             <  1:89932/247‑1 (MQ=255)       tGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGacac                                                                                                                                                                                                       <  1:53332/251‑1 (MQ=255)       tGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGaca                                                                                                                                                                                                        <  1:489688/250‑1 (MQ=255)        gAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGacac                                                                                                                                                                                                       >  1:15138/1‑250 (MQ=255)              gAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACttt                                                                                                                                                                                                >  1:462529/1‑251 (MQ=255)                aCTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                                                                                                                                          <  1:424911/239‑1 (MQ=255)                  ttaacATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTAttt                                                                                                                                                                                            <  1:85235/247‑1 (MQ=255)                     acATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTgga                                                                                                                                                                                         >  1:81485/2‑250 (MQ=255)                      cATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTggac                                                                                                                                                                                        <  1:135509/251‑3 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGGCAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              <  1:852696/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              <  1:888826/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              <  1:869934/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              <  1:840927/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              <  1:30384/250‑1 (MQ=255)                                  cTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtctc                                                                                                                                                                            <  1:55804/251‑1 (MQ=255)                                    ggTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCca                                                                                                                                                                          >  1:744314/1‑251 (MQ=255)                                       tttCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCcaaac                                                                                                                                                                       <  1:273175/251‑1 (MQ=255)                                          cTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTAttt                                                                                                                                                                                            <  1:138188/227‑1 (MQ=255)                                          cTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCcaaacaaa                                                                                                                                                                    >  1:205728/1‑251 (MQ=255)                                                gtgtTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGt                                                                                                                                                              >  1:644617/1‑251 (MQ=255)                                                 tgtTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGtt                                                                                                                                                             <  1:877908/251‑1 (MQ=255)                                                                             gCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagt                                                                                                                                  <  1:452542/250‑3 (MQ=255)                                                                                          gggtcaCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGt                                                                                                                    >  1:316086/6‑251 (MQ=255)                                                                                               aCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAg                                                                                                                                                               >  1:248754/1‑203 (MQ=255)                                                                                               aCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAg                                                                                                                               >  1:158633/1‑235 (MQ=255)                                                                                               aCCTGATACAGCGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGttttt                                                                                                                >  1:621494/1‑246 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                                                                                                                                          <  1:465057/159‑1 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcg                                                                                                              >  1:421216/1‑245 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcg                                                                                                              >  1:802435/1‑245 (MQ=255)                                                                                                    aTACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              >  1:266892/1‑183 (MQ=255)                                                                                                            ggaTTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATc                                                                                                   <  1:154306/248‑1 (MQ=255)                                                                                                              aTTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCAt                                                                                                 <  1:707405/250‑1 (MQ=255)                                                                                                                 tttCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGta                                                                                             <  1:786500/251‑1 (MQ=255)                                                                                                                  ttCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtat                                                                                            <  1:694374/251‑1 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         <  1:560506/251‑3 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         <  1:760385/251‑3 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         <  1:97614/251‑3 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         <  1:228923/251‑3 (MQ=255)                                                                                                                       tGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg                                                                                       <  1:297535/251‑5 (MQ=255)                                                                                                                       tGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg                                                                                       <  1:349469/251‑5 (MQ=255)                                                                                                                         gTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              >  1:684249/1‑162 (MQ=255)                                                                                                                            gTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                                                                                              <  1:732628/159‑1 (MQ=255)                                                                                                                                gggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                                                                                                                                          <  1:609688/127‑1 (MQ=255)                                                                                                                                gggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAATTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtt                                                                                                                   >  1:433342/1‑213 (MQ=255)                                                                                                                                 ggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg                                                                                       <  1:473794/241‑5 (MQ=255)                                                                                                                                 ggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACgct                                                                               <  1:385261/249‑3 (MQ=255)                                                                                                                                      gctgatTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagtc                                                                                                                                 >  1:842431/1‑191 (MQ=255)                                                                                                                                           ttCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCgaagaatat                                                                   <  1:727878/251‑4 (MQ=255)                                                                                                                                               gAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagtc                                                                                                                                 >  1:316959/1‑182 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagt                                                                                                                                  <  1:8040/172‑3 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcggtcccatta                                                                                                     >  1:685021/1‑186 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcggtcccatt                                                                                                      <  1:116916/200‑15 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg                                                                                       <  1:345273/215‑5 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         <  1:801464/213‑3 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         >  1:2374/1‑211 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCgaagaatat                                                                   <  1:225663/235‑4 (MQ=255)                                                                                                                                                           aaaTAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTCTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac                                                                                         <  1:789046/213‑3 (MQ=255)                                                                                                                                                              tAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGc                                                <  1:794139/251‑1 (MQ=255)                                                                                                                                                                agatagaTTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGAc                                                               >  1:670121/5‑234 (MQ=255)                                                                                                                                                                 gatagaTTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGt                                                                                                                                       >  1:193652/4‑161 (MQ=255)                                                                                                                                                                    agaTTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagtc                                                                                                                                 <  1:809505/164‑4 (MQ=255)                                                                                                                                                                     gaTTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAGCTGATTACg                                                                                 <  1:539316/211‑1 (MQ=255)                                                                                                                                                                          tcatCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttt                                    <  1:675512/249‑1 (MQ=255)                                                                                                                                                                          tcatCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttt                                    <  1:18778/249‑1 (MQ=255)                                                                                                                                                                                 aCTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTTCAAACAAAATAAGtt                                                                                                                                                             >  1:473485/1‑123 (MQ=255)                                                                                                                                                                                 aCTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcggtcc                                                                                                          >  1:875960/1‑164 (MQ=255)                                                                                                                                                                                 aCTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGttttccctgg                             >  1:276791/1‑244 (MQ=255)                                                                                                                                                                                   ttACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGt                           <  1:254629/251‑1 (MQ=255)                                                                                                                                                                                                    tgtgcctagattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAATGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTa          <  1:720347/230‑1 (MQ=255)                                                                                                                                                                                                            gattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCa                                                                                                  <  1:492385/142‑1 (MQ=255)                                                                                                                                                                                                            gattcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACTAGGTCTGtt  >  1:651093/14‑251 (MQ=255)                                                                                                                                                                                                              ttcgttaacatttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGAATACGAGGCATTGTCCAGTTTTCCCTGGGTAGTACGAACAATAACt           <  1:524724/229‑1 (MQ=255)                                                                                                                                                                                                                        tttCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACGCTCGCGAAGAATATTGACCAGTGaatacg                                                    <  1:126619/188‑6 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       TGCCAGTGAAGAGGAACTTAGCATACCAAATGACTGGTTTTCTTTGCGTGTTATTAAATTTTTAAAAAAGCTACCTGCTTCAACACAAAGTGTTACCTGATACAGTGGAATTTTTCTTTGGTTGTCAGGGTTTGCTGCTTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTTCACGAAGAACATTGACCAGTGAGTATGAGGCATTGTCCAGTTTCCCTTGGGTCGTACGAACAATAACTAAGTCTGTT  >  1/851595‑852048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑