Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	809,728	+A	intergenic (+72606/+10565)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	809,725	1	.	A	100.0%	140.0 / NA	54	intergenic (+72603/+10568)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (35/19);  total (35/19)

TGCCTCAATAAATTGTGTTTCTTTTTTAATAGAAAATAATTTAAAGCCCACCATAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTTCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCC‑AAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAGGTGAGAACAAATGCCGATACCATTAACC  >  1/809486‑809969                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     tGCCTCAATAAATTGTGTTTCTTTTTTAATAGAAAATAATTTAAAGCCCACCATAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCttt                                                                                                                                                                                                                                            >  1:708219/1‑251 (MQ=255)                     cTTTTTTAATAGAAAATAATTTAAAGCCCACCATAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATaa                                                                                                                                                                                                                        <  1:50982/251‑1 (MQ=255)                     cTTTTTTAATAGAAAATAATTTAAAGCCCACCATAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATaa                                                                                                                                                                                                                        <  1:60235/251‑1 (MQ=255)                                  aaaTAATTTAAAGCCCACCATAAAAGAAATCATCGTGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCGGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATaa                                                                                                                                                                                                                        <  1:501113/238‑1 (MQ=255)                                  aaaTAATTTAAAGCCCACCATAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATaa                                                                                                                                                                                                                        >  1:324965/1‑238 (MQ=255)                                                   ccaTAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAAc                                                                                                                                                                                           >  1:713900/1‑250 (MQ=255)                                                      tAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATaa                                                                                                                                                                                                                        <  1:721647/218‑1 (MQ=255)                                                               taaaatcctatgttgagacAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCt                                                                                                                                                                              <  1:304975/233‑1 (MQ=255)                                                                       gATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATg                                                                                                                                                                                   <  1:380401/238‑1 (MQ=255)                                                                       gATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTg                                                                                                                                                                          >  1:394929/1‑247 (MQ=255)                                                                        atatAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCtt                                                                                                                                                                                                                             >  1:597970/1‑195 (MQ=255)                                                                        atatAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATg                                                                                                                                                                                   >  1:614464/1‑237 (MQ=255)                                                                               ggCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGc                                                                                                                                                                                                                               <  1:760414/186‑1 (MQ=255)                                                                                gCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGc                                                                                                                                                                                                     >  1:441218/1‑211 (MQ=255)                                                                                gCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGaa                                                                                                                                                               <  1:496190/249‑1 (MQ=255)                                                                                 cAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCt                                                                                                                                                                              <  1:191797/233‑1 (MQ=255)                                                                                     aGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGa                                                                                                                                                                                             <  1:514979/214‑1 (MQ=255)                                                                                                cTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTGAAAGAATTAAGCAAATAGGAACCa                                                                                                                                                                                         >  1:594505/1‑207 (MQ=255)                                                                                                cTATTACCCAGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAg                                                                                                                                              >  1:152495/1‑250 (MQ=255)                                                                                                         aGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGc                                                                                                                                                                                                     >  1:720397/1‑186 (MQ=255)                                                                                                         aGTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAg                                                                                                                                                              >  1:59900/1‑225 (MQ=255)                                                                                                          gTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCtgttg                                                                                                                                                                    <  1:315720/218‑1 (MQ=255)                                                                                                          gTCAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAaattaa                                                                                                                                   >  1:392239/1‑251 (MQ=255)                                                                                                            cAATATAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTTAAGGAACAAAGAAAACAAGTATAAaattaatt                                                                                                                                 >  1:400191/1‑251 (MQ=255)                                                                                                              atatAACCGATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGc                                                                                                                                                                         >  1:79375/1‑209 (MQ=255)                                                                                                                      gATATCGCCGTTTGCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAAc                                                                                                                                                 <  1:825100/225‑1 (MQ=255)                                                                                                                                tttgcTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAgg                                                                                                                                                                                >  1:97408/5‑184 (MQ=255)                                                                                                                                        gCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCAt                                                                                                                                                                                                                                  >  1:7071/1‑126 (MQ=255)                                                                                                                                         cTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCt                                                                                                                                                                              >  1:665246/1‑177 (MQ=255)                                                                                                                                             tGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGtt                                                                                                >  1:86945/1‑251 (MQ=255)                                                                                                                                              gATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATg                                                                                                                                                                                   >  1:796223/1‑167 (MQ=255)                                                                                                                                              gATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGttt                                                                                               >  1:544378/1‑251 (MQ=255)                                                                                                                                                          ggAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAAc                                                                                                                                                                                           >  1:603716/1‑147 (MQ=255)                                                                                                                                                                        cagcagTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATAc                                                                                                                            <  1:336121/196‑1 (MQ=255)                                                                                                                                                                           cagTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATaa                                                                                                                                                                                                                        >  1:224653/1‑101 (MQ=255)                                                                                                                                                                           cagTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCa                                                                  >  1:891339/1‑251 (MQ=255)                                                                                                                                                                             gTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCtgttg                                                                                                                                                                    <  1:177074/151‑1 (MQ=255)                                                                                                                                                                                              cGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAgg                                                                                                                                                             <  1:202703/141‑1 (MQ=255)                                                                                                                                                                                                           gCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAg                                                                                                                                              >  1:682475/1‑143 (MQ=255)                                                                                                                                                                                                           gCATCGGCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAg                                   >  1:598593/1‑250 (MQ=255)                                                                                                                                                                                                                ggCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAgg                             >  1:729878/1‑251 (MQ=255)                                                                                                                                                                                                                ggCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGAAATTCGATTTGATAATAACCGAgg                             >  1:179680/1‑251 (MQ=255)                                                                                                                                                                                                                 gCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTttctt                                                                                                       >  1:513979/1‑176 (MQ=255)                                                                                                                                                                                                                 gCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAg                                                                      >  1:444122/1‑209 (MQ=255)                                                                                                                                                                                                                 gCCTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGCAATTCGATTTGATCATAGCCGAggg                            >  1:366151/1‑250 (MQ=255)                                                                                                                                                                                                                  ccTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGCTATTCGATTTGATCATAGCCGAGGTg                           >  1:756534/1‑251 (MQ=255)                                                                                                                                                                                                                   cTGTACACCTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAaat                                                                                                                                      >  1:809705/1‑143 (MQ=255)                                                                                                                                                                                                                          ccTTGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTTATCATAGCCGAGGGGAGAACaaa                   >  1:620444/1‑251 (MQ=255)                                                                                                                                                                                                                            ttGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGc                                                                                                             >  1:477677/1‑159 (MQ=255)                                                                                                                                                                                                                             tGTAACATCATCAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAGGTGAGAACAAATGc                <  1:225912/251‑1 (MQ=255)                                                                                                                                                                                                                                   atcatcAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATg                                                                                                                                                                                   <  1:153941/82‑1 (MQ=255)                                                                                                                                                                                                                                   atcatcAATCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAGGTGAGAACAAATGCCGATa           >  1:9722/1‑250 (MQ=255)                                                                                                                                                                                                                                           tCGTCCAAAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAGGTGAGAACAAATGCCGATACCATTAAcc  <  1:889441/251‑1 (MQ=255)                                                                                                                                                                                                                                           tCGTCCAAAATTGCTTTCACCTGGCATGGCCTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAGGTGAGAACAAATGCCGATACCATTAAcc  <  1:25831/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     TGCCTCAATAAATTGTGTTTCTTTTTTAATAGAAAATAATTTAAAGCCCACCATAAAAGAAATCATCATGGATATAAAGGCAATAGGGAAGGAGGCTATTACCCAGTCAATATAACCGATATCGCCGTTTTCTTGGCTATTGATAAAACCAACGGAAATAATGTTGGCAGCAGTACCTGTCATCACACCCGATGTGGCAATCGCATCGGCCTGTACACCTTGTAACATCATCAATCGTCC‑AAATTGCTTTCACCTGGCATGGCTTTATAAATTTCTAAAAGAATTAAGCAAATAGGAACCATTAATGAGGCTCGTGCTGTTGTTGAAGGAACAAAGAAAACAAGTATAAAATTAATTAATACAAGCACAAATAAGGCTTTCTTCGGTGTTTGTCCAAAATGAGTAATCATCCAAAGCGCAAAACGGCGGGCGATATTCGATTTGATCATAGCCGAGGTGAGAACAAATGCCGATACCATTAACC  >  1/809486‑809969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑