Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,670,036	(T)6→7	intergenic (‑88/‑49847)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,670,030	1	.	T	100.0%	48.3 / NA	19	intergenic (‑82/‑49853)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (8/11);  total (8/11)

TCACAATCCGGGGCTCTACCAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTA‑TTTTTTATTCAAAGTGAATATAATTTATAAATTATCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAG  >  1/1669804‑1670193                                                                                                                                                                                                                                    |                                                                                                                                                                    tCACAATCCGGGGCTCTACCAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATaa                                                                                                                                              <  1:673811/251‑1 (MQ=255)  cacaATCCGGGGCTTTACCAACTGAGCTATTGCCGCCATTTTGAATTAGCTGAATTTGCATTGCCTTTGGCGCGCTTGACAGGAATTGAACCTGCGGCCTTTGGCTCCGGGGGCCAACGCTCTAACCAACCGAGCTACGAGCGCTTGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAAt                                                                                                                                             <  1:23159/251‑1 (MQ=255)           gggCTCTACCAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAAt                                                                                                                                     <  1:180116/250‑1 (MQ=255)           gggCTCTACCAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAAt                                                                                                                                     <  1:175855/250‑1 (MQ=255)              ctctACCAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAatat                                                                                                                                                <  1:302445/236‑1 (MQ=255)                    cAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGatta                                                                                                                           >  1:542419/1‑251 (MQ=255)                         gAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTA‑tttttttattc                                                                                                                                                          <  1:370415/214‑6 (MQ=255)                                       atatTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATaa                                                                                                                                              <  1:224253/213‑1 (MQ=255)                                       atatTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAAt                                                                                                                                     >  1:196038/1‑222 (MQ=255)                                       atatTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTaa                                                                                                         >  1:757450/1‑250 (MQ=255)                                        tatTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATGCAAAGTGAatat                                                                                                                                                <  1:669956/210‑1 (MQ=255)                                                                                    gATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTg                                                           >  1:390917/1‑251 (MQ=255)                                                                                       ttGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTgcag                                                        <  1:417979/251‑1 (MQ=255)                                                                                                                       cGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATaa                                                                                                                                              >  1:80666/1‑133 (MQ=255)                                                                                                                           ctATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTg                                                                                                                                                     <  1:561295/122‑1 (MQ=255)                                                                                                                                cAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCa                >  1:520762/1‑250 (MQ=255)                                                                                                                                       gCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCgtg        >  1:818525/1‑251 (MQ=255)                                                                                                                                               cgcTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATag  <  1:700944/249‑1 (MQ=255)                                                                                                                                               cgcTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATag  <  1:483895/249‑1 (MQ=255)                                                                                                                                                         gtcgtTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATag  >  1:463586/1‑239 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                    TCACAATCCGGGGCTCTACCAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTA‑TTTTTTATTCAAAGTGAATATAATTTATAAATTATCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAG  >  1/1669804‑1670193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑