BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	825,664	T→C	intergenic (‑4987/+23917)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	825,664	0	T	C	99.2%	327.4 / NA	119	intergenic (‑4987/+23917)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (62/56); minor base A (0/1); total (62/57)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GTACTTTTTCGCGCAAGCCGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGTCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGTCATGACTTTTTCCGGCGATAAATACAATAATTTTAAT  >  1/825420‑825910

gTACTTTTTCGCGCAAGCCGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGgaga                                                                                                                                                                                                                                                  >  1:264417/1‑251 (MQ=255)

          gcgcAAGCCGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGa                                                                                                                                                                                                                                        >  1:392799/1‑251 (MQ=255)

                gCCGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCtt                                                                                                                                                                                                                                  >  1:647471/1‑251 (MQ=255)

                gCCGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTACACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCtt                                                                                                                                                                                                                                  >  1:52617/1‑251 (MQ=255)

                  cGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCttgt                                                                                                                                                                                                                                <  1:562593/251‑1 (MQ=255)

                   gTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCttgtt                                                                                                                                                                                                                               >  1:411008/1‑251 (MQ=255)

                       tCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTgtgt                                                                                                                                                                                                                           <  1:537905/251‑1 (MQ=255)

                             ccGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGt                                                                                                                                                                                                                        >  1:635279/1‑248 (MQ=255)

                             ccGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCt                                                                                                                                                                                                                      <  1:323878/250‑1 (MQ=255)

                              cGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGa                                                                                                                                                                                                                    <  1:693865/251‑1 (MQ=255)

                                gCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGAt                                                                                                                                                                                                                   >  1:421427/1‑250 (MQ=255)

                                    gATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTtgtg                                                                                                                                                                                                                            <  1:598371/237‑1 (MQ=255)

                                     aTACGCCGCCGCCCGCCCGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCGCTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATg                                                                                                                                                                                                                                      <  1:706008/226‑1 (MQ=255)

                                           cgccgccCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:281779/251‑1 (MQ=255)

                                           cgccgccCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:355415/251‑1 (MQ=255)

                                           cgccgccCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:475023/251‑1 (MQ=255)

                                           cgccgccCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:168083/251‑1 (MQ=255)

                                           cgccgccCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:144787/251‑1 (MQ=255)

                                                    cACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCa                                                                                                                                                                                              <  1:789676/251‑1 (MQ=255)

                                                        ccTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTgtgt                                                                                                                                                                                                                           <  1:187550/218‑1 (MQ=255)

                                                        ccTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGAt                                                                                                                                                                                          >  1:411357/1‑251 (MQ=255)

                                                        ccTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGAt                                                                                                                                                                                          >  1:856646/1‑251 (MQ=255)

                                                        ccTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGAGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGAGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGAt                                                                                                                                                                                          >  1:27043/1‑251 (MQ=255)

                                                           ttgttgTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCgg                                                                                                                                                                                       >  1:346810/1‑251 (MQ=255)

                                                           ttgttgTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCgg                                                                                                                                                                                       >  1:245762/1‑251 (MQ=255)

                                                             gttgtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGc                                                                                                                                                                                      >  1:102135/1‑250 (MQ=255)

                                                             gttgtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCa                                                                                                                                                                                     >  1:66991/1‑251 (MQ=255)

                                                                gtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTgtgt                                                                                                                                                                                                                           >  1:288715/1‑210 (MQ=255)

                                                                gtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAg                                                                                                                                                                                                          >  1:545193/1‑227 (MQ=255)

                                                                gtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGt                                                                                                                                                                                  >  1:193082/1‑251 (MQ=255)

                                                                gtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGt                                                                                                                                                                                  >  1:814324/1‑251 (MQ=255)

                                                                gtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGt                                                                                                                                                                                  >  1:633889/1‑251 (MQ=255)

                                                                gtACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCAGTCGATTTAGCCGGAGATTTTGCAGGATGTCttgttg                                                                                                                                                                                                                              >  1:433870/1‑207 (MQ=255)

                                                                       gTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGaa                                                                                                                                                                            <  1:849830/250‑1 (MQ=255)

                                                                         ttCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGc                                                                                                                                                                         <  1:679290/251‑1 (MQ=255)

                                                                           ccGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCa                                                                                                                                                                                                                                           >  1:250233/1‑183 (MQ=255)

                                                                              cgATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGcc                                                                                                                                                                     <  1:390301/250‑1 (MQ=255)

                                                                                aTACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGa                                                                                                                                                                                                                    <  1:454781/201‑1 (MQ=255)

                                                                                       ccACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAgccg                                                                                                                                                           >  1:519799/1‑251 (MQ=255)

                                                                                         aCTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTgtgt                                                                                                                                                                                                                           <  1:782004/185‑1 (MQ=255)

                                                                                          cTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATc                                                                                                                                                                                                                  <  1:31585/193‑1 (MQ=255)

                                                                                            ttATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:247173/202‑1 (MQ=255)

                                                                                              aTTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCttgttg                                                                                                                                                                                                                              <  1:308656/177‑1 (MQ=255)

                                                                                               ttGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTg                                                                                                                                                   <  1:794005/251‑1 (MQ=255)

                                                                                                     cTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGtat                                                                                                                                              <  1:222185/250‑1 (MQ=255)

                                                                                                       gTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTgtgt                                                                                                                                                                                                                           <  1:244986/171‑1 (MQ=255)

                                                                                                       gTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGtat                                                                                                                                              >  1:394997/1‑248 (MQ=255)

                                                                                                       gTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATAtt                                                                                                                                           >  1:683214/1‑251 (MQ=255)

                                                                                                       gTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATAtt                                                                                                                                           >  1:132726/1‑251 (MQ=255)

                                                                                                        ttGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATAtt                                                                                                                                           <  1:709796/250‑1 (MQ=255)

                                                                                                          gCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCttgttg                                                                                                                                                                                                                              <  1:791163/165‑1 (MQ=255)

                                                                                                          gCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       >  1:847396/1‑188 (MQ=255)

                                                                                                          gCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAATCTGATc                                                                                                                                                                                                                  >  1:424967/1‑177 (MQ=255)

                                                                                                             aTACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGa                                                                                                                                      >  1:336102/1‑250 (MQ=255)

                                                                                                             aTACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGAc                                                                                                                                     <  1:821897/251‑1 (MQ=255)

                                                                                                             aTACACAATGCCGCTATTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGCCTTGTTGTGTAGTCTGATCCGCTGAAGCAGTGAGCGCCATGATCGTCACGTTCGGAAAACTGCCTTTTTAgcctgtatcttatatacatctccgag                                                                                                                                     >  1:182361/1‑228 (MQ=255)

                                                                                                               acacAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCAt                                                                                                                                                                                             >  1:81014/1‑193 (MQ=255)

                                                                                                               acacAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTc                                                                                                                                          <  1:658973/244‑1 (MQ=255)

                                                                                                               acacAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACgg                                                                                                                                   >  1:591266/1‑251 (MQ=255)

                                                                                                                  caATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAgcg                                                                                                                                                                                                 >  1:393993/1‑186 (MQ=255)

                                                                                                                    aTGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:805466/178‑1 (MQ=255)

                                                                                                                    aTGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAAt                                                                                                                               >  1:115308/1‑250 (MQ=255)

                                                                                                                                    tttGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGaa                                                                                                              >  1:823694/1‑251 (MQ=255)

                                                                                                                                        cgcCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGc                                                                                                          <  1:274950/251‑1 (MQ=255)

                                                                                                                                                  aaaCGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTc                                                                                                                                          <  1:523313/209‑1 (MQ=255)

                                                                                                                                                     cGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCAc                                                                                                                                                                                    <  1:642776/164‑1 (MQ=255)

                                                                                                                                                        cAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCaa                                                                                           <  1:245324/250‑1 (MQ=255)

                                                                                                                                                          aaGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTg                                                                                                                                                                                                     >  1:874842/1‑142 (MQ=255)

                                                                                                                                                          aaGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATgg                                                                                        <  1:832561/251‑1 (MQ=255)

                                                                                                                                                           aGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGa                                                                                                               <  1:31511/227‑1 (MQ=255)

                                                                                                                                                               gTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTgtgt                                                                                                                                                                                                                           <  1:644316/115‑1 (MQ=255)

                                                                                                                                                                       ggTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTg                                                                                                                                                   >  1:491161/1‑179 (MQ=255)

                                                                                                                                                                       ggTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTtc                                                                                                <  1:248599/230‑1 (MQ=255)

                                                                                                                                                                         tttGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACt                                                                          <  1:340154/250‑1 (MQ=255)

                                                                                                                                                                             aaTTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCa                                                                     <  1:128691/251‑1 (MQ=255)

                                                                                                                                                                                         aaGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGaaataaa                                                         >  1:860657/1‑251 (MQ=255)

                                                                                                                                                                                          aGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCttgttg                                                                                                                                                                                                                              <  1:865791/85‑1 (MQ=255)

                                                                                                                                                                                          aGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGaaa                                                                                                             <  1:863830/198‑1 (MQ=255)

                                                                                                                                                                                           ggTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAg                                                                                                                                                                                                       <  1:457809/107‑1 (MQ=255)

                                                                                                                                                                                           ggTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGa                                                                                                               >  1:490343/1‑195 (MQ=255)

                                                                                                                                                                                           ggTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAAtt                                                       >  1:118007/1‑251 (MQ=255)

                                                                                                                                                                                           ggTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAAtt                                                       >  1:178679/1‑251 (MQ=255)

                                                                                                                                                                                           ggTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAAtt                                                       >  1:88148/1‑251 (MQ=255)

                                                                                                                                                                                            gtgtAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCtt                                                                                                 <  1:309417/208‑1 (MQ=255)

                                                                                                                                                                                            gtgtAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGaaataaat                                                        <  1:128603/249‑1 (MQ=255)

                                                                                                                                                                                              gtAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGa                                                                                                               <  1:435029/192‑1 (MQ=255)

                                                                                                                                                                                              gtAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTgg                                                                       >  1:857932/1‑232 (MQ=255)

                                                                                                                                                                                                 aCGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGc                                                                                                          >  1:218903/1‑194 (MQ=255)

                                                                                                                                                                                                       gTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTggg                                                                                                                >  1:334424/1‑182 (MQ=255)

                                                                                                                                                                                                            ggCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGt                                       >  1:856178/1‑250 (MQ=255)

                                                                                                                                                                                                               gATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCAc                                                                                                                                                                                    >  1:586733/1‑106 (MQ=255)

                                                                                                                                                                                                                       cTGCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGTCATGACTTTTTc                           <  1:648750/251‑1 (MQ=255)

                                                                                                                                                                                                                         gCCCACATAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCa                                                                     <  1:79085/207‑1 (MQ=255)

                                                                                                                                                                                                                             acaTAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTcc                                                                                                                        >  1:417895/1‑152 (MQ=255)

                                                                                                                                                                                                                              caTAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGa                                                                                                                                                                                           >  1:698952/1‑84 (MQ=255)

                                                                                                                                                                                                                              caTAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTc                                                                                                                                          >  1:669941/1‑133 (MQ=255)

                                                                                                                                                                                                                              caTAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGc                                                                                                          <  1:158681/165‑1 (MQ=255)

                                                                                                                                                                                                                              caTAAAAGTGCGGTCGATTTAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGTCATGACTTTTTCCGGCGAt                    >  1:510208/1‑251 (MQ=255)

                                                                                                                                                                                                                                      tGCGGTCGATTTAGACGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGTCATGACTTTTTCCGGCGATAAATAc              <  1:699638/249‑1 (MQ=255)

                                                                                                                                                                                                                                                ttAGCCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGTCATGACTTTTTCCGGCGATAAATACAATAATTTTAAt  <  1:194010/251‑1 (MQ=255)

GTACTTTTTCGCGCAAGCCGTTTTCCATGCCGGCATGATACGCCGCCGCCCGCACGCCTTTGTTGTACAGGGTTTCCGCGATACGTTCCACTTTATTGCGGCTGTTGCAATACACAATGCCGCTCTTGCCCTTTTGCGCCAGCACGAAACGGCAAAGCTGTTCCATGGGTTTGAATTTTTCCACCAAGGTGTAACGAATGTTCGGGCGATCGAAGCTGCCCACATAAAAGTGCGGTCGATTTAGTCGGAGATTTTGCAGGATGTCTTGTTGTGTAGTCTGATCCGCTGTAGCAGTGAGCGCCATGATCGGCACGTTCGGAAAGCTGCCTTTTAAGCCGCCGAGTTGGGTATATTCGGGACGGAAATCGTGTCCCCATTGGGAAATGCAGTGCGCTTCATCAATGGCGATAAAACTCACTTGGCATAAAGAAATAAATTGGAAAAAGCTGCTGGTCATGACTTTTTCCGGCGATAAATACAATAATTTTAAT  >  1/825420‑825910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`