Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	833,532	A→G	intergenic (‑12855/+16049)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	833,532	0	A	G	100.0%	225.1 / NA	78	intergenic (‑12855/+16049)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/48);  total (30/48)

CGGACGCGCCAATGCCATCAAATTGCTGGCTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTACAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGCGGCAAAGAATATGTT  >  1/833286‑833751                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            cGGACGCGCCAATGCCATCAAATTGCTGGTTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAAc                                                                                                                                                                                                                         <  1:42414/251‑1 (MQ=255) cGGACGCGCCAATGCCATCAAATTGCTGGTTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAAc                                                                                                                                                                                                                         <  1:172452/251‑1 (MQ=255)       cgcCAATGCCATCAAATTGCTGGTTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTg                                                                                                                                                                                                                   >  1:100334/1‑251 (MQ=255)          cAATGCCATCAAATTGCTGGTTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATa                                                                                                                                                                                                                <  1:886670/251‑1 (MQ=255)                 aTCAAATTGCTGGTTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGa                                                                                                                                                                                                          >  1:529847/1‑250 (MQ=255)                     aaTTGCTGGTTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAg                                                                                                                                                                                                     >  1:543305/1‑251 (MQ=255)                              ttCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTcaca                                                                                                                                                                                            <  1:595601/250‑1 (MQ=255)                               tCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCacac                                                                                                                                                                                           <  1:297129/251‑1 (MQ=255)                                       tGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTTATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   <  1:49118/251‑1 (MQ=255)                                       tGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   <  1:27416/251‑1 (MQ=255)                                       tGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   <  1:304994/251‑1 (MQ=255)                                       tGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   <  1:289538/251‑1 (MQ=255)                                       tGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   <  1:17232/251‑1 (MQ=255)                                         gCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACca                                                                                                                                                                                  >  1:389140/1‑250 (MQ=255)                                          cAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACcac                                                                                                                                                                                 <  1:585233/250‑1 (MQ=255)                                              aCTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAac                                                                                                                                                                            <  1:795971/251‑1 (MQ=255)                                              aCTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAac                                                                                                                                                                            <  1:502760/251‑1 (MQ=255)                                                 cGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAAcaca                                                                                                                                                                         >  1:723272/1‑251 (MQ=255)                                                  gCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   >  1:277080/1‑240 (MQ=255)                                                   cTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAAcaca                                                                                                                                                                         <  1:168568/249‑1 (MQ=255)                                                   cTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACAtt                                                                                                                                                                       <  1:851738/251‑1 (MQ=255)                                                   cTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACAtt                                                                                                                                                                       <  1:779650/251‑1 (MQ=255)                                                     cAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTAt                                                                                                                                                                     <  1:273050/251‑1 (MQ=255)                                                      aaaaTCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATc                                                                                                                                                                    <  1:637643/251‑1 (MQ=255)                                                       aaaTCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACAcc                                                                                                                                                                                          >  1:125321/1‑228 (MQ=255)                                                       aaaTCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATcc                                                                                                                                                                   <  1:618389/251‑1 (MQ=255)                                                       aaaTCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATcc                                                                                                                                                                   <  1:132413/251‑1 (MQ=255)                                                        aaTCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCa                                                                                                                                                                  <  1:637605/251‑1 (MQ=255)                                                            aaCCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATtta                                                                                                                                                              <  1:728701/251‑1 (MQ=255)                                                             aCCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATttat                                                                                                                                                             <  1:498817/251‑1 (MQ=255)                                                               cGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGCTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATttatta                                                                                                                                                           <  1:18694/251‑1 (MQ=255)                                                                   cTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATg                                                                                                                                                       <  1:3031/251‑1 (MQ=255)                                                                   cTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGCGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATg                                                                                                                                                       >  1:489277/1‑251 (MQ=255)                                                                    tttGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGt                                                                                                                                                      <  1:237885/251‑1 (MQ=255)                                                                         tttCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGc                                                                                                                                                 <  1:846564/251‑1 (MQ=255)                                                                         tttCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGc                                                                                                                                                 <  1:850794/251‑1 (MQ=255)                                                                           tCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCg                                                                                                                                               <  1:156513/251‑1 (MQ=255)                                                                           tCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCg                                                                                                                                               <  1:410935/251‑1 (MQ=255)                                                                              cgTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAAt                                                                                                                                            <  1:679902/251‑1 (MQ=255)                                                                              cgTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAAt                                                                                                                                            >  1:253155/1‑251 (MQ=255)                                                                                ttAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATgg                                                                                                                                          <  1:588725/251‑1 (MQ=255)                                                                                  aaGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   >  1:597492/1‑208 (MQ=255)                                                                                    gCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTa                                                                                                                                      >  1:761394/1‑251 (MQ=255)                                                                                              cgGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACcac                                                                                                                                                                                 <  1:509646/198‑1 (MQ=255)                                                                                                   gtTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGTCAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGa                                                                                                                                                                              >  1:522220/1‑196 (MQ=255)                                                                                                   gtTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGc                                                                                                                                                 <  1:161189/225‑1 (MQ=255)                                                                                                   gtTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGa                                                                                                                        <  1:201998/250‑1 (MQ=255)                                                                                                    ttCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGAtg                                                                                                                      <  1:763891/251‑1 (MQ=255)                                                                                                       cATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATgtgt                                                                                                                   <  1:45286/251‑1 (MQ=255)                                                                                                          ccGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAAcaca                                                                                                                                                                         >  1:773020/1‑194 (MQ=255)                                                                                                               aCGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACGCCATTAACCACAGAACACATTATCCa                                                                                                                                                                  >  1:449900/1‑196 (MQ=255)                                                                                                                          cgcgAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCa                                                                                                                                                                  >  1:375729/1‑185 (MQ=255)                                                                                                                                gCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   >  1:137793/1‑162 (MQ=255)                                                                                                                                gCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGt                                                                                                                                                      >  1:333483/1‑191 (MQ=255)                                                                                                                                          ccTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAAcc                                                                                                                                                                                   >  1:239710/1‑152 (MQ=255)                                                                                                                                           cTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCg                                                                               >  1:786806/1‑251 (MQ=255)                                                                                                                                                 cTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATcc                                                                                                                                                                   >  1:98689/1‑161 (MQ=255)                                                                                                                                                 cTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATCTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAatat                                                                                                         >  1:815188/1‑219 (MQ=255)                                                                                                                                                     aaCGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAAc                                                                     <  1:113817/251‑1 (MQ=255)                                                                                                                                                            aTGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAAc                                                                     >  1:468624/1‑244 (MQ=255)                                                                                                                                                              gTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCacac                                                                                                                                                                                           <  1:506162/124‑1 (MQ=255)                                                                                                                                                                    aGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGaa                                                                                                              <  1:89339/195‑1 (MQ=255)                                                                                                                                                                     ggCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCg                                                                                                                                               >  1:753362/1‑161 (MQ=255)                                                                                                                                                                     ggCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAAt                                                           >  1:469813/1‑245 (MQ=255)                                                                                                                                                                                   gTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGAtt                                                     >  1:889263/1‑237 (MQ=255)                                                                                                                                                                                                gCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGcgctcgct                           <  1:449959/250‑1 (MQ=255)                                                                                                                                                                                                     ttATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGaa                                                                                                              >  1:273915/1‑162 (MQ=255)                                                                                                                                                                                                      tATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGAc                    <  1:278150/251‑1 (MQ=255)                                                                                                                                                                                                        ttGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAg                  <  1:422066/251‑1 (MQ=255)                                                                                                                                                                                                         tGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGc                 <  1:188887/251‑1 (MQ=255)                                                                                                                                                                                                                cGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGCGGCAAAg          <  1:393921/251‑1 (MQ=255)                                                                                                                                                                                                                 gAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGt                                                                                                                                                      <  1:187824/110‑1 (MQ=255)                                                                                                                                                                                                                   aTTGGCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGCGGCAAAGAat       <  1:238571/251‑1 (MQ=255)                                                                                                                                                                                                                      ggCATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAg                                     >  1:787359/1‑218 (MQ=255)                                                                                                                                                                                                                        cATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGCGGCAAAGAATATGtt  <  1:271990/251‑1 (MQ=255)                                                                                                                                                                                                                        cATTAATTGAACCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGCGGCAAAGAATATGtt  >  1:104074/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaCCGAATCTTTCTTTCGAATTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTCGAAAc                                                                     >  1:433915/1‑175 (MQ=255)                                                                                                                                                                                                                                                   aaTTGCAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACcac                                                                                                                                                                                 >  1:354987/1‑49 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            CGGACGCGCCAATGCCATCAAATTGCTGGCTCGCTTCCTGGCAAAACTCGCTCAAAATCAACCGCACTTTGATTTCGCGTTAAGCGATATTCGCGGTGGTTCCATCCGCAACGCCATTCCGCGCGAAGCCTTTGCCACCTTAAACTTCAACGGTGATGTTGACAGGCTGAAAAGTGCGGTGGAAAATTTCGGCGTTTTATTGAAGACCGAATTGGCATTAATTGAACCGAATCTTTCTTTCGAATTACAACCGATTGATAAACCGACAAAGATTTTCACACCATTAACCACAGAACACATTATCCATTTATTAAATGTATTGCCGAATGGCGTAATTCGCAACAGCGATGTGTTGGAAAATATTGTGGAAACTTCGCTTAGCGTCGGCGTGTTAGAAACCGAAGAAAATTGGATTAAAGCCACCATTTTAGTGCGCTCGCTGATTGACAGCGGCAAAGAATATGTT  >  1/833286‑833751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑