Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	26,717	+A	intergenic (–/+104417)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	26,717	1	.	A	100.0%	145.4 / NA	54	intergenic (–/+104417)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (37/17);  total (37/17)

TCTATCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGCGCTGCTCATATTCATAAGGGCAACCAGTG‑TCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCAGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGCCCAAAAGCCCAAATTCAGCAACA  >  1/26470‑26893                                                                                                                                                                                                                                                         |                                                                                                                                                                                 tCTATCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAgtgatc                                                                                                                                                                                <  1:436167/251‑4 (MQ=255)  cTATCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAgtgatcc                                                                                                                                                                               <  1:472401/251‑5 (MQ=255)    aTCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCa                                                                                                                                                                             >  1:725647/1‑251 (MQ=255)    aTCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCa                                                                                                                                                                             >  1:125432/1‑251 (MQ=255)    aTCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCa                                                                                                                                                                             >  1:480970/1‑251 (MQ=255)       aTAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGc                                                                                                                                                                          <  1:360717/251‑1 (MQ=255)                 ggAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCggg                                                                                                                                                                >  1:848675/1‑250 (MQ=255)                 ggAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGt                                                                                                                                                                >  1:662919/1‑251 (MQ=255)                            ggCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGc                                                                                                                                                                          >  1:721439/1‑230 (MQ=255)                              cGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTaca                                                                                                                                                   >  1:201927/1‑251 (MQ=255)                                     gTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGa                                                                                                                                            >  1:50599/1‑251 (MQ=255)                                          aaaTCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCa                                                                                                                                                                             <  1:276384/213‑1 (MQ=255)                                              cATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTac                                                                                                                                                    >  1:336228/1‑234 (MQ=255)                                                tataACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCATTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAAttt                                                                                                                                 >  1:43259/1‑251 (MQ=255)                                                               gTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCa                                                                                                                  >  1:655250/1‑251 (MQ=255)                                                                 aCTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTacac                                                                                                                                                  >  1:415463/1‑217 (MQ=255)                                                                 aCTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGaa                                                                                                                                    <  1:76078/231‑1 (MQ=255)                                                                  cTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCAtt                                                                                                                >  1:842539/1‑250 (MQ=255)                                                                            tttttGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAATATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAAttttt                                                                                                     >  1:55149/1‑251 (MQ=255)                                                                                      aGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCa                                                                                                                                                                         >  1:183838/1‑173 (MQ=255)                                                                                           aaaTTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAAt                                                                                                                                   <  1:474187/206‑1 (MQ=255)                                                                                           aaaTTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCa                                                                                      >  1:705535/1‑251 (MQ=255)                                                                                            aaTTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGCAGATGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAACTTGCCGAGCCGGCAGCATTCGAGAATTCTTCTAAAGTGGACGCCAt                                                                                     >  1:585574/1‑251 (MQ=255)                                                                                                tATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTAc                                                                                                                                               >  1:378585/1‑189 (MQ=255)                                                                                                tATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAAt                                                                                                                                   <  1:8348/201‑1 (MQ=255)                                                                                                     gTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGga                                                                            >  1:708083/1‑251 (MQ=255)                                                                                                       ccGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGc                                                                                                                                                                          >  1:110603/1‑155 (MQ=255)                                                                                                       ccGTTGAAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGgaag                                                                          <  1:582164/251‑1 (MQ=255)                                                                                                          ttGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGaaa                                                                       >  1:740735/1‑251 (MQ=255)                                                                                                               gATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGAcc                                                                                                                                          >  1:190305/1‑179 (MQ=255)                                                                                                               gATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCAt                                                                                     <  1:612092/232‑1 (MQ=255)                                                                                                                 ttACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGgaag                                                                          <  1:46054/241‑1 (MQ=255)                                                                                                                      ataatGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGtttt                                                           >  1:58209/1‑251 (MQ=255)                                                                                                                              cTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTAc                                                                                                                                               >  1:534670/1‑159 (MQ=255)                                                                                                                                ttttAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCggg                                                                                                            >  1:590240/1‑192 (MQ=255)                                                                                                                                       aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTacac                                                                                                                                                  >  1:294532/1‑147 (MQ=255)                                                                                                                                       aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGgaag                                                                          <  1:458119/219‑1 (MQ=255)                                                                                                                                       aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCa                                          >  1:328278/1‑251 (MQ=255)                                                                                                                                       aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCa                                          >  1:507999/1‑251 (MQ=255)                                                                                                                                                        gTTAAAAAATAATAGCAAACCTCCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCAGTGCCGAAACGTACACTACCGACCATAGAATTTGCCg                                                                                                                             <  1:673694/151‑1 (MQ=255)                                                                                                                                                        gTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGaaa                                                                                                                                                         >  1:368503/1‑123 (MQ=255)                                                                                                                                                        gTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTAc                                                                                                                                               >  1:547594/1‑133 (MQ=255)                                                                                                                                                        gTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgcaattg                          >  1:692873/1‑250 (MQ=255)                                                                                                                                                               aataatAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  <  1:30640/251‑6 (MQ=255)                                                                                                                                                                    taGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGAcc                                                                                                                                          >  1:244868/1‑126 (MQ=255)                                                                                                                                                                    taGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggag             >  1:205039/1‑241 (MQ=255)                                                                                                                                                                    taGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggag             <  1:764158/251‑11 (MQ=255)                                                                                                                                                                    taGCAAACCACCGCCATCAACGAACACGCAGCCATGGTCAGCAACAGTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAAAGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGACAATTGCAATTGccctgtagcggag             <  1:464602/251‑11 (MQ=255)                                                                                                                                                                               cGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGACGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggagcgagcaatttg  >  1:446493/1‑230 (MQ=255)                                                                                                                                                                                      aaCGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  >  1:861/1‑223 (MQ=255)                                                                                                                                                                                                  gccATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAAt                                                                      <  1:59172/164‑1 (MQ=255)                                                                                                                                                                                                  gccATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  >  1:315677/1‑211 (MQ=255)                                                                                                                                                                                                               aCATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgc                               >  1:509933/1‑190 (MQ=255)                                                                                                                                                                                                                               gctCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgc                               <  1:110978/174‑1 (MQ=255)                                                                                                                                                                                                                                   atatTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgc                               <  1:274086/170‑1 (MQ=255)                                                                                                                                                                                                                                     atTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAAt                                                                                                                                   <  1:93618/68‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                 TCTATCATAAGGTGAAGGAACGGCGTAGGCGGGAAAGTACAAAATCATATAACTCTTTCACGGTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGCGCTGCTCATATTCATAAGGGCAACCAGTG‑TCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCAGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGCCCAAAAGCCCAAATTCAGCAACA  >  1/26470‑26893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑