Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,269,531	+T	intergenic (+109951/+151406)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,269,527	1	.	T	100.0%	141.9 / NA	54	intergenic (+109947/+151410)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (29/25);  total (29/25)

GCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG‑CGCGCAACAAATAAAAATGCCCGAATAACATCGAGCATTATTTACCGGGCTGCAAGCCTTCGCCCTGCA‑TTTTCCGCAAGC‑GGAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTTATAAGTGT  >  1/1269302‑1269746                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             gCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagcttgctcaaaaatagtt                                                                                                                                                                                                     >  1:236940/1‑230 (MQ=255)  ccTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagcttgcccaaaaatagtt                                                                                                                                                                                                     <  1:346280/250‑22 (MQ=255)                taaataTTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACtt                                                                                                                                                                                       <  1:844796/251‑1 (MQ=255)                         aTTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTaaaa                                                                                                                                                                              <  1:784907/251‑1 (MQ=255)                          tttATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                                                                                                                             >  1:268366/1‑251 (MQ=255)                            tATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGTAAATTTTTATTTCTTGATT‑AAATTTTGGGACGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTATCGGGCTGCAACCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCa                                                                                                                                                                           >  1:711642/1‑251 (MQ=255)                                  gACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                                                                                                                             >  1:426022/1‑243 (MQ=255)                                           aaaaatgtAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAg                                                                                                                                                            <  1:756241/246‑1 (MQ=255)                                            aaaatgtAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGt                                                                                                                                                           <  1:739068/247‑1 (MQ=255)                                                        tAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTc                                                                                                                                                                <  1:791023/234‑1 (MQ=255)                                                            ttCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGAtttt                                                                                                                                           >  1:341661/1‑251 (MQ=255)                                                                    cGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                                                                                                                          >  1:613501/1‑228 (MQ=255)                                                                     gTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                                                                                                                          <  1:303813/227‑1 (MQ=255)                                                                     gTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCAATTCTCGATTTAATTTATGGA‑CGCGGAAAAAATAAAAATGCCCGAATAACATTCTGCCTGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGGAAAATCACTATATCTg                                                                                                                                                                  >  1:226758/1‑219 (MQ=255)                                                                       aTCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCAatttcgttaaaaaactatcaaac                                                                                                                                <  1:297496/251‑20 (MQ=255)                                                                          gCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                                                                                                                          >  1:531319/1‑222 (MQ=255)                                                                               aTCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTatat                                                                                                                                                                     >  1:171824/1‑206 (MQ=255)                                                                               aTCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAg                                                                                                                        >  1:790901/1‑251 (MQ=255)                                                                                        gcagTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATg                                                                                                               >  1:498703/1‑251 (MQ=255)                                                                                           gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                                                                                                                             <  1:799159/186‑1 (MQ=255)                                                                                           gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCa                                                                                                                                                       <  1:400230/208‑1 (MQ=255)                                                                                           gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGaaa                                                                                                            >  1:41444/1‑251 (MQ=255)                                                                                            ttattaCGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGaaa                                                                                                            <  1:521567/250‑1 (MQ=255)                                                                                               ttaCGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAAcc                                                                                                        <  1:875811/251‑1 (MQ=255)                                                                                                      gcTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTTAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCa                                                                                                 >  1:484113/2‑251 (MQ=255)                                                                                                      gcTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACCAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCa                                                                                                 >  1:575145/2‑251 (MQ=255)                                                                                                       cTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttg                                                                                                                                                                                                                        >  1:233728/1‑128 (MQ=255)                                                                                                         ttAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTc                                                                                                                                                                >  1:723725/1‑185 (MQ=255)                                                                                                                tATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagc                                                                                                                                                                                                                     >  1:720040/1‑119 (MQ=255)                                                                                                                   ttGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGttattt                                                                                    >  1:689263/1‑251 (MQ=255)                                                                                                                         gggCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTtattttattt                                                                               <  1:712035/250‑1 (MQ=255)                                                                                                                                  aTTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGac                                                                     <  1:850041/251‑1 (MQ=255)                                                                                                                                           ttctcgattaaatttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGa                                                             <  1:203027/231‑1 (MQ=255)                                                                                                                                             ctcgattaaatttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAAc                                                           <  1:322230/233‑1 (MQ=255)                                                                                                                                             ctcgattaaatttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAAc                                                           <  1:717953/233‑1 (MQ=255)                                                                                                                                                 attaaatttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTc                                                                                                                                                                <  1:89368/132‑1 (MQ=255)                                                                                                                                                  ttaaatttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATca                                                      <  1:137407/238‑1 (MQ=255)                                                                                                                                                     aatttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACAt                                                   <  1:212832/241‑1 (MQ=255)                                                                                                                                                      atttaggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAAc                                                           >  1:275550/10‑242 (MQ=255)                                                                                                                                                           ggtacgcgcAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATca                                                      >  1:57031/5‑242 (MQ=255)                                                                                                                                                                     aaaaaTAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATc                                                                                                                                                                            >  1:524522/3‑114 (MQ=255)                                                                                                                                                                       aaaTAAAAATGCCCGAATAACATCGAGCATGGCTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTa                                 >  1:721810/1‑251 (MQ=255)                                                                                                                                                                          tAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACAt                              <  1:18069/251‑1 (MQ=255)                                                                                                                                                                            aaaaTGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATc                             <  1:791560/250‑1 (MQ=255)                                                                                                                                                                                tGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGa                        <  1:321878/251‑1 (MQ=255)                                                                                                                                                                                 gCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGa                        >  1:251811/1‑250 (MQ=255)                                                                                                                                                                                  cccGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGAc                       <  1:315815/250‑1 (MQ=255)                                                                                                                                                                                        tAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTcg                >  1:624089/1‑251 (MQ=255)                                                                                                                                                                                          aCATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGa                        >  1:92661/1‑241 (MQ=255)                                                                                                                                                                                          aCATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTcgcg              >  1:796521/1‑251 (MQ=255)                                                                                                                                                                                           cATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGt             >  1:871823/1‑251 (MQ=255)                                                                                                                                                                                               gagcatggtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTta         >  1:21991/9‑251 (MQ=255)                                                                                                                                                                                                    tggtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTTATAAgt    >  1:283417/4‑251 (MQ=255)                                                                                                                                                                                                     ggtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTTATAAgtg   >  1:119271/3‑251 (MQ=255)                                                                                                                                                                                                      gtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAGACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTTATAAgtgt  >  1:639394/2‑251 (MQ=255)                                                                                                                                                                                                      gtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTtattttattt                                                                               <  1:587545/173‑1 (MQ=255)                                                                                                                                                                                                      gtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGttt           <  1:882242/241‑1 (MQ=255)                                                                                                                                                                                                      gtttACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTTATAAgtgt  >  1:828510/2‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             GCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG‑CGCGCAACAAATAAAAATGCCCGAATAACATCGAGCATTATTTACCGGGCTGCAAGCCTTCGCCCTGCA‑TTTTCCGCAAGC‑GGAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGACCACCAAGAACAATCACATCGCCATTTTTTACACTTACATCTGTGACAATGTCGCGTTTTATAAGTGT  >  1/1269302‑1269746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑