Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	846,908	T→C	intergenic (‑26231/+2673)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	846,908	0	T	C	100.0%	216.0 / NA	78	intergenic (‑26231/+2673)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/38);  total (40/38)

GCACGGTTGTTGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGTCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGCCTCGCC  >  1/846680‑847140                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         gCACGGTTGTTGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGATTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATAttctt                                                                                                                                                                                                                     >  1:448984/1‑250 (MQ=255)      gttgttGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAGATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATAttcttc                                                                                                                                                                                                                    >  1:797179/1‑246 (MQ=255)      gttgttGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTccc                                                                                                                                                                                                                <  1:73348/250‑1 (MQ=255)      gttgttGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTccc                                                                                                                                                                                                                <  1:546757/250‑1 (MQ=255)      gttgttGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTcc                                                                                                                                                                                                                 >  1:234432/1‑249 (MQ=255)      gttgttGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTcc                                                                                                                                                                                                                 >  1:605677/1‑249 (MQ=255)             gTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCAtt                                                                                                                                                                                                        >  1:519948/1‑251 (MQ=255)             gTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCAtt                                                                                                                                                                                                        >  1:19830/1‑251 (MQ=255)             gTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCAt                                                                                                                                                                                                         <  1:829740/250‑1 (MQ=255)                 gTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGtt                                                                                                                                                                                                    >  1:138486/1‑251 (MQ=255)                         ccGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGtatt                                                                                                                                                                                            >  1:269313/1‑251 (MQ=255)                             ttGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATgg                                                                                                                                                                                        <  1:562121/251‑1 (MQ=255)                             ttGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATgg                                                                                                                                                                                        <  1:714005/251‑1 (MQ=255)                               ggCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTcc                                                                                                                                                                                                                 >  1:24652/1‑224 (MQ=255)                                gCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATgg                                                                                                                                                                                        <  1:276111/248‑1 (MQ=255)                                  tAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGAcc                                                                                                                                                                                   <  1:36005/251‑1 (MQ=255)                                       aCTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGc                                                                                                                                                                              <  1:339600/251‑1 (MQ=255)                                       aCTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTAAGGTGACCTATg                                                                                                                                                                               >  1:765098/1‑250 (MQ=255)                                        cTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGAc                                                                                                                                                                                                                               >  1:829132/1‑201 (MQ=255)                                           tatttattTTATCCCTCGGTGTTCAAAAAGGTGTTGCGCGCACTTCTGTTATTTTTTTTGCAATTTTAGTCATCATGTTTTTCTCTTTAATGGTTTATTCCTTATTTTTACCGGGGGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTcc                                                                                                                                                                          <  1:847933/251‑1 (MQ=255)                                           tATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAG‑AAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCt                                                                                                                                                                         <  1:495096/251‑1 (MQ=255)                                                 ttttATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTg                                                                                                                                                                    >  1:502285/1‑251 (MQ=255)                                                       cccTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTg                                                                                                                                                                                                      <  1:193073/211‑1 (MQ=255)                                                             gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATAttcttc                                                                                                                                                                                                                    <  1:96024/191‑1 (MQ=255)                                                             gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCg                                                                                                                                                        >  1:865820/1‑251 (MQ=255)                                                             gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCg                                                                                                                                                        >  1:155287/1‑251 (MQ=255)                                                             gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCg                                                                                                                                                        >  1:734158/1‑251 (MQ=255)                                                                    aaaaGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTaa                                                                                                                                                 >  1:391427/1‑251 (MQ=255)                                                                      aaGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAc                                                                                                                                                >  1:85995/1‑250 (MQ=255)                                                                      aaGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTCACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAcc                                                                                                                                               <  1:447954/251‑1 (MQ=255)                                                                                   cACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGAc                                                                                                                                                                                    >  1:888698/1‑201 (MQ=255)                                                                                     cTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGAcc                                                                                                                                                                                   >  1:644294/1‑200 (MQ=255)                                                                                     cTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTtggtg                                                                                                                                >  1:342428/1‑251 (MQ=255)                                                                                     cTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTtggtg                                                                                                                                >  1:682560/1‑251 (MQ=255)                                                                                           tgATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGc                                                                                                                          <  1:897206/251‑1 (MQ=255)                                                                                           tgATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGc                                                                                                                          <  1:805785/251‑1 (MQ=255)                                                                                                  tATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCa                                                                                                                   <  1:561454/251‑1 (MQ=255)                                                                                                         aTTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAAcagcag                                                                                                            >  1:289935/1‑251 (MQ=255)                                                                                                         aTTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAAcagcag                                                                                                            >  1:431586/1‑251 (MQ=255)                                                                                                                  atcatGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCg                                                                                                                                              <  1:6641/208‑1 (MQ=255)                                                                                                                   tcatGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTTGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAgtg                                                                                                  <  1:68666/251‑1 (MQ=255)                                                                                                                   tcatGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAgtg                                                                                                  >  1:747729/1‑251 (MQ=255)                                                                                                                       gTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTgg                                                                                              >  1:855619/1‑251 (MQ=255)                                                                                                                               cTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTg                                                                                                                                                                                                      <  1:293693/139‑1 (MQ=255)                                                                                                                               cTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGta                                                                                                                                                                                              >  1:403680/1‑147 (MQ=255)                                                                                                                               cTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCtt                                                                                                                        <  1:415396/217‑1 (MQ=255)                                                                                                                                   aaTGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCg                                                                                  <  1:867532/251‑1 (MQ=255)                                                                                                                                      ggTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTg                                                                                                                                                                                      <  1:323792/148‑1 (MQ=255)                                                                                                                                      ggTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTAt                                                                               >  1:268306/1‑251 (MQ=255)                                                                                                                                       gTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATggctg                                                                                                                                                                                     <  1:137366/148‑4 (MQ=255)                                                                                                                                       gTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTAtt                                                                              >  1:196502/1‑251 (MQ=255)                                                                                                                                        tttATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATtc                                                                             <  1:599736/251‑1 (MQ=255)                                                                                                                                              ccTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGc                                                                        >  1:156106/1‑250 (MQ=255)                                                                                                                                                 tATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTa                                                                    >  1:357466/1‑251 (MQ=255)                                                                                                                                                    ttttACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGt                                                                 <  1:329743/251‑1 (MQ=255)                                                                                                                                                    ttttACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGt                                                                 <  1:462925/251‑1 (MQ=255)                                                                                                                                                            ggCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTg                                                          <  1:804043/250‑1 (MQ=255)                                                                                                                                                              cgcAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCg                                                       <  1:841252/251‑1 (MQ=255)                                                                                                                                                                  gAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTg                                                    >  1:189282/1‑250 (MQ=255)                                                                                                                                                                        gggTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGc                                                                                                                                                                              >  1:52832/1‑122 (MQ=255)                                                                                                                                                                        gggTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTa                                                                                                                                                                        <  1:268601/128‑1 (MQ=255)                                                                                                                                                                        gggTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACgg                                             >  1:191257/1‑251 (MQ=255)                                                                                                                                                                                 gCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAcc                                                                                                                                               <  1:768161/144‑1 (MQ=255)                                                                                                                                                                                 gCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGGATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGAtgt                                    >  1:550393/1‑251 (MQ=255)                                                                                                                                                                                    cTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAg                                 <  1:753167/251‑1 (MQ=255)                                                                                                                                                                                      gTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCg                               >  1:487963/1‑251 (MQ=255)                                                                                                                                                                                       ttCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCg                               <  1:141480/250‑1 (MQ=255)                                                                                                                                                                                         ctcGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAg                            <  1:160255/251‑1 (MQ=255)                                                                                                                                                                                                gATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGaca                                          >  1:191698/1‑230 (MQ=255)                                                                                                                                                                                                gATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCaa                      <  1:843432/250‑1 (MQ=255)                                                                                                                                                                                                          aaTTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCt                                                                                                                                                                         <  1:486052/93‑1 (MQ=255)                                                                                                                                                                                                          aaTTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGtata           >  1:457180/1‑251 (MQ=255)                                                                                                                                                                                                            ttAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAgg         <  1:565518/251‑1 (MQ=255)                                                                                                                                                                                                             tAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCg                               <  1:159245/228‑1 (MQ=255)                                                                                                                                                                                                             tAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAggt        >  1:357597/1‑250 (MQ=255)                                                                                                                                                                                                              aaaaGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCACCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAggtc       <  1:876590/251‑3 (MQ=255)                                                                                                                                                                                                                   aCCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGcc                                                  >  1:685017/1‑203 (MQ=255)                                                                                                                                                                                                                          cGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGcc  >  1:827705/1‑244 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         GCACGGTTGTTGGTCCGTTACTTGCCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGTCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGCCTCGCC  >  1/846680‑847140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑