Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	851,732	C→A	intergenic (‑2075/‑70486)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	851,732	0	C	A	100.0%	124.0 / NA	46	intergenic (‑2075/‑70486)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/31);  total (15/31)

TGCCAGTGAAGAGGAACTTAGCATACCAAATGACTGGTTTTCTTTGCGTGTTATTAAATTTTTAAAAAAGCTACCTGCTTCAACACAAAGTGTTACCTGATACAGTGGAATTTTTCTTTGGTTGTCAGGGTTTGCTGCTTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTT  >  1/851595‑851971                                                                                                                                          |                                                                                                                                                                                                                                                tgctaGTGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAg                                                                                                                                <  1:89932/247‑1 (MQ=255)       tGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGacac                                                                                                                          <  1:53332/251‑1 (MQ=255)       tGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGaca                                                                                                                           <  1:489688/250‑1 (MQ=255)        gAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGacac                                                                                                                          >  1:15138/1‑250 (MQ=255)              gAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACttt                                                                                                                   >  1:462529/1‑251 (MQ=255)                aCTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                                                             <  1:424911/239‑1 (MQ=255)                  ttaacATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTAttt                                                                                                               <  1:85235/247‑1 (MQ=255)                     acATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTgga                                                                                                            >  1:81485/2‑250 (MQ=255)                      cATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTggac                                                                                                           <  1:135509/251‑3 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGGCAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 <  1:852696/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 <  1:840927/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 <  1:30384/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 <  1:869934/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 <  1:888826/250‑1 (MQ=255)                                  cTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtctc                                                                                               <  1:55804/251‑1 (MQ=255)                                    ggTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCca                                                                                             >  1:744314/1‑251 (MQ=255)                                       tttCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCcaaac                                                                                          <  1:273175/251‑1 (MQ=255)                                          cTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTAttt                                                                                                               <  1:138188/227‑1 (MQ=255)                                          cTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCcaaacaaa                                                                                       >  1:205728/1‑251 (MQ=255)                                                gtgtTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGt                                                                                 >  1:644617/1‑251 (MQ=255)                                                 tgtTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGtt                                                                                <  1:877908/251‑1 (MQ=255)                                                                             gCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagt                                                     <  1:452542/250‑3 (MQ=255)                                                                                          gggtcaCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGt                                       >  1:316086/6‑251 (MQ=255)                                                                                               aCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAg                                                                                  >  1:248754/1‑203 (MQ=255)                                                                                               aCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAg                                                  >  1:158633/1‑235 (MQ=255)                                                                                               aCCTGATACAGCGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGttttt                                   >  1:621494/1‑246 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                                                             <  1:465057/159‑1 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcg                                 >  1:421216/1‑245 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcg                                 >  1:802435/1‑245 (MQ=255)                                                                                                    aTACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 >  1:266892/1‑183 (MQ=255)                                                                                                            ggaTTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATc                      <  1:154306/248‑1 (MQ=255)                                                                                                              aTTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCAt                    <  1:707405/250‑1 (MQ=255)                                                                                                                 tttCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGta                <  1:786500/251‑1 (MQ=255)                                                                                                                  ttCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtat               <  1:694374/251‑1 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac            <  1:228923/251‑3 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac            <  1:760385/251‑3 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac            <  1:560506/251‑3 (MQ=255)                                                                                                                     tttGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataac            <  1:97614/251‑3 (MQ=255)                                                                                                                       tGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg          <  1:297535/251‑5 (MQ=255)                                                                                                                       tGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg          <  1:349469/251‑5 (MQ=255)                                                                                                                         gTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 >  1:684249/1‑162 (MQ=255)                                                                                                                            gTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                                                 <  1:732628/159‑1 (MQ=255)                                                                                                                                gggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                                                             <  1:609688/127‑1 (MQ=255)                                                                                                                                gggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAATTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtt                                      >  1:433342/1‑213 (MQ=255)                                                                                                                                 ggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGtataactg          <  1:473794/241‑5 (MQ=255)                                                                                                                                 ggTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGTTTTTCGGTCCCATTATCATTGTATAACTGATTACgct  <  1:385261/249‑3 (MQ=255)                                                                                                                                      gctgatTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagtc                                                    >  1:842431/1‑191 (MQ=255)                                                                                                                                          |                                                                                                                                                                                                                                                TGCCAGTGAAGAGGAACTTAGCATACCAAATGACTGGTTTTCTTTGCGTGTTATTAAATTTTTAAAAAAGCTACCTGCTTCAACACAAAGTGTTACCTGATACAGTGGAATTTTTCTTTGGTTGTCAGGGTTTGCTGCTTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTGCTCCCATTATCATTGTATAGTTGATTACGTT  >  1/851595‑851971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑