Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	845,636	C→T	intergenic (‑24959/+3945)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	845,636	0	C	T	100.0%	181.4 / NA	68	intergenic (‑24959/+3945)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/32);  total (36/32)

ACCATGGCGTCGAAATAAAACCCCGCGCCGGAAGCAATGCCGATACCTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGCTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCATAAGATTTAAGT  >  1/845393‑845876                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 aCCATGGCGTCGAAATAAAACCCCGCGCCGGAAGCAATGCCGATACCTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTg                                                                                                                                                                                                                                            <  1:332368/250‑1 (MQ=255)                             cGGAAGCAATGCCGATACCTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAAcc                                                                                                                                                                                                               <  1:262213/251‑1 (MQ=255)                                   cAATGCCGATACCTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGatat                                                                                                                                                                                                         <  1:30755/251‑1 (MQ=255)                                               cTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTc                                                                                                                                                                                             <  1:139519/251‑1 (MQ=255)                                               cTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTc                                                                                                                                                                                             <  1:98244/251‑1 (MQ=255)                                               cTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTc                                                                                                                                                                                             <  1:553185/251‑1 (MQ=255)                                                     ccgccCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTgcgc                                                                                                                                                                                        <  1:175202/250‑1 (MQ=255)                                                              atcatcGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGAt                                                                                                                                                                              <  1:298952/251‑1 (MQ=255)                                                                  tcGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGAt                                                                                                                                                                                                                                          <  1:663037/187‑1 (MQ=255)                                                                  tcGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTgcg                                                                                                                                                                          >  1:306998/1‑251 (MQ=255)                                                                     cAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGcc                                                                                                                                                                        >  1:371394/1‑250 (MQ=255)                                                                             aTTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCaa                                                                                                                                                               <  1:3895/251‑1 (MQ=255)                                                                              ttttGGCGAAAATAAAAAAGAGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAAt                                                                                                                                                              >  1:315239/1‑251 (MQ=255)                                                                                            aaaaaGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGcc                                                                                                                                                <  1:56770/251‑1 (MQ=255)                                                                                                 gTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCaa                                                                                                                                                               >  1:739223/1‑231 (MQ=255)                                                                                                             cgcCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATg                                                                                                                                <  1:567894/250‑1 (MQ=255)                                                                                                                     cTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGCGGGAATATGTTTGCGtt                                                                                                                        >  1:217890/1‑250 (MQ=255)                                                                                                                           tAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAAt                                                                                                                 <  1:346525/251‑1 (MQ=255)                                                                                                                              aaTTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTg                                                                                                                                        <  1:366932/225‑1 (MQ=255)                                                                                                                                        attattCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGt                                                                                                    >  1:67339/1‑251 (MQ=255)                                                                                                                                          tattCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATg                                                                                                                                <  1:14268/221‑1 (MQ=255)                                                                                                                                                tGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTg                                                                                                                                                                                                                                            >  1:858036/1‑107 (MQ=255)                                                                                                                                                             aTTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATaaaa                                                                                          >  1:248973/1‑240 (MQ=255)                                                                                                                                                                tAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGaa                                                                            >  1:813495/1‑251 (MQ=255)                                                                                                                                                                 aaGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTtcatc                                                                                                                                                                 >  1:195232/1‑165 (MQ=255)                                                                                                                                                                   gTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAAt                                                                          >  1:856457/1‑250 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATg                                                                                                                                                                                                        <  1:675418/122‑1 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTtcatc                                                                                                                                                                 >  1:122566/1‑161 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGcc                                                                                                                                                <  1:579127/178‑1 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGc                                                                                                      <  1:362916/220‑1 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATc                                                                         <  1:629079/249‑1 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCaa                                                                       >  1:450719/1‑251 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCaa                                                                       >  1:751469/1‑251 (MQ=255)                                                                                                                                                                     aaTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCaa                                                                       >  1:529775/1‑251 (MQ=255)                                                                                                                                                                      aTTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCaa                                                                       >  1:855710/1‑250 (MQ=255)                                                                                                                                                                       agacagcAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAAc                                                                      >  1:77631/7‑250 (MQ=255)                                                                                                                                                                         gATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTc                                                                                                                                                                                             >  1:715315/1‑129 (MQ=255)                                                                                                                                                                          aTACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTg                                                                                                                                                                            >  1:92729/1‑145 (MQ=255)                                                                                                                                                                          aTACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGaa                                                                                                                                    <  1:109232/185‑1 (MQ=255)                                                                                                                                                                          aTACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATg                                                                                                                                >  1:287814/1‑189 (MQ=255)                                                                                                                                                                          aTACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATg                                                                                                       >  1:560938/1‑214 (MQ=255)                                                                                                                                                                          aTACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCa                                                                  >  1:634216/1‑251 (MQ=255)                                                                                                                                                                           tACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCAc                                                                 <  1:560481/251‑1 (MQ=255)                                                                                                                                                                            aCAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGtt                                                                                                                              >  1:415755/1‑189 (MQ=255)                                                                                                                                                                               gTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGtt                                                                                                                              <  1:83051/186‑1 (MQ=255)                                                                                                                                                                               gTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACtttt                                                             >  1:852462/1‑251 (MQ=255)                                                                                                                                                                               gTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACtttt                                                             >  1:519358/1‑251 (MQ=255)                                                                                                                                                                               gTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGCGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACtttt                                                             >  1:239311/1‑251 (MQ=255)                                                                                                                                                                               gTTCTCTTAGTTCGTTCTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGtt                                                                                                                              <  1:452151/186‑1 (MQ=255)                                                                                                                                                                                  ctctTAGTTTGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAata                                                                                                                                  <  1:448134/179‑1 (MQ=255)                                                                                                                                                                                  ctctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGAt                                                                                                                                                                              >  1:298182/1‑135 (MQ=255)                                                                                                                                                                                  ctctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGtt                                                                                                                              <  1:330473/183‑1 (MQ=255)                                                                                                                                                                                  ctctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCa                                                          >  1:11874/1‑251 (MQ=255)                                                                                                                                                                                  ctctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCa                                                          >  1:727291/1‑251 (MQ=255)                                                                                                                                                                                  ctctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCa                                                          >  1:685683/1‑251 (MQ=255)                                                                                                                                                                                  ctctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCa                                                          >  1:598401/1‑251 (MQ=255)                                                                                                                                                                                   tctTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAata                                                                                                                                  <  1:711055/178‑1 (MQ=255)                                                                                                                                                                                     ttAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGAt                                                       <  1:735449/251‑1 (MQ=255)                                                                                                                                                                                       aGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGAttt                                                     >  1:534613/1‑251 (MQ=255)                                                                                                                                                                                                 gAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGaaa                                                                           <  1:768806/219‑1 (MQ=255)                                                                                                                                                                                                 gAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATc                                                                         >  1:395843/1‑221 (MQ=255)                                                                                                                                                                                                                  gTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACt                                                                                                                                                       >  1:449101/1‑126 (MQ=255)                                                                                                                                                                                                                   ttttCGGCATCAAATTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAagag                          <  1:206375/250‑1 (MQ=255)                                                                                                                                                                                                                               aaTTTTGCAGGTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCAt             <  1:757282/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCATAAGATTtaa    >  1:325683/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCATAAGATTtaag   >  1:822176/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCATAAGATTtaagt  <  1:623292/251‑1 (MQ=255)                                                                                                                                                                                                                                          ttGAATCCCGTTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCATAAGATTtaag   <  1:854031/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ACCATGGCGTCGAAATAAAACCCCGCGCCGGAAGCAATGCCGATACCTGCCGCCGCCCAGAATCATCGCAAACGCCATTTTGGCGAAAATAAAAAAGTGGCTGTCGCGCGCCGACGCTTCAATAAAATTTAATAAATTATTCATGTTAAATCGTTAATTTAAGTAATTGATACAGTTCTCTTAGTTCGTTTTGAGTTAAAGTGCGGTAGGTTTTCGGCATCAAATTTTGCAGGTTGAATCCCGCTAATTGATAACGAATCAGACGTAACGTCGGAAACCCGATATGCGCCGTCATTCTGCGCACCTGCCGATTGCGCCCTTCATCAATCTTCACTTCCAGCCACGCGGTGGGAATATGTTTGCGTTCGCGAATAGGCGAAATGCGTTGCCATAAAAAATCCGGTTCGGAAATCAACCGCACTTTTGCAGATTTTCGCCTCGTTTTTTTGTCGCTGAAGAGATGTCGGTTGCATAAGATTTAAGT  >  1/845393‑845876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑