Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,582,040	(A)7→8	intergenic (‑137412/‑59724)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,582,033	1	.	A	100.0%	96.8 / NA	37	intergenic (‑137405/‑59731)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (22/15);  total (22/15)

GGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTGCGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTT‑AAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTGAACCTGTTCCGGCGACCATTTATCCAAC  >  1/1581798‑1582274                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  ggATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGt                                                                                                                                                                                                                                     >  1:552089/1‑251 (MQ=255)                                          aCCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTT‑aaaaa                                                                                                                                                                                                                                              <  1:771693/200‑1 (MQ=255)                                                           aaTCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCcgg                                                                                                                                                                           <  1:825050/251‑1 (MQ=255)                                                                 atatTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGc                                                                                                                                                                     <  1:678244/251‑1 (MQ=255)                                                                          aaaGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAgg                                                                                                                                                            <  1:169977/251‑1 (MQ=255)                                                                             gAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGgcg                                                                                                                                                          >  1:723930/1‑250 (MQ=255)                                                                              aaaaaGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGgcgc                                                                                                                                                         <  1:29537/250‑1 (MQ=255)                                                                                        gTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCcggcgg                                                                                                                                                                        <  1:342164/225‑1 (MQ=255)                                                                                                       atttaAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                                                                                                                  >  1:702181/1‑200 (MQ=255)                                                                                                       atttaAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTa                                                                                                                               >  1:57777/1‑251 (MQ=255)                                                                                                             gggTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                                                                                                                  <  1:479488/194‑1 (MQ=255)                                                                                                             gggTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTC‑ATCAACAATTTAATATCCTTCGGCGGGGCCTTGTTAGGCCGGGAGATGATAAATAAATTTATCCCTAACTTgg                                                                                                                         >  1:699015/1‑250 (MQ=255)                                                                                                              ggTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATTGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTgg                                                                                                                         >  1:644628/1‑250 (MQ=255)                                                                                                               gTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGgcgc                                                                                                                                                         >  1:488041/1‑217 (MQ=255)                                                                                                                   aaaTTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAAt                                                                                                                   >  1:214345/1‑251 (MQ=255)                                                                                                                       tCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGc                                                                                                               <  1:278401/251‑1 (MQ=255)                                                                                                                             ttgttgAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTaa                                                                                                                                                              <  1:9838/198‑1 (MQ=255)                                                                                                                               gttgAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTAtgttgt                                                                                                       >  1:828359/1‑251 (MQ=255)                                                                                                                                    aTTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTa                                                                                                  >  1:844050/1‑251 (MQ=255)                                                                                                                                     ttGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGata                                                                                                                                               >  1:885191/1‑205 (MQ=255)                                                                                                                                       gtgGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAgg                                                                                                                                                            >  1:203827/1‑190 (MQ=255)                                                                                                                                          gAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                                                                                                                  <  1:750574/165‑1 (MQ=255)                                                                                                                                            ggggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGc                                                                                                               >  1:422546/1‑230 (MQ=255)                                                                                                                                              ggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                                                                                                                  <  1:392120/161‑1 (MQ=255)                                                                                                                                              ggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCccg                                                                                         >  1:501845/1‑250 (MQ=255)                                                                                                                                              ggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCccg                                                                                         >  1:174574/1‑250 (MQ=255)                                                                                                                                               gAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAgg                                                                                                                                                            <  1:510752/182‑1 (MQ=255)                                                                                                                                               gAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTAtgt                                                                                                          >  1:174160/1‑232 (MQ=255)                                                                                                                                                  tAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                                                                                                                  >  1:800536/1‑157 (MQ=255)                                                                                                                                                      aaGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTa                                                                                                                                                               >  1:277136/1‑172 (MQ=255)                                                                                                                                                                                  tAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAAtt                                                                                                                                       <  1:784427/168‑1 (MQ=255)                                                                                                                                                                                                        tttttAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTg                              <  1:678145/251‑1 (MQ=255)                                                                                                                                                                                                             aaGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTGAACCt                         <  1:575854/251‑1 (MQ=255)                                                                                                                                                                                                               gAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTAtgt                                                                                                          >  1:428885/1‑168 (MQ=255)                                                                                                                                                                                                                 aTTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTGAACCTGtt                      >  1:281025/1‑250 (MQ=255)                                                                                                                                                                                                                            gtgtTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGt                                                                                                                                                                >  1:498894/1‑101 (MQ=255)                                                                                                                                                                                                                            gtgtTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTGAACCTGTTCCGGCGACCAtt          >  1:230957/1‑251 (MQ=255)                                                                                                                                                                                                                                    tAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTGAACCTGTTCCGGCGACCATTTATCCAAc  <  1:709077/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  GGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTGCGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTT‑AAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCGCCGGCAACAGAAAGGAGCGTTTTTTTACTTTGCTTTTTTAACAAAGCCAATGCCTGTTGAACCTGTTCCGGCGACCATTTATCCAAC  >  1/1581798‑1582274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑