Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,899	(A)5→6	intergenic (‑12242/‑60319)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,894	1	.	A	100.0%	35.2 / NA	15	intergenic (‑12237/‑60324)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (8/7);  total (8/7)

GTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACAT  >  1/861651‑862108                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                       gTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCT‑aaaaaa                                                                                                                                                                                                                  >  1:895687/1‑250 (MQ=255)          cgtatTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttta                                                                                                                                                                                                          >  1:512730/2‑250 (MQ=255)                                                                 tGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg                                                                                                                                                  <  1:654655/251‑1 (MQ=255)                                                                       aTTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGc                                                                                                                                             >  1:276276/1‑250 (MQ=255)                                                                                                                               caaaaaCACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTg                                                                                                                                                                                      <  1:438452/153‑1 (MQ=255)                                                                                                                                     caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttt                                                                                                                                                                                                           >  1:104600/2‑128 (MQ=255)                                                                                                                                     caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg                                                                                                                                                  >  1:66297/2‑184 (MQ=255)                                                                                                                                     caCTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACtt                                                                                      <  1:578954/243‑1 (MQ=255)                                                                                                                                       cTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTg                                                                             >  1:563103/1‑251 (MQ=255)                                                                                                                                               tgtgtgGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaacaagca                                                                                                    <  1:813579/220‑4 (MQ=255)                                                                                                                                                       tttAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGaa                                                                                                          <  1:702739/206‑1 (MQ=255)                                                                                                                                                                                                                cTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTatat                                                                                                                                                    >  1:761698/1‑107 (MQ=255)                                                                                                                                                                                                                cTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACa   >  1:48135/1‑251 (MQ=255)                                                                                                                                                                                                                 ttttttAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGAACCGCTAGATAACAAGCGTTTACGTTCTTTATGCGGGGCGGGGGATAAACACC‑TTTCTTTAATTAAAAAACAt  <  1:552195/251‑1 (MQ=255)                                                                                                                                                                                                                  tttttAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCCACTTTTTTGCTCACTTTTGAGGCAAGGTTTTGAACAAGCAATTTAGCCAAACTTTCACTCTTGaacc                                                                         <  1:207156/180‑3 (MQ=255)                                                                                                                                                                                                                                aaaTCAAATTTTGTTTTTTTTCTAAAAAAGTATA‑TTTATGGAAGAGCAATTTTATTGCATCTAGTCTCTTTCGATGCAAATTGTGCTATATTg                                                                                                                                                  >  1:155335/1‑93 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                       GTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTTATGGAAGAGCAATTTTATTGCGTCTAGTCTCTTTCGATGCAAATTGTGCTATATTGTCTGCTACTTTTTTGTTCACTTTTGAGGCAGGGTTTTGAACAAACAATTTAACCGAACTTTCACTCTTGAATCGCAAGATAGCAAGCGTTTACGTTTTTTATGCGGAGCGGGGGATAAACACCTTTTCTTTAATTAAAAAACAT  >  1/861651‑862108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑