Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	851,703	A→G	intergenic (‑2046/‑70515)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	851,703	0	A	G	100.0%	80.9 / NA	30	intergenic (‑2046/‑70515)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

TGCCAGTGAAGAGGAACTTAGCATACCAAATGACTGGTTTTCTTTGCGTGTTATTAAATTTTTAAAAAAGCTACCTGCTTCAACACAAAGTGTTACCTGATACAGTGGAATTTTTCTTTGGTTGTCAGGGTTTGCTGCTTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTG  >  1/851595‑851940                                                                                                             |                                                                                                                                                                                                                                              tgctaGTGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAg                                                                                                 <  1:89932/247‑1 (MQ=255)       tGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGacac                                                                                           <  1:53332/251‑1 (MQ=255)       tGAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGaca                                                                                            <  1:489688/250‑1 (MQ=255)        gAAGAGGAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGacac                                                                                           >  1:15138/1‑250 (MQ=255)              gAACTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACttt                                                                                    >  1:462529/1‑251 (MQ=255)                aCTTAACATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                              <  1:424911/239‑1 (MQ=255)                  ttaacATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTAttt                                                                                <  1:85235/247‑1 (MQ=255)                     acATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTgga                                                                             >  1:81485/2‑250 (MQ=255)                      cATACCAAATGACTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTggac                                                                            <  1:135509/251‑3 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGGCAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                  <  1:852696/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                  <  1:888826/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                  <  1:869934/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                  <  1:30384/250‑1 (MQ=255)                                 aCTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                  <  1:840927/250‑1 (MQ=255)                                  cTGGTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtctc                                                                <  1:55804/251‑1 (MQ=255)                                    ggTTTTCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCca                                                              >  1:744314/1‑251 (MQ=255)                                       tttCTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCcaaac                                                           <  1:273175/251‑1 (MQ=255)                                          cTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTAttt                                                                                <  1:138188/227‑1 (MQ=255)                                          cTTTACGTGTTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCcaaacaaa                                                        >  1:205728/1‑251 (MQ=255)                                                gtgtTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGt                                                  >  1:644617/1‑251 (MQ=255)                                                 tgtTATTAAATTTTTGAAAAAGCTACCGGCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGtt                                                 <  1:877908/251‑1 (MQ=255)                                                                             gCTTCAACGCAAAGGGTCACCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTaagt                      <  1:452542/250‑3 (MQ=255)                                                                                          gggtcaCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGt        >  1:316086/6‑251 (MQ=255)                                                                                               aCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAg                                                   >  1:248754/1‑203 (MQ=255)                                                                                               aCCTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAg                   >  1:158633/1‑235 (MQ=255)                                                                                               aCCTGATACAGCGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGttttt    >  1:621494/1‑246 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGa                                                                                              <  1:465057/159‑1 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcg  >  1:802435/1‑245 (MQ=255)                                                                                                ccTGATACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAGTCAGTACTTTTTAGtttttcg  >  1:421216/1‑245 (MQ=255)                                                                                                    aTACAGTGGGATTTTTCTTTGGTTGTCAGGGTTAGCTGATTCTGAGCGTGGCGACAAATAAGATAGATTATCATCCAACTTACGATAAGGCCGACATGTGCCTAGATTCGTTAACATTTCAGGATTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGACTGTCTTTAtc                                                                  >  1:266892/1‑183 (MQ=255)                                                                                                             |                                                                                                                                                                                                                                              TGCCAGTGAAGAGGAACTTAGCATACCAAATGACTGGTTTTCTTTGCGTGTTATTAAATTTTTAAAAAAGCTACCTGCTTCAACACAAAGTGTTACCTGATACAGTGGAATTTTTCTTTGGTTGTCAGGGTTTGCTGCTTCTGAGCGTGGTGACAAATAAGAGAGATTATTATCCAACTTACGATAAGGCTGGCAGGTACTTAGAGTCGTTAATGATTCAGGACTTTTTTTCTCTTCCCAGTGCTCAATAGTGACACCGACTTTATTTGGGCTGTCTTTATCTCCAAACAAAATAAGTTTTGCCATTTTTTCATATTCAGTTAAATCAGTACTTTTTAGTGGTTTG  >  1/851595‑851940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑