Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,544,387	(T)6→7	intergenic (‑99759/‑97377)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,544,381	1	.	T	100.0%	183.2 / NA	64	intergenic (‑99753/‑97383)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (22/42);  total (22/42)

AGGTTGTGACGCCTTTATCACCGGTGAAGTTTCGGAACAAACCATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAA‑TTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGACGTTGCCACTGATGAAAGC  >  1/1544131‑1544620                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                aGGTTGTGACGCCTTTATCACCGGTGAAGTTTCGGAACAAACCATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCaa                                                                                                                                                                                                                                                  >  1:786158/1‑251 (MQ=255)                 aTCACCGGTGAAGTTTCGGAACAAACCATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATCTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAAc                                                                                                                                                                                                                                  <  1:191924/251‑1 (MQ=255)                                 cGGAACAAACCATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAAc                                                                                                                                                                                                                                  >  1:300513/1‑235 (MQ=255)                                    aaCAAACCATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATggg                                                                                                                                                                                                               <  1:463739/251‑1 (MQ=255)                                          ccATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCtt                                                                                                                                                                                                         >  1:391169/1‑251 (MQ=255)                                             ttcattcCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAAc                                                                                                                                                                                                      <  1:376460/251‑1 (MQ=255)                                                         gagaACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAAc                                                                                                                                                                                                      >  1:267415/1‑239 (MQ=255)                                                          agaACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGt                                                                                                                                                                                            <  1:534204/248‑1 (MQ=255)                                                              cAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAg                                                                                                                                                                                     <  1:668314/251‑1 (MQ=255)                                                                  gggTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTc                                                                                                                                                                                   <  1:46190/249‑1 (MQ=255)                                                                  gggTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTc                                                                                                                                                                                   <  1:344462/249‑1 (MQ=255)                                                                     ttCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAgg                                                                                                                                                                              <  1:123710/251‑1 (MQ=255)                                                                          ttCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTa                                                                                                                                                                                                                   >  1:195617/1‑209 (MQ=255)                                                                                         catcaCGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGtt                                                                                                                                                                                                             <  1:583495/200‑1 (MQ=255)                                                                                         catcaCGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCa                                                                                                                                                          >  1:837917/1‑251 (MQ=255)                                                                                                    cGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGc                                                                                                                                               <  1:142327/251‑1 (MQ=255)                                                                                                         gTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATggg                                                                                                                                                                                                               <  1:327795/182‑1 (MQ=255)                                                                                                         gTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTa                                                                                                                                                                                                 >  1:20491/1‑196 (MQ=255)                                                                                                          ttACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGc                                                                                                                                                      <  1:569931/238‑1 (MQ=255)                                                                                                          ttACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGc                                                                                                                                               <  1:606242/245‑1 (MQ=255)                                                                                                           tACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGa                                                                                                                                        <  1:209350/251‑1 (MQ=255)                                                                                                           tACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGa                                                                                                                                        <  1:389331/251‑1 (MQ=255)                                                                                                           tACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGa                                                                                                                                        <  1:670831/251‑1 (MQ=255)                                                                                                               gggTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAg                                                                                                                                                                                                                            >  1:223368/1‑163 (MQ=255)                                                                                                               gggTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCa                                                                                                                                                                   <  1:423952/220‑1 (MQ=255)                                                                                                               gggTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGc                                                                                                                                               <  1:498990/240‑1 (MQ=255)                                                                                                               gggTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAg                                                                                                                                     >  1:465313/1‑250 (MQ=255)                                                                                                                 gTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAAc                                                                                                                                                                                                      <  1:715122/183‑1 (MQ=255)                                                                                                                 gTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGa                                                                                                                                  >  1:846194/1‑251 (MQ=255)                                                                                                                       gCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTTACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGtt                                                                                                                                                             >  1:19308/1‑218 (MQ=255)                                                                                                                        cATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGa                                                                                                                           >  1:847175/1‑251 (MQ=255)                                                                                                                          ttAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAAc                                                                                                                         <  1:38934/251‑1 (MQ=255)                                                                                                                            aGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACaa                                                                                                                       >  1:496827/1‑251 (MQ=255)                                                                                                                             ggCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACa                                                                                                                        <  1:854845/249‑1 (MQ=255)                                                                                                                               aaaggaatggcgtaatgataatGGCTTTGGGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAgg                                                                                                                     <  1:272660/230‑1 (MQ=255)                                                                                                                                      ctggcAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAAc                                                                                                             <  1:344088/251‑1 (MQ=255)                                                                                                                                              cGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCttt                                                                                                      >  1:356083/1‑250 (MQ=255)                                                                                                                                                ataatGGCTTTGGGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCAc                                                                                                   <  1:197138/248‑1 (MQ=255)                                                                                                                                                aaTATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCAc                                                                                                   <  1:576595/251‑1 (MQ=255)                                                                                                                                                aaTATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCAc                                                                                                   <  1:541996/251‑1 (MQ=255)                                                                                                                                                      gTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGt                                                                                                                                                                                            <  1:687992/156‑1 (MQ=255)                                                                                                                                                       ttttGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTAttt                                                                                             <  1:22491/250‑1 (MQ=255)                                                                                                                                                                  aaTTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAg                                                                                                                                                                                     <  1:893292/151‑1 (MQ=255)                                                                                                                                                                   aTTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGaa                                                                                                                              >  1:406060/1‑205 (MQ=255)                                                                                                                                                                   aTTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAAc                                                                                                                         >  1:816950/1‑210 (MQ=255)                                                                                                                                                                   aTTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCAc                                                                                                   <  1:297942/232‑1 (MQ=255)                                                                                                                                                                   aTTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTa                                                                                >  1:203949/1‑251 (MQ=255)                                                                                                                                                                          gATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGc                                                                                                                                               >  1:690641/1‑181 (MQ=255)                                                                                                                                                                               ggATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAAc                                                                            <  1:874677/243‑1 (MQ=255)                                                                                                                                                                                      ccGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAAt                                                             <  1:270952/251‑1 (MQ=255)                                                                                                                                                                                       cGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCAc                                                                                                   >  1:231507/1‑212 (MQ=255)                                                                                                                                                                                         gCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCAt                                                                                                                                                         <  1:366370/156‑1 (MQ=255)                                                                                                                                                                                            tAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCAc                                                                                                   <  1:663902/207‑1 (MQ=255)                                                                                                                                                                                                               tCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAAc                                                                                                             <  1:670345/178‑1 (MQ=255)                                                                                                                                                                                                               tCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACAt                                    <  1:834901/251‑1 (MQ=255)                                                                                                                                                                                                               tCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACAt                                    >  1:334150/1‑251 (MQ=255)                                                                                                                                                                                                               tCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGCTTCTTAACAGGAAAACGTATTTTAGTCACAGGtctctcc                                                                                                                                                                       >  1:35360/1‑117 (MQ=255)                                                                                                                                                                                                                        tGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAAc                                                                            <  1:722425/202‑1 (MQ=255)                                                                                                                                                                                                                         gCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCg                          >  1:896177/1‑251 (MQ=255)                                                                                                                                                                                                                                          cAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTAttt                                                                                             <  1:896800/167‑1 (MQ=255)                                                                                                                                                                                                                                          cAAGTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAACTGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGACGTTGCCAc            >  1:439965/1‑248 (MQ=255)                                                                                                                                                                                                                                             gTATAATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGAc                    <  1:727968/237‑1 (MQ=255)                                                                                                                                                                                                                                              tataATCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGACGTTGCCACtgatg       <  1:860680/249‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGACGTTGCCACTGATGAAAGc  <  1:606002/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTCTCGCCAATTTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGACGTTGCCACTGATGAAAGc  <  1:567629/251‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                AGGTTGTGACGCCTTTATCACCGGTGAAGTTTCGGAACAAACCATTCATTCCGCCAGAGAACAAGGGGTTCATTTCTTTTCCTCCGGTCATCACGCCACCGAACGTTACGGGGTGAAAGCATTAGGCGAATGGCTGGCAAACGAATATGGTTTTGAGGTGGAATTTAAGGATATGGATAATCCGGCGTAACTGGTTAAACCAAAAATCGTTACATTGCTCAAGGTCGGCAAAACAAGTATAATCTCGCCAA‑TTTTTTCACTAAAACAAATAGGAATAACTATGGGTTTCTTAACAGGTAAACGTATTTTAGTCACAGGTCTCGCCAGCAATCGTTCCATCGCCTACGGCATTGCGAAAGCGATGAAAGAACAAGGTGCGGAACTGGCTTTCACTTATTTAAATGAAAAATTACAACCGCGCGTAGAAGAATTTGCCAAAGAATTCGGTGTGAACATCGTGCTTCCGTTGGACGTTGCCACTGATGAAAGC  >  1/1544131‑1544620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑