Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	861,678	+C	intergenic (‑12021/‑60540)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	861,678	1	.	C	100.0%	42.0 / NA	17	intergenic (‑12021/‑60540)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (8/9);  total (8/9)

TGCTTTCGTAATTAAGGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAATGTCATTTATTTAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTT  >  1/861463‑861908                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         tGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTa                                                                                                                                                                                                         <  1:484344/251‑1 (MQ=255)                             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGtt                                                                                                                                                                                                                      <  1:199993/210‑1 (MQ=255)                             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGa                                                                                                                                                                             >  1:225005/1‑251 (MQ=255)                               cTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAggg                                                                                                                                                                                                                 >  1:497732/1‑213 (MQ=255)                                                                                                       taataaGCTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATcgcg                                                                                                  >  1:354355/1‑251 (MQ=255)                                                                                                              cTTGTGGAATTAAATAAACGACCGGTATATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTg                                                                                                                                                                                                           >  1:151415/1‑140 (MQ=255)                                                                                                                                  accggtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGaa                                                                        <  1:232544/244‑1 (MQ=255)                                                                                                                                      gtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACt                                                                                                                                                                                        <  1:268495/133‑1 (MQ=255)                                                                                                                                      gtatatAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTC‑ttttttct                                                                                >  1:56866/3‑234 (MQ=255)                                                                                                                                                                                  taaATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTAc                                                                                                                                                            <  1:146277/119‑1 (MQ=255)                                                                                                                                                                                            atgTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTctct                                                                                                                                                                                  <  1:503919/85‑4 (MQ=255)                                                                                                                                                                                            atgTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACt                                                                                                                                                 <  1:180167/118‑1 (MQ=255)                                                                                                                                                                                            atgTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACaaaaacacttaaa                                                                                                                            >  1:385564/3‑133 (MQ=255)                                                                                                                                                                                              gTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGt                                                                                                                                                                                                          <  1:318244/61‑1 (MQ=255)                                                                                                                                                                                              gTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCT‑aaaaaa          >  1:895687/1‑250 (MQ=255)                                                                                                                                                                                                     tacgtatTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTAc                                                                                                                                                                                         <  1:150601/68‑1 (MQ=255)                                                                                                                                                                                                       cgtatTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTAC‑AAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAAAGTCGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTTTTTTTCTAAAAAAGTATAttta  >  1:512730/2‑250 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         TGCTTTCGTAATTAAGGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAATGTCATTTATTTAATAATCTTGTGGAATTAAATAAACGAGTGGCATATAGTCCTGAAAAATTAAAATGCAAG‑TTTTTTAGAATAATAAATTCACTACGTATATGTATGTATTGTGATTGCGAAAA‑TTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCCCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAAATACTTAAATATGTGTGGATTTAGAGATGTCGCGATTTATTTC‑TTTTTCCTTGTAGGAAAGTTGTATTTTTTACGCTTTTTTAACTTAACTAAATCAAATTTTGTTGTTTTTCT‑AAAAAGTATATTTT  >  1/861463‑861908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑