Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,886,586	(A)6→5	intergenic (+91659/‑47745)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,886,581	0	A	.	100.0%	291.9 / NA	69	intergenic (+91654/‑47750)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/42);  total (27/42)

CCGTCCGCCTGTGCCGGCACTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCACAAAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGTTCAGCGGTTCTTCCTGACACCACACAAAATC  >  1/1886334‑1886817                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             ccGTCCGCCTGTGCCGGCACTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCAACACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCACaaaa                                                                                                                                                                                                                                           >  1:579464/1‑251 (MQ=255)           gtgCCGGCACTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGaa                                                                                                                                                                                                                                >  1:438553/1‑251 (MQ=255)           gtgCCGGCACTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGaa                                                                                                                                                                                                                                >  1:208208/1‑251 (MQ=255)                 gCACTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATc                                                                                                                                                                                                                           <  1:759373/250‑1 (MQ=255)                    cTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCAGCTTTTTTGTGCCAAGTTGCCACGGCGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATACTGTTTATTCCTTTTTATGCTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCatcg                                                                                                                                                                                                                       >  1:318995/1‑248 (MQ=255)                        aaGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCa                                                                                                                                                                                                                     >  1:578085/1‑249 (MQ=255)                             ccacTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTAt                                                                                                                                                                                                               >  1:288965/1‑250 (MQ=255)                             ccacTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATc                                                                                                                                                                                                              <  1:888817/251‑1 (MQ=255)                               acTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCg                                                                                                                                                                                                             >  1:644314/1‑250 (MQ=255)                                cTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGcc                                                                                                                                                                                                           <  1:533337/251‑1 (MQ=255)                                 tttGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCt                                                                                                                                                                                                          <  1:130908/251‑1 (MQ=255)                                         tctcGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACg                                                                                                                                                                                                  <  1:549149/251‑1 (MQ=255)                                          ctcGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACgt                                                                                                                                                                                                 <  1:424192/251‑1 (MQ=255)                                           tcGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACgtg                                                                                                                                                                                                <  1:435497/251‑1 (MQ=255)                                            cGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGcc                                                                                                                                                                                                           >  1:796503/1‑239 (MQ=255)                                                 tCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAAcg                                                                                                                                                                                          <  1:548052/251‑1 (MQ=255)                                                           cTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATc                                                                                                                                                                                                              >  1:58771/1‑221 (MQ=255)                                                           cTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTcca                                                                                                                                                                                 >  1:774694/1‑250 (MQ=255)                                                            ttCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTccacc                                                                                                                                                                               <  1:685867/251‑1 (MQ=255)                                                                  aCGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTAt                                                                                                                                                                                                               <  1:423177/213‑1 (MQ=255)                                                                  aCGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGt                                                                                                                                                                         >  1:519251/1‑251 (MQ=255)                                                                       tCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACg                                                                                                                                                                                                  <  1:94793/221‑1 (MQ=255)                                                                       tCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTccac                                                                                                                                                                                <  1:225303/239‑1 (MQ=255)                                                                       tCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTttg                                                                                                                                                                    >  1:201517/1‑251 (MQ=255)                                                                            gCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCAc                                                                                                                                                                                                                    <  1:239794/198‑1 (MQ=255)                                                                             cGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGttt                                                                                                                                                              <  1:105717/251‑1 (MQ=255)                                                                                  cccGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGgtg                                                                                                                                                          <  1:134758/250‑1 (MQ=255)                                                                                        tttttGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGc                                                                                                                                                                                                            >  1:377958/1‑194 (MQ=255)                                                                                         ttttGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGa                                                                                                                                                  <  1:604656/251‑1 (MQ=255)                                                                                            tgtgCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGatat                                                                                                                                               <  1:164823/251‑1 (MQ=255)                                                                                              tgCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGatatat                                                                                                                                             <  1:147609/251‑1 (MQ=255)                                                                                              tgCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATATTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGatatat                                                                                                                                             <  1:333753/251‑1 (MQ=255)                                                                                                ccAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAg                                                                                                                                           <  1:348079/251‑1 (MQ=255)                                                                                                ccAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAg                                                                                                                                           <  1:814023/251‑1 (MQ=255)                                                                                                 cAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGc                                                                                                                                          <  1:50309/251‑1 (MQ=255)                                                                                                           cGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTgg                                                                                                                                >  1:72451/1‑251 (MQ=255)                                                                                                              tGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGgag                                                                                                                              <  1:878674/250‑1 (MQ=255)                                                                                                                  aTCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGagaggca                                                                                                                         >  1:876839/1‑251 (MQ=255)                                                                                                                            gATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGcc                                                                                                                                                                                                           <  1:410396/159‑1 (MQ=255)                                                                                                                             aTTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCg                                                                                                              <  1:497176/251‑1 (MQ=255)                                                                                                                                               cAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAAttt                                                                                            <  1:19079/251‑1 (MQ=255)                                                                                                                                                  gAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCg                                                                                          >  1:333786/1‑250 (MQ=255)                                                                                                                                                             gTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTcc                                                                                                                                                                                  >  1:375990/1‑151 (MQ=255)                                                                                                                                                              tCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGatatat                                                                                                                                             <  1:211396/187‑1 (MQ=255)                                                                                                                                                              tCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGgag                                                                                                                              <  1:39338/202‑1 (MQ=255)                                                                                                                                                              tCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAAt                                                                             <  1:531499/251‑1 (MQ=255)                                                                                                                                                              tCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAAt                                                                             >  1:391309/1‑251 (MQ=255)                                                                                                                                                                    tGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATc                                                                                                                                                                                                              <  1:452226/116‑1 (MQ=255)                                                                                                                                                                    tGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGatatat                                                                                                                                             <  1:470706/181‑1 (MQ=255)                                                                                                                                                                    tGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCg                                                                        >  1:240576/1‑250 (MQ=255)                                                                                                                                                                     gTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAAttt                                                                                            >  1:882006/1‑229 (MQ=255)                                                                                                                                                                     gTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAAt                                                                             >  1:880979/1‑244 (MQ=255)                                                                                                                                                                            ccTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGc                                                                                                                                          <  1:733834/176‑1 (MQ=255)                                                                                                                                                                             cTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAAtt                                                              >  1:129762/1‑251 (MQ=255)                                                                                                                                                                                        cGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCAt                                                                                                      <  1:271187/200‑1 (MQ=255)                                                                                                                                                                                        cGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTgg                                                   >  1:150456/1‑251 (MQ=255)                                                                                                                                                                                                 aaGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCAcg                                          <  1:537398/251‑1 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAAcg                                                                                                                                                                                          <  1:293696/105‑1 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGgag                                                                                                                              >  1:488213/1‑165 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAAc                                                                          >  1:18607/1‑217 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCAcgcg                                        >  1:95029/1‑251 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCAcgcg                                        >  1:536767/1‑251 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCG‑CCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCAcgcg                                        >  1:116536/1‑250 (MQ=255)                                                                                                                                                                                                   gTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCG‑TTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATAc                                              <  1:724255/244‑1 (MQ=255)                                                                                                                                                                                                      ttATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCt                                     <  1:299560/251‑1 (MQ=255)                                                                                                                                                                                                          atTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGt                                 <  1:178367/251‑1 (MQ=255)                                                                                                                                                                                                            tttGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGTTc                               <  1:409716/251‑1 (MQ=255)                                                                                                                                                                                                                              cggtTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGTTCAGCGGTTCTTCCTGacac             <  1:878637/251‑1 (MQ=255)                                                                                                                                                                                                                              cggtTTTCGCCGCGTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTACGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGTTCAGCGGTTCTTCCTGacac             <  1:328183/251‑1 (MQ=255)                                                                                                                                                                                                                                          cgTTTTTTTCGCAC‑AAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGTTCAGCGGTTCTTCCTGACACCACACAAAATc  <  1:20249/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             CCGTCCGCCTGTGCCGGCACTTCAAGCACCACTTTGTCGGTCTCGATTTCCACAATCACTTCATCACGTTTCACTGCGTCGCCCGCTTTTTTGTGCCAAGTTGCCACGGTGGCATCGGCTACGGATTCGGGTAAATCGGGGACAAGAATTTCAATCGTCATAATGTTTATTCCTTTTTATGTTCGTTTAGCTAAGTCTTATATTTTGATGAAAAGTGCGGTCGGTTTTCGCCGCGTTTTTTTCGCACAAAAAACGCTGTGAAAAATCACCGCACTTTATCGCCTCATTAACGTGTTAACGCGTCTTCCACCAATTGTTTTTGTTGTTTGGTGTGCAATGATATATAGCCCACCGCTGGAGAGGCAGAGGCCGGGCGACCGGCATAGGTTAATTTCGCCCCTTCCGGAATAACCGCTTCAAAATTGTGTTTGCTGGAATACCACGCGCCTTGGTTCAGCGGTTCTTCCTGACACCACACAAAATC  >  1/1886334‑1886817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑