Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 97,407 +GG intergenic (–/+33727) – / ← unknown –/tRNA‑Ala‑GGC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*197,4071.G98.6% 191.2 / ‑4.8 72intergenic (–/+33727)–/unknown–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (26/45);  minor base A (1/0);  total (27/45)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
*197,4072.G100.0% 198.3 / NA 72intergenic (–/+33727)–/unknown–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (27/45);  total (27/45)

GCCGGTATTAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGAC‑‑CGTGCTGCAGGTGCAAAACACCATTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGG  >  1/97161‑97644
                                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                             
gCCGGTATTAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGAcggcg                                                                                                                                                                                                                                             >  1:337808/1‑247 (MQ=255)
    gTATTAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGAcggcgtg                                                                                                                                                                                                                                           >  1:890303/1‑243 (MQ=255)
    gTATTAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGAcggcgtg                                                                                                                                                                                                                                           >  1:341225/1‑243 (MQ=255)
    gTATTAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGAAATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGAcggcgtgcg                                                                                                                                                                                                                                         >  1:493741/1‑243 (MQ=255)
        tAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGATCAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAg                                                                                                                                                                                                                                     <  1:43306/251‑1 (MQ=255)
        tAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAg                                                                                                                                                                                                                                     <  1:297052/251‑1 (MQ=255)
                  ccAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGTACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAac                                                                                                                                                                                                                            <  1:430899/250‑1 (MQ=255)
                  ccAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAac                                                                                                                                                                                                                            <  1:534341/250‑1 (MQ=255)
                  ccAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAac                                                                                                                                                                                                                            <  1:590012/250‑1 (MQ=255)
                  ccAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAac                                                                                                                                                                                                                            <  1:232405/250‑1 (MQ=255)
                   cAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAacac                                                                                                                                                                                                                          <  1:131191/251‑1 (MQ=255)
                   cAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAacac                                                                                                                                                                                                                          <  1:610792/251‑1 (MQ=255)
                                ccGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:398741/251‑1 (MQ=255)
                                ccGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:691023/251‑1 (MQ=255)
                                ccGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:867979/251‑1 (MQ=255)
                                ccGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:828923/251‑1 (MQ=255)
                                     ttGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGc                                                                                                                                                                                                        <  1:720149/251‑1 (MQ=255)
                                     ttGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGc                                                                                                                                                                                                        <  1:361302/251‑1 (MQ=255)
                                            aTTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:223848/239‑1 (MQ=255)
                                            aTTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             >  1:752624/1‑239 (MQ=255)
                                            aTTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGa                                                                                                                                                                                                 >  1:205118/1‑251 (MQ=255)
                                            aTTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGa                                                                                                                                                                                                 >  1:524904/1‑251 (MQ=255)
                                                                                  ccGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGatat                                                                                                                                                           <  1:14536/251‑1 (MQ=255)
                                                                                  ccGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGatat                                                                                                                                                           <  1:872799/251‑1 (MQ=255)
                                                                                     ctctTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCAt                                                                                                                                                                                                           >  1:856152/1‑200 (MQ=255)
                                                                                     ctctTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAg                                                                                                                                                        >  1:42089/1‑251 (MQ=255)
                                                                                       ctTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCAt                                                                                                                                                                                                           <  1:269626/198‑1 (MQ=255)
                                                                                       ctTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCg                                                                                                                                                      >  1:108278/1‑251 (MQ=255)
                                                                                       ctTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCg                                                                                                                                                      <  1:533896/251‑1 (MQ=255)
                                                                                       ctTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACAGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAg                                                                                                                                                        >  1:30788/1‑249 (MQ=255)
                                                                                            aTTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAAcc                                                                                                                                                 <  1:207211/251‑1 (MQ=255)
                                                                                                cGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGcc                                                                                                                                             <  1:656031/251‑1 (MQ=255)
                                                                                                     tGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCg                                                                                                                                        <  1:296764/251‑1 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAac                                                                                                                                                                                                                            >  1:95095/1‑166 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGat                                                                                                                                                             <  1:861044/229‑1 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGat                                                                                                                                                             <  1:820412/229‑1 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCg                                                                                                                                        >  1:421215/1‑250 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCg                                                                                                                                        >  1:189630/1‑250 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCg                                                                                                                                        >  1:652414/1‑250 (MQ=255)
                                                                                                      gTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTAACCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCg                                                                                                                                        >  1:523999/1‑250 (MQ=255)
                                                                                                        cacaGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:165094/179‑1 (MQ=255)
                                                                                                        cacaGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCaa                                                                                                                                     >  1:140340/1‑251 (MQ=255)
                                                                                                            ggAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATc                                                                                                                                                                                                              <  1:154367/174‑1 (MQ=255)
                                                                                                            ggAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCg                                                                                                                                                                                      <  1:190687/198‑1 (MQ=255)
                                                                                                             gAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAga                                                                                                                                   <  1:693536/248‑1 (MQ=255)
                                                                                                                 aGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:837859/170‑1 (MQ=255)
                                                                                                                      acacAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCa                                                                                                                       >  1:452116/1‑251 (MQ=255)
                                                                                                                            ccGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAg                                                                                                                                                        >  1:289217/1‑212 (MQ=255)
                                                                                                                            ccGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAgac                                                                                                                                  >  1:322521/1‑234 (MQ=255)
                                                                                                                               tATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGCCGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAac                                                                                                              <  1:78878/251‑1 (MQ=255)
                                                                                                                                aTGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAacg                                                                                                             >  1:294002/1‑251 (MQ=255)
                                                                                                                                     gAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAg                                                                                                                                                        <  1:723226/203‑1 (MQ=255)
                                                                                                                                        ccGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTc                                                                                                     <  1:19666/251‑1 (MQ=255)
                                                                                                                                         cGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGCTCCCTTATcc                                                                                                                                                                                                             >  1:798327/1‑146 (MQ=255)
                                                                                                                                                      gTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGAtt                                                                                       >  1:250702/1‑251 (MQ=255)
                                                                                                                                                      gTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGAtt                                                                                       >  1:692000/1‑251 (MQ=255)
                                                                                                                                                                 tttttGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACACTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTcc                                                                            <  1:384147/251‑1 (MQ=255)
                                                                                                                                                                     tGAAAGCGCGACGGCACAAAAAGCCTCCGTTATTCATCTGGAACCGCAAGCAGACTGCCTGCAACCCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCAccc                                                                        <  1:507974/251‑1 (MQ=255)
                                                                                                                                                                     tGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCAccc                                                                        <  1:894300/251‑1 (MQ=255)
                                                                                                                                                                                   cacaAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTc                                                          <  1:792854/251‑1 (MQ=255)
                                                                                                                                                                                            ccTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGaaaaa                                                 >  1:544826/1‑251 (MQ=255)
                                                                                                                                                                                             cTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTtct                                                                                                                                                                                        <  1:7005/115‑1 (MQ=255)
                                                                                                                                                                                                  atatTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGt                                            >  1:852864/1‑250 (MQ=255)
                                                                                                                                                                                                   tatTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAga                                                                                                                                   >  1:879791/1‑162 (MQ=255)
                                                                                                                                                                                                    atTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATcc                                                                                                                                                                                                             <  1:554505/87‑1 (MQ=255)
                                                                                                                                                                                                       cATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCg                                      <  1:678000/251‑1 (MQ=255)
                                                                                                                                                                                                        aTCTGGACCCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGt                                     <  1:418473/251‑1 (MQ=255)
                                                                                                                                                                                                        aTCTGGAACCGCAAGCAGACTGCCTGCAATCCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACCCCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGt                                     <  1:573150/251‑1 (MQ=255)
                                                                                                                                                                                                        aTCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTc                                                          <  1:485121/230‑1 (MQ=255)
                                                                                                                                                                                                         tCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGtt                                    >  1:801912/1‑251 (MQ=255)
                                                                                                                                                                                                                    aaGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAAt                         <  1:285935/251‑1 (MQ=255)
                                                                                                                                                                                                                     aGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAAt                         >  1:874976/1‑250 (MQ=255)
                                                                                                                                                                                                                       cAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAg                                                >  1:177051/1‑225 (MQ=255)
                                                                                                                                                                                                                               ctgcAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAAt                         >  1:749395/1‑240 (MQ=255)
                                                                                                                                                                                                                                  cAAACCCGTTTTCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAAc            <  1:844724/250‑1 (MQ=255)
                                                                                                                                                                                                                                           tttCGCATTGACGGCGTGCTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCgg  <  1:238877/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                             
GCCGGTATTAATTGAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTATTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCGCGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGAC‑‑CGTGCTGCAGGTGCAAAACACCATTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATATTAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCACCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGG  >  1/97161‑97644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑