Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,450,755	(A)7→8	intergenic (‑6127/‑191009)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,450,748	1	.	A	100.0%	78.0 / NA	29	intergenic (‑6120/‑191016)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (8/21);  total (8/21)

GGCTTTATTGGACAACGCACCGCCAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑AAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTAGTACGAAATTCAAAGTTTTTTGCATCCACTCCGT  >  1/1450503‑1450988                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ggCTTTATTGGACAACGCACCGCCAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑aaaaa                                                                                                                                                                                                                                             >  1:467193/1‑251 (MQ=255)   cTTTATTGGACAACGCACCGCCAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑aaaaaaa                                                                                                                                                                                                                                           >  1:627942/1‑251 (MQ=255)     ttATTGGACAACGCACCGCCAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑aaaaaaaat                                                                                                                                                                                                                                         >  1:718406/1‑249 (MQ=255)                    ccGCCAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGc                                                                                                                                                                                                                            >  1:162883/1‑250 (MQ=255)                       ccAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGAc                                                                                                                                                                                                                        <  1:439374/251‑1 (MQ=255)                                     cAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCt                                                                                                                                                                                                             <  1:107335/248‑1 (MQ=255)                                                    cAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATAc                                                                                                                                                                                           <  1:242558/251‑1 (MQ=255)                                                      aaaTGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACgg                                                                                                                                                                                         <  1:889815/251‑1 (MQ=255)                                                           gCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAg                                                                                                                                                                                     <  1:651296/250‑1 (MQ=255)                                                                 ggCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTaa                                                                                                                                                                                      >  1:487249/1‑243 (MQ=255)                                                                     aCATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCGTTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATAc                                                                                                                                                                                           >  1:435981/1‑234 (MQ=255)                                                                                tttCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTatat                                                                                                                                                                                                <  1:79465/218‑1 (MQ=255)                                                                                              aaGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTAc                                                                                                                                                 <  1:262006/251‑1 (MQ=255)                                                                                                 ttgttTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACgg                                                                                                                                               <  1:11647/250‑1 (MQ=255)                                                                                                          cATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACAcc                                                                                                                                     <  1:617068/251‑1 (MQ=255)                                                                                                               aTTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGt                                                                                                                                                                                                    <  1:702499/183‑1 (MQ=255)                                                                                                                      cctcTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATg                                                                                                                                                                                                     <  1:372909/175‑1 (MQ=255)                                                                                                                      cctcTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGt                                                                                                                                                                                                    <  1:326809/176‑1 (MQ=255)                                                                                                                      cctcTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTc                                                                                                                                                      <  1:575495/222‑1 (MQ=255)                                                                                                                      cctcTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTgcag                                                                                                                          >  1:508684/1‑250 (MQ=255)                                                                                                                                                          atTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTAtattat                                                                                                                                                                                             >  1:90163/1‑147 (MQ=255)                                                                                                                                                          atTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGAttt                                                                                      >  1:675857/1‑250 (MQ=255)                                                                                                                                                                            tCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACgg                                                                                                                                                                                         <  1:235193/133‑1 (MQ=255)                                                                                                                                                                                  tatTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGta                                                                                                                                                                                                   <  1:91578/117‑1 (MQ=255)                                                                                                                                                                                  tatTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATAc                                                             <  1:588768/251‑1 (MQ=255)                                                                                                                                                                                            tttttGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTATATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGc                                                   <  1:310405/251‑1 (MQ=255)                                                                                                                                                                                                    atatTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAAt                                                                                                                                                                                                                     <  1:129725/81‑1 (MQ=255)                                                                                                                                                                                                          cTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATc                                                                                                                                                                                                              >  1:479778/1‑82 (MQ=255)                                                                                                                                                                                                          cTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGt                                                                                                                                                                                                    <  1:812543/92‑1 (MQ=255)                                                                                                                                                                                                          cTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCAt                                            <  1:406627/244‑1 (MQ=255)                                                                                                                                                                                                                 aCTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGa                              <  1:384318/251‑1 (MQ=255)                                                                                                                                                                                                                                             cATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGt  >  1:589763/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GGCTTTATTGGACAACGCACCGCCAATCCCTAACAGCAAACCGGCAGCGGGCAAAATGGCAATAGGCAACATAAATGCCTTTCCGACTTGTTGAAGTTGTTTAAACATAAATTCTCTCCTCTTCACGACGCAATTTATGATTTATCTTGAAATATTTTATGGAGTTACATTTCTAAATATTAACTATTTTTTGGAATATTACTTAAATACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑AAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTAGTACGAAATTCAAAGTTTTTTGCATCCACTCCGT  >  1/1450503‑1450988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑