Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,722,118	+A	intergenic (+2160/‑72724)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,722,117	1	.	A	100.0%	132.7 / NA	50	intergenic (+2159/‑72725)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (28/22);  total (28/22)

GTTTTGTGATCTATATCAAAAAAACTTAATTTAGTATTTTTCTTCACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTC‑ATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCACCCTGCTTCGCATTATGGCAGGCATCGATAAAG  >  1/1721869‑1722355                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               gTTTTGTGATCTATATCAAAAAAACTTAATTTAGTATTTTTCTTCACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGtc‑aa                                                                                                                                                                                                                                              >  1:445405/1‑250 (MQ=255) gTTTTGTGATCTATATCAAAAAAACTTAATTTAGTATTTTTCTTCACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGtc‑aa                                                                                                                                                                                                                                              >  1:570670/1‑250 (MQ=255)    ttGTGATCTATATCAAAAAAACTTAATTTAGTATTTTTCTTCACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGtc‑aatca                                                                                                                                                                                                                                           <  1:183453/251‑5 (MQ=255)                      aaaCTTAATTTAGTATTTTTCTTCACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGc                                                                                                                                                                                                                            >  1:753422/1‑249 (MQ=255)                                        ttcttcACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACtt                                                                                                                                                                                                        >  1:5744/1‑251 (MQ=255)                                               cacGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCtt                                                                                                                                                                                                 >  1:137685/1‑251 (MQ=255)                                               cacGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCtt                                                                                                                                                                                                 >  1:636783/1‑251 (MQ=255)                                                        aTCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCagag                                                                                                                                                                                        <  1:670669/251‑1 (MQ=255)                                                         tCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAgaga                                                                                                                                                                                       >  1:328676/1‑251 (MQ=255)                                                          cTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGtc‑aatcag                                                                                                                                                                                                                                          >  1:28461/1‑193 (MQ=255)                                                               gCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAAtt                                                                                                                                                                                 >  1:560487/1‑251 (MQ=255)                                                                cATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTATCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTa                                                                                                                                                                                <  1:452637/251‑1 (MQ=255)                                                                             cAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAAtt                                                                                                                                                                   >  1:530820/1‑251 (MQ=255)                                                                                    ttGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTtat                                                                                                                                                            >  1:98361/1‑251 (MQ=255)                                                                                              ttCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCAc                                                                                                                                                   >  1:89164/1‑250 (MQ=255)                                                                                                  tAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAAccc                                                                                                                                                                                            >  1:554642/1‑205 (MQ=255)                                                                                                  tAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGt                                                                                                                                              <  1:738986/251‑1 (MQ=255)                                                                                                      gTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGaataat                                                                                                                                                                                  <  1:168730/211‑1 (MQ=255)                                                                                                            aaataaaataATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAg                                                                                                                                               >  1:288669/1‑240 (MQ=255)                                                                                                              ataaaataaTCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAagtagt                                                                                                                                  >  1:8461/1‑251 (MQ=255)                                                                                                                                gCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCatat                                                                                                                >  1:869325/1‑251 (MQ=255)                                                                                                                                     actactTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGa                                                                                                           >  1:398420/1‑251 (MQ=255)                                                                                                                                      ctactTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGaa                                                                                                          >  1:704312/1‑251 (MQ=255)                                                                                                                                        actTTCCTACCTCTCCTCGCGCGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAAccc                                                                                                                                                                                            >  1:123169/1‑167 (MQ=255)                                                                                                                                           ttCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCag                                                                                                                                                                                          <  1:257186/166‑1 (MQ=255)                                                                                                                                           ttCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAata                                                                                                     >  1:231406/1‑251 (MQ=255)                                                                                                                                            tCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTcc                                                                                                                                                                                                    <  1:834052/155‑1 (MQ=255)                                                                                                                                            tCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATg                                                                                                                                                                              <  1:629482/177‑1 (MQ=255)                                                                                                                                            tCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAatat                                                                                                    >  1:341726/1‑251 (MQ=255)                                                                                                                                              cTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATAttt                                                                                                  >  1:225734/1‑251 (MQ=255)                                                                                                                                                    ctcctcGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCa                                                                                            >  1:134631/1‑251 (MQ=255)                                                                                                                                                           cgAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCttttt                                                                                     >  1:557693/1‑251 (MQ=255)                                                                                                                                                               agGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTccc                                                                                  <  1:524385/250‑1 (MQ=255)                                                                                                                                                                ggTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTa                                                                                                                                                                                <  1:401102/155‑1 (MQ=255)                                                                                                                                                                     ttttATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGgcgc                                                                            >  1:581079/1‑250 (MQ=255)                                                                                                                                                                          tatCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAAtt                                                                                                                                                                   <  1:3185/158‑1 (MQ=255)                                                                                                                                                                          tatCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAAt                                                                      >  1:70259/1‑251 (MQ=255)                                                                                                                                                                               gTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCg                                                                                                                                                                 >  1:424725/1‑155 (MQ=255)                                                                                                                                                                               gTTTTCCGCGAATGATTAGCACAAAGACGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCg                                                                 >  1:836638/1‑251 (MQ=255)                                                                                                                                                                                       cgAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGc                                                                                                                                          >  1:849330/1‑170 (MQ=255)                                                                                                                                                                                                        gATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCAc                                                                                                                                                                                                          >  1:858818/1‑89 (MQ=255)                                                                                                                                                                                                            aaaCATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATcc                                    <  1:560826/251‑1 (MQ=255)                                                                                                                                                                                                              aCATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCa                                   >  1:69273/1‑250 (MQ=255)                                                                                                                                                                                                                        gAATGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTa                                                                                                                                                                                <  1:739471/99‑1 (MQ=255)                                                                                                                                                                                                                          aTGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAAGTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCAc                                                                                               <  1:747605/178‑1 (MQ=255)                                                                                                                                                                                                                           tGAACCCCCGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCACCCTGCTTCGCAtt                     <  1:855053/251‑1 (MQ=255)                                                                                                                                                                                                                               cccccGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTc                                                                                                                                                                                                                   <  1:440021/57‑1 (MQ=255)                                                                                                                                                                                                                                ccccGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCACCCTGCTTCGCATTATggc                <  1:386822/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGAATGATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATCCAATTCGTTTATACGATGCAc                                                                                                                                                   <  1:348787/117‑1 (MQ=255)                                                                                                                                                                                                                                        gATTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCACCCTGCTTCGCATTATggcaggc            <  1:467299/247‑1 (MQ=255)                                                                                                                                                                                                                                         aTTAAATTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTa                                                                                                                                                                                <  1:707994/82‑1 (MQ=255)                                                                                                                                                                                                                                             aaTTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCGCTTTTCCCTTAACCCAGAGAATAATTATGTCAATAc                                                                                                                                                                       <  1:90388/87‑1 (MQ=255)                                                                                                                                                                                                                                              aTTCCATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCACCCTGCTTCGCATTATGGCAGGCATCGATAAAg  <  1:727053/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccATCGGTCAATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGaaa                                                                                                         <  1:701577/145‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               GTTTTGTGATCTATATCAAAAAAACTTAATTTAGTATTTTTCTTCACACGAAAAAATCTATAGCATTCAATCGCGTCAAGTGATTGCTTGATATTCATAAAGTTCCTAAATAAAATAATCATAAATAGCTAAACTACTTTCCTACCTCTCCTCGCGAGAGGTTTTTTTATATCTGTTTTCCGCGAATGATTAGCACAAAGATGAAACATCAAGTAGAATGAACCCCCGAATGATTAAATTCCATCGGTC‑ATCAGGTGCCGAAAAAACGCCTTGAATTCCGACCGCACTTTTCCCTTAACCCAGAGAATAATTATGTCAATACAATTCGTTTATACGATGCACCGAGTAGGCAAAGTAGTTCCGCCGAAACGTCATATTCTGAAAAATATTTCACTCAGCTTTTTCCCGGGCGCCAAAATCGGCGTACTTGGCTTAAACGGTGCCGGTAAATCCACCCTGCTTCGCATTATGGCAGGCATCGATAAAG  >  1/1721869‑1722355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑