Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	704,728	+T	intergenic (+439/‑10354)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Pro‑TGG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	704,726	1	.	T	100.0%	131.2 / NA	52	intergenic (+437/‑10356)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Pro‑TGG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (22/30);  total (22/30)

AAAGAACGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTG‑TTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCATTTAGGAATGGTATTTACTCTGGG  >  1/704497‑704907                                                                                                                                                                                                                                       |                                                                                                                                                                                      aaaGAACGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGtat                                                                                                                                                                    <  1:64173/250‑1 (MQ=17) aaaGAACGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGtat                                                                                                                                                                    <  1:310331/250‑1 (MQ=17)     aaCGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGc                                                                                                                                                                 <  1:215043/249‑1 (MQ=21)     aaCGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGc                                                                                                                                                                 <  1:658941/249‑1 (MQ=21)     aaCGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGAGAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTc                                                                                                                                                               <  1:437185/251‑1 (MQ=21)          gagaAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAgaga                                                                                                                                                          >  1:874561/1‑251 (MQ=37)                         gAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAAc                                                                                                                                                                        >  1:596511/1‑222 (MQ=14)                         gAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAAt                                                                                                                                                  <  1:861181/244‑1 (MQ=255)                                aaagcgaaagcgCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTAc                                                                                                                                    <  1:862751/251‑1 (MQ=255)                                                 cagagagaGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGa                                                                                                                   >  1:126365/3‑251 (MQ=255)                                                  ggAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTc                                                                                                                                                               <  1:639473/206‑1 (MQ=25)                                                      agagGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATGCCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGataata                                                                                                                                                     <  1:190116/212‑1 (MQ=255)                                                         ggATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTAc                                                                                                                                    <  1:356047/226‑1 (MQ=255)                                                                       gAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAgaga                                                                                                                                                          <  1:554253/190‑1 (MQ=255)                                                                         atatTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGt                                                                                           <  1:604548/251‑1 (MQ=255)                                                                               gcggcgATAGTGCGGTGGTACCACCTGAAACCATGCCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGAc                                                                                      <  1:131053/250‑1 (MQ=255)                                                                                     aTAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGtat                                                                                                                                                                    <  1:546963/166‑1 (MQ=21)                                                                                        gTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTaa                                                                                 <  1:393878/246‑1 (MQ=255)                                                                                        gTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTc                                                                             >  1:793347/1‑250 (MQ=255)                                                                                             gtggtACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCa                                                                                                        >  1:882247/1‑218 (MQ=255)                                                                                               ggtACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACtt                                                                                                                                  <  1:160177/190‑1 (MQ=255)                                                                                               ggtACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGa                                                                                       >  1:262240/1‑233 (MQ=255)                                                                                               ggtACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAACGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTAc                                                                                                                                    <  1:49533/188‑1 (MQ=255)                                                                                                 tACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCATAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAggg                                                                   <  1:835662/251‑1 (MQ=255)                                                                                                     accTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTttatta                                                                                                                             <  1:727822/189‑1 (MQ=255)                                                                                                     accTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAg                                                                                                      >  1:34320/1‑212 (MQ=255)                                                                                                     accTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGtcat                                                               >  1:297241/1‑251 (MQ=255)                                                                                                     accTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGtcat                                                               >  1:828490/1‑251 (MQ=255)                                                                                                      ccTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAggg                                                                   <  1:52394/246‑1 (MQ=255)                                                                                                      ccTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGtcat                                                               >  1:543302/1‑250 (MQ=255)                                                                                                      ccTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGacat                                                               >  1:864086/1‑250 (MQ=255)                                                                                                            aCCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAg                                                         >  1:173882/1‑250 (MQ=255)                                                                                                             ccATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCg                                                       <  1:135433/251‑1 (MQ=255)                                                                                                             ccATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCg                                                       <  1:698282/251‑1 (MQ=255)                                                                                                               aTACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGAt                                                     >  1:534188/1‑251 (MQ=255)                                                                                                                tACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATc                                                    <  1:543224/251‑1 (MQ=255)                                                                                                                            aaGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAgg                                          <  1:875140/249‑1 (MQ=255)                                                                                                                              gTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGatat                                      >  1:505821/1‑251 (MQ=255)                                                                                                                                    ggCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAAt                                                                               >  1:515120/1‑204 (MQ=255)                                                                                                                                           aTGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTaa                                                                                         >  1:826955/1‑187 (MQ=255)                                                                                                                                                  tGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCAttttacgg                  <  1:410561/251‑6 (MQ=255)                                                                                                                                                    gTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAgg                                          <  1:580333/225‑1 (MQ=255)                                                                                                                                                    gTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCAttttacggta                >  1:739448/1‑244 (MQ=255)                                                                                                                                                      aGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTtta                                                                                                                                <  1:631918/137‑1 (MQ=255)                                                                                                                                                       gtgtGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGAt                                                     <  1:655442/211‑1 (MQ=255)                                                                                                                                                            gggCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCAttttacggt                 >  1:14958/1‑236 (MQ=255)                                                                                                                                                                aTCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTc                                                                                                                                                               >  1:296779/1‑96 (MQ=255)                                                                                                                                                                    ccATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGAc                                                 >  1:253371/1‑202 (MQ=255)                                                                                                                                                                     cATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGCACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAg                                                         >  1:71476/1‑193 (MQ=255)                                                                                                                                                                              gTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCa                                                                                                        <  1:592874/137‑1 (MQ=255)                                                                                                                                                                                      ccGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCAttttacggtatcgtcggtcaggag  <  1:493680/231‑22 (MQ=39)                                                                                                                                                                                                         gCCCTGATGTGAGAGCATCGGGGCTTTTTGTTTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCAttttacggtatcgtcggtcaggag  >  1:158135/1‑191 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                      AAAGAACGAGAGAAGCGAAGAGCGGAATAAGAAAGCGAAAGCGCGGAAAGGAGAGAGGATAGTCATCAAAGAATATTGGCGGCGATAGTGCGGTGGTACCACCTGAAACCATACCGAACTCAGAAGTGAAAGGCCGTAATGCCGATGGTAGTGTGGGGCATCCCCATGTGAGAGTAGGTCACCGCCAAGCCTGATAGGGAGCCCTGATGTGAGAGCATCGGGGCTTTTTG‑TTATAGCTAGACAACGTATAGCTCAGAGATAATAAATTCAGACACAGGTACTTTATTATGAACTTGGATTGTAAATTCAAGCCCATAAAAGTAAGACATTAAATTCCGAATTAGGGTCATCACTAGCGATCGACCAAAAGGATATTTAAAAATTGCATTTAGGAATGGTATTTACTCTGGG  >  1/704497‑704907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑