Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 534,064 (T)5→6 intergenic (+36511/‑165279) unknown → / → unknown tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1534,0591.T91.9% 232.2 / 14.6 86intergenic (+36506/‑165284)unknown/unknowntRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base . (7/0);  new base T (8/71);  total (15/71)
Fisher's exact test for biased strand distribution p-value = 1.20e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTCACACCGTCTCATCCTGAGTTATATAATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTG‑TTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTGTACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGTCGATCAACATATACCAACCCGGCCCC  >  1/533822‑534302
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
cGGTCACACCGTCTCATCCTGAGTTATATAATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGGTTGACAAAAAAAAAAAAGAACAAAACGCTCCCCTCTGCATATAAATA‑TTTT‑‑TTAAAAAA‑AT‑TA‑‑Tttttt‑ttttttttaaaaa                                                                                                                                                                                                                                          >  1:594969/1‑243 (MQ=255)
          gTCTCATCCTGAGTTATATAATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTTGCAAAAAAAAAAAGGAACATAAAGCTCCGTTCTGCCTATAAATATTTTT‑‑TAAAAAAA‑Tt‑tt‑‑tttttt‑tttttttaaaaatttacgccgg                                                                                                                                                                                                                                 >  1:592647/1‑234 (MQ=255)
               aTCCTGAGTTATATAATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCaaaaa                                                                                                                                                                                                                                            <  1:126014/235‑1 (MQ=255)
                         atataATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGGTTGTCAAAAAAAAAAAAGAACATAACACTCCCCTCTTCCCATTACTATTTTT‑‑TAAAAAAA‑TT‑AT‑‑TTTTTT‑TTTTTTTAAAATTACACGCCCGTAT‑CCCG‑AAAGGCgg                                                                                                                                                                                                                >  1:70359/1‑251 (MQ=255)
                            taATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAAAAAGGATCATAACGCTCCGTTCTGCATATAAATA‑TTTT‑‑TAAAAAAA‑AT‑TATTTTTTTT‑TTTTTTAAAAATT‑CAAGCCCGGAT‑CCGG‑AAAGACGGTaa                                                                                                                                                                                                             >  1:201366/1‑251 (MQ=255)
                                ttGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAacac                                                                                                                                                                                                         <  1:405313/251‑1 (MQ=255)
                                   aTCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAacacac                                                                                                                                                                                                       <  1:521637/250‑1 (MQ=255)
                                   aTCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACAcc                                                                                                                                                                                                      <  1:556929/251‑1 (MQ=255)
                                       gTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTTTCAAAAAAAAAAAGGATCATAACGCTCCCGTCTTCATATAACTA‑TTTT‑‑TAAAACAATTT‑TT‑‑TTTTTT‑TTTTTTTTAAATTTTACGCCCGTAT‑CCGG‑AAAGACGGGAAACACACCCGTg                                                                                                                                                                                                  >  1:715718/1‑251 (MQ=255)
                                       gTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTG‑ttt                                                                                                                                                                                                                                                    <  1:360350/202‑1 (MQ=255)
                                       gTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGGTTTACAAAAAAAAAAAGGAAAATAAAACCCCCCTCTTCCTATAACTA‑TTTT‑‑TAAAAAAA‑AT‑TATTTTTTTT‑TTTTTTAAAAATT‑CACGCCCGTAT‑CCGG‑AAAAGCGGTaaaactttctctt                                                                                                                                                                                                  >  1:653909/1‑241 (MQ=255)
                                                            aTAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTcaacaa                                                                                                                                                                              <  1:89072/250‑1 (MQ=255)
                                                                     cAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATAc                                                                                                                                                                    <  1:155292/251‑1 (MQ=255)
                                                                     cAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATAc                                                                                                                                                                    <  1:230687/251‑1 (MQ=255)
                                                                     cAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATAc                                                                                                                                                                    <  1:839669/251‑1 (MQ=255)
                                                                           cccTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACAtt                                                                                                                                                                 <  1:91693/248‑1 (MQ=255)
                                                                           cccTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAAAAAAGATCATAAAACCCCGCTCTGCATTTAACTA‑TTTTTAAAAAAAAA‑TA‑TT‑‑TTTTTT‑TTTTTTAAAAATT‑TACGCCGGCAT‑CCGG‑AAAGACGGGAAAAAAAACCGTGGGGCCGGGTTTATTTTACAAAACAACATACAttttg                                                                                                                                                              >  1:513528/1‑249 (MQ=255)
                                                                           cccTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGGTTGACAAAAAAAAAAAAGATCAAAAAACCCCCCTCTGCCTATTACTATTTTT‑‑TAAAAAAA‑TTATT‑‑TTTTTT‑TTTTTTAAAAATT‑CACGCCGGGGTCCCGG‑AAAG‑CGGTGAAAACACCCGCGGCGCCGGGTTTATTtaacaaaccaccctacctct                                                                                                                                                                >  1:805250/1‑235 (MQ=255)
                                                                             cTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGtt                                                                                                                                                            <  1:272610/251‑1 (MQ=255)
                                                                              ttAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTc                                                                                                                                                           <  1:670812/251‑1 (MQ=255)
                                                                                   ttGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATc                                                                                                                                                      <  1:549444/251‑1 (MQ=255)
                                                                                           tGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTgaaga                                                                                                                                              <  1:97526/251‑1 (MQ=255)
                                                                                            gATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGGTTTACAAAAAAAAAAAGGAACAAAAAACCCCCTTCTGCCTATAACTA‑TTTT‑‑TAAAAAAA‑AT‑TAT‑TTTTTT‑TTTTTTAAAAATTTCAAGACGGGAT‑ACGGAAAAGT‑GGGAAAAAAACCGGTGGCGGCGGGGTTATTTAAAAACACATCATACATTTTGTTtacacacgtggaaaa                                                                                                                                             >  1:388477/1‑240 (MQ=255)
                                                                                            gATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAAGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATAc                                                                                                                                                                    <  1:511636/228‑1 (MQ=255)
                                                                                             aTCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGaagaag                                                                                                                                            <  1:447231/251‑1 (MQ=255)
                                                                                             aTCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGaagaag                                                                                                                                            <  1:215111/251‑1 (MQ=255)
                                                                                                 aaTTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATAc                                                                                                                                                                    <  1:531074/223‑1 (MQ=255)
                                                                                                           cAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAAC‑A‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGtt                                                                                                                                                            <  1:421311/220‑1 (MQ=255)
                                                                                                                  tGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGc                                                                                                                       <  1:789675/251‑1 (MQ=255)
                                                                                                                        tGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGgtg                                                                                                                 <  1:157182/251‑1 (MQ=255)
                                                                                                                            aTTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATg                                                                                                             <  1:78517/251‑1 (MQ=255)
                                                                                                                             ttACAGGATTAAATTATCAGTGAGAACGGGTTATATC‑GCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:868342/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:526087/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:523378/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:630757/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:121782/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:517957/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:479273/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:721676/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:732164/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:428088/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:405457/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:362181/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:139419/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:141620/251‑1 (MQ=255)
                                                                                                                              tACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:22595/251‑1 (MQ=255)
                                                                                                                                    aTTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGt                                                                                                                                                                                                   <  1:132874/157‑1 (MQ=255)
                                                                                                                                      ttAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTc                                                                                                   <  1:731680/251‑1 (MQ=255)
                                                                                                                                            aTCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATc                                                                                                                                                      <  1:14666/194‑1 (MQ=255)
                                                                                                                                                 tGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:662785/250‑1 (MQ=255)
                                                                                                                                                 tGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:269827/250‑1 (MQ=255)
                                                                                                                                                 tGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:735972/250‑1 (MQ=255)
                                                                                                                                                 tGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCAAATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:804477/250‑1 (MQ=255)
                                                                                                                                                         ggTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:532287/224‑1 (MQ=255)
                                                                                                                                                             atatCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTc                                                                                                                                                                                   <  1:184979/148‑1 (MQ=255)
                                                                                                                                                             atatCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:609643/220‑1 (MQ=255)
                                                                                                                                                             atatCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:147523/238‑1 (MQ=255)
                                                                                                                                                                      ggTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCtt                                                                            >  1:173503/1‑242 (MQ=255)
                                                                                                                                                                       gTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTaa                                                                                                                                    <  1:206957/185‑1 (MQ=255)
                                                                                                                                                                                aaaaaGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:403076/219‑1 (MQ=255)
                                                                                                                                                                                          gATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAacac                                                                                                                                                                                                         <  1:99107/97‑1 (MQ=255)
                                                                                                                                                                                          gATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATAc                                                                                                                                                                    >  1:232995/1‑134 (MQ=255)
                                                                                                                                                                                          gATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGgtg                                                                                                         <  1:270576/193‑1 (MQ=255)
                                                                                                                                                                                          gATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGcc                                                >  1:122778/1‑250 (MQ=255)
                                                                                                                                                                                            tCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGtt                                                                                                                                                            >  1:721378/1‑140 (MQ=255)
                                                                                                                                                                                            tCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTgaag                                                                                                                                               <  1:562669/153‑1 (MQ=255)
                                                                                                                                                                                            tCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTAtagg                                                                                                           <  1:61101/189‑4 (MQ=255)
                                                                                                                                                                                            tCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAAtt                                                                               <  1:231874/217‑1 (MQ=255)
                                                                                                                                                                                               tAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:729867/186‑1 (MQ=255)
                                                                                                                                                                                                      ccGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           >  1:406584/1‑179 (MQ=255)
                                                                                                                                                                                                      ccGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:611896/197‑1 (MQ=255)
                                                                                                                                                                                                      ccGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAgg                                                                  <  1:94365/220‑1 (MQ=255)
                                                                                                                                                                                                        gTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:642301/177‑1 (MQ=255)
                                                                                                                                                                                                        gTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:655914/177‑1 (MQ=255)
                                                                                                                                                                                                        gTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:687146/177‑1 (MQ=255)
                                                                                                                                                                                                             gCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCgg                                                                                                                                                                                                    <  1:756574/83‑1 (MQ=255)
                                                                                                                                                                                                             gCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGtt                                                                                                                                                            <  1:281165/123‑1 (MQ=255)
                                                                                                                                                                                                             gCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           >  1:90851/1‑172 (MQ=255)
                                                                                                                                                                                                             gCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         <  1:854140/190‑1 (MQ=255)
                                                                                                                                                                                                              cATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGt                            <  1:262368/250‑1 (MQ=255)
                                                                                                                                                                                                               atatAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:99064/170‑1 (MQ=255)
                                                                                                                                                                                                                     cTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTAGACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGTCGATCAAc                    <  1:775637/251‑1 (MQ=255)
                                                                                                                                                                                                                       a‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGTCGATCAACat                  >  1:424557/1‑251 (MQ=255)
                                                                                                                                                                                                                                aaaaCAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGTCGATCAACATATAcc             <  1:19930/250‑1 (MQ=255)
                                                                                                                                                                                                                                    cAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:429261/152‑1 (MQ=255)
                                                                                                                                                                                                                                    cAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATAc                                                                                         >  1:140614/1‑170 (MQ=255)
                                                                                                                                                                                                                                    cAA‑TT‑TA‑‑TTTTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTATCGATCAACATATACCAAccc        <  1:441299/251‑1 (MQ=255)
                                                                                                                                                                                                                                      a‑tt‑ta‑‑tttTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATggg                                                                                                           <  1:445621/150‑1 (MQ=255)
                                                                                                                                                                                                                                            a‑‑tttTTGTTTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTATACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGTCGATCAACATATACCAACCCGGcccc  <  1:890554/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
CGGTCACACCGTCTCATCCTGAGTTATATAATTTGATCGGTTATAATCCGATAAGCGCGGATAACTTATCAACCACCCTTAATTTGTCTATTGATCAAATTCTCATTCAACTGTTGGAACTGGAATTACAGGATTTAATTATCAGTGAGAACGGGTTATATCGGCGGGTTTGACAAAAAAAGAACGGATCATAACGCTCCGTTCTGCATATAACTA‑TTTT‑‑GAAAACAA‑TT‑TA‑‑TTTTTG‑TTTTTCAAAAATTGCACGCCGGTAT‑CCGG‑AAAGTCGGTAAACACACCGGTGGCGCCGGATTTATTCAACAACGCATCATACATTTGGTTCACATCGGTGAAGAAGTCCGGTAAGGCATCTTTACGCACGGTGTATGGGTGTAATTCAATCTTGTACTGCGCTAATTCTTTGACTAAAGGCGTATATTCGATTTTGCCCGGTTTGGATTTTTCTTTGTCGATCAACATATACCAACCCGGCCCC  >  1/533822‑534302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑