Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	862,963	+G	intergenic (‑13306/‑59255)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	862,962	1	.	G	100.0%	104.2 / NA	39	intergenic (‑13305/‑59256)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/13);  total (26/13)

AAAAATTCGGAATGCGTAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACACGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATC  >  1/862703‑863188                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   aaaagtcggaaagtgaaaaaCTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCAT‑‑‑ATATGT‑‑TTATACGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                                                                                                                                                                                                                                     <  1:223172/234‑1 (MQ=255)             gtgtaaaaCTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcc                                                                                                                                                                                                                               <  1:396556/239‑5 (MQ=255)                         ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                                                                            <  1:710923/251‑1 (MQ=255)                             gaaaaacggggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGC‑‑‑‑‑CATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                                                                            <  1:143318/239‑1 (MQ=255)                                                                                     gccatatATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                                                                            >  1:593899/3‑201 (MQ=255)                                                                                        atatatGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGatg                                                                                                                                                                                                          <  1:762781/200‑3 (MQ=255)                                                                                         tatatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCa                                                                                                                                                      >  1:135765/1‑251 (MQ=255)                                                                                          atatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                                                                            >  1:819786/1‑196 (MQ=255)                                                                                          atatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg                                                                                                                                                     >  1:693307/1‑251 (MQ=255)                                                                                          atatgGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAg                                                                                                                                                     >  1:231242/1‑251 (MQ=255)                                                                                               gTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATc                                                                                                                                                 >  1:357354/1‑250 (MQ=255)                                                                                                    acttaattcaggtttttAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTAAAGAAATATCATGTTAAAAAAATACTTACTTATTTTACCGTTTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                                                                                                                                                                                                            <  1:840948/173‑1 (MQ=255)                                                                                                    acGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGAt                                                                                                                                           >  1:215132/1‑251 (MQ=255)                                                                                                                                 gcgggacAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGaaaaa                                                                                                              >  1:772262/7‑251 (MQ=255)                                                                                                                                    ggacAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAAtt                                                                                                            >  1:23965/4‑250 (MQ=255)                                                                                                                                                    aTTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCAt                                                                                                                                                                                                                 >  1:409835/1‑133 (MQ=255)                                                                                                                                                                  ggAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttgt                                                                             >  1:417865/1‑251 (MQ=255)                                                                                                                                                                                       acttacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTc                                                                                                                                                                                      >  1:673166/1‑125 (MQ=255)                                                                                                                                                                                        cttacttaTTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaag                                                        >  1:586646/1‑250 (MQ=255)                                                                                                                                                                                          tacttATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagcaa                                                     >  1:664378/1‑251 (MQ=255)                                                                                                                                                                                            cttattttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATAttg                                                                              >  1:318695/5‑224 (MQ=255)                                                                                                                                                                                               attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                >  1:126569/2‑251 (MQ=255)                                                                                                                                                                                               attttACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGa                                                >  1:267454/2‑251 (MQ=255)                                                                                                                                                                                                           tttttttaaaCCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAGCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                    <  1:783462/243‑1 (MQ=255)                                                                                                                                                                                                            cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAatat                                                                                >  1:161632/1‑206 (MQ=255)                                                                                                                                                                                                            cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgcaagca                                                      >  1:162855/1‑232 (MQ=255)                                                                                                                                                                                                            cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                           >  1:157371/1‑227 (MQ=255)                                                                                                                                                                                                            cTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTAAGCAAAAAAAAACTTGGAATTTTGTTGGTTGCGGGAATTGGGAAAGAAATGGGACGAACGTGTTatt                                   >  1:157874/1‑249 (MQ=255)                                                                                                                                                                                                              aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                  >  1:335878/1‑248 (MQ=255)                                                                                                                                                                                                              aTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                  >  1:421049/1‑248 (MQ=255)                                                                                                                                                                                                                      cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGTTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTActg                                  <  1:804429/242‑3 (MQ=255)                                                                                                                                                                                                                      cGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGaaa                         >  1:107284/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTATTGCCCTTGAAAAACGCTTATTaa             >  1:412543/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttattaTCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACgcttatta              <  1:521803/250‑8 (MQ=255)                                                                                                                                                                                                                                   tattatCCTTATCCGCAATTTGACCCTGGTTACCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCCCCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaa            <  1:585978/251‑1 (MQ=255)                                                                                                                                                                                                                                   tattatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTAc                                    <  1:248727/227‑1 (MQ=255)                                                                                                                                                                                                                                    attatCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTaaaa           >  1:136211/1‑251 (MQ=255)                                                                                                                                                                                                                                       atccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGat        <  1:474733/251‑1 (MQ=255)                                                                                                                                                                                                                                        tccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGgc                                                           <  1:312469/199‑1 (MQ=255)                                                                                                                                                                                                                                        tccttatccGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGAATAATGATGATGAAGAAAATGCCGTTTTCAGCGCCCGCCGCACGCATGAACCGAAATATc                                                                                                                                                       <  1:185424/107‑1 (MQ=255)                                                                                                                                                                                                                                             atccGCAATTTGACCCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGTTTTCAGCACCCGCCGCACGCATGAACCGAAATATCAGGATCCGAGATACAAAATTTCCACAGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACTTGGAATATTGTTTGTTGCCGGAATTGGGCAAGCAATCGGACGAACGTGTTACTGCCCTTGAAAAACGCTTATTAAAAGATATGATc  <  1:802080/251‑1 (MQ=255)                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   AAAAATTCGGAATGCGTAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCATATCATATATAGTTACACGTAATTCGGACTTTTAAGGTTGCTGCAGCGAGGCAATGATTCATCTATTTTCAACCT‑AAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAGCCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTTCAGCATCCGCCGCACGCATGAACCGAAATATCAGGATCCAAGATACAAAATTTCCACGGAAGACATGGAAAAATTCATTTTAGCCAAAAATAACCTGGAATATTGTTTGTTACCGGAATTGGGCAAGCAATCGGATAAACGTGTTACCGCCCTTGAAAAACGGCTATTAAAAGATATGATC  >  1/862703‑863188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑