Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	6,336	G→A	intergenic (–/+124798)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	6,336	0	G	A	100.0%	111.0 / NA	41	intergenic (–/+124798)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/23);  total (18/23)

AGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGCCTTACTC  >  1/6162‑6578                                                                                                                                                                               |                                                                                                                                                                                                                                                   aGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATtc                                                                                                                                                                         <  1:624546/250‑1 (MQ=11)            aTGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGtttttt                                                                                                                                                             >  1:216182/1‑251 (MQ=25)                                        tGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACct                                                                                                                                 <  1:640335/251‑1 (MQ=255)                                             tatTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTg                                                                                                                            <  1:481531/251‑1 (MQ=255)                                              atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTg                                                                                                                            >  1:841884/1‑250 (MQ=255)                                              atTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTg                                                                                                                            >  1:726662/1‑250 (MQ=255)                                                            gtTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGAc                                                                                                                                                          <  1:135309/206‑1 (MQ=38)                                                            gtTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTg                                                                                                             >  1:620748/1‑251 (MQ=255)                                                             tttttACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTg                                                                                                             <  1:427299/250‑1 (MQ=255)                                                                ttACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCGCCGAATCATTGAtgt                                                                                                         <  1:755647/251‑1 (MQ=255)                                                                ttACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGAtgt                                                                                                         <  1:5179/251‑1 (MQ=255)                                                                  aCTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGAtgttg                                                                                                       <  1:99813/251‑1 (MQ=255)                                                                         cACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGaaa                                                                                                >  1:802534/1‑251 (MQ=255)                                                                          aCTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAAc                                                                                               <  1:491354/251‑1 (MQ=255)                                                                            ttATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAg                                                                                             <  1:841577/251‑1 (MQ=255)                                                                              ataCTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCg                                                                                           >  1:706401/1‑251 (MQ=255)                                                                                      aTTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGgtaagta                                                                                   <  1:359711/251‑1 (MQ=255)                                                                                             aaTTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTc                                                                             <  1:139501/250‑1 (MQ=255)                                                                                                         aaaTTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGAccc                                                                 <  1:584545/250‑1 (MQ=255)                                                                                                         aaaTTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGAccc                                                                 <  1:165060/250‑1 (MQ=255)                                                                                                               aagaaTATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAacatac                                                          <  1:865996/251‑1 (MQ=255)                                                                                                               aagaaTATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAacatac                                                          <  1:840306/251‑1 (MQ=255)                                                                                                                 gaaTATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACtttgatc                                                                                                                                              <  1:602102/165‑5 (MQ=255)                                                                                                                   atatTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTg                                                      >  1:642818/1‑251 (MQ=255)                                                                                                                          gtgtATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGc                                                >  1:71618/1‑250 (MQ=255)                                                                                                                          gtgtATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAAATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGc                                                >  1:105936/1‑250 (MQ=255)                                                                                                                            gtATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCg                                                                                                                                             <  1:665732/155‑1 (MQ=255)                                                                                                                            gtATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATAcc                                                                                                                                  >  1:56889/1‑166 (MQ=255)                                                                                                                              aTTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGt                                                                                                                                       >  1:706583/1‑159 (MQ=255)                                                                                                                               ttACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACct                                                                                                                                 >  1:430077/1‑164 (MQ=255)                                                                                                                                    aCCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAAcc                                     <  1:374175/251‑1 (MQ=255)                                                                                                                                                                 ggATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGAc                                                                   >  1:595151/1‑192 (MQ=255)                                                                                                                                                                 ggATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGc         >  1:806637/1‑250 (MQ=255)                                                                                                                                                                  gATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCa                                                                       <  1:555247/187‑1 (MQ=255)                                                                                                                                                                  gATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAacat                                                            <  1:40854/198‑1 (MQ=255)                                                                                                                                                                  gATTAAGCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGc         >  1:453880/1‑249 (MQ=255)                                                                                                                                                                        gCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACcttc                                                                                                                               >  1:88769/1‑125 (MQ=255)                                                                                                                                                                        gCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGAtg                                                                                                          <  1:301797/146‑1 (MQ=255)                                                                                                                                                                        gCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAGTTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGCCTTACTc  >  1:440912/1‑250 (MQ=255)                                                                                                                                                                        gCCCGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGCCTTACTc  >  1:236116/1‑250 (MQ=255)                                                                                                                                                                         cccGGTACAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGCCTTACTc  <  1:607234/249‑1 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                                                                   AGGGAATTGCTATGATAATGCGGTGATTGAAAGCTTTTTTGCGATATTAAAATCTGAGTGTTTTTACTCACGCACTTATACTTCGATTGCCGAATTACAGGCGGAAATTGAAGAATATTTGGTGTATTACAACCAAGAACGAATTAAACTTGATTTAAAAGGATTAAGCCCGGTGCAATACCGAGCTCAATATTTAAGTTAACTAACCTGTCCAACTTTTGGGGGGCAGATCAAAGTGCGGTCAAAATTCTCAACGTTTTTTGACCGCACTTTTAGCGTAAGGTATACCTTCTTGCCCACCGAATCATTGATGTTGTTTGAAACAGCGGGTAAGTAATTGTCGGATCATGACCCAACATACATTGCCACGCCGTCGGAAACCTGCATTTCAAACGACTTTATCGTTTTGCCTTACTC  >  1/6162‑6578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑