Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,775,541	(T)8→7	intergenic (+55583/‑19301)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,775,534	0	T	.	100.0%	281.6 / NA	67	intergenic (+55576/‑19308)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (34/33);  total (34/33)

CGCCTTAGCGATTGCAAAGTTAGAAAATCTGGAATTAACGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGT  >  1/1775284‑1775776                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   cGCCTTAGCGATTGCAAAGTTAGAAAATCTGGAATTAACGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAAt                                                                                                                                                                                                                                                    <  1:853425/251‑1 (MQ=255)   ccTTAGCGATTGCAAAGTTAGAAAATCTGGAATTAACGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAAttt                                                                                                                                                                                                                                                  <  1:338190/251‑1 (MQ=255)                   gTTAGAAAATCTGGAATTAACGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTg                                                                                                                                                                                                                                 >  1:403221/1‑251 (MQ=255)                               ggAATTAACGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTtagaaata                                                                                                                                                                                                                     >  1:169169/1‑251 (MQ=255)                                       cGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAAt                                                                                                                                                                                                                                                    <  1:43232/213‑1 (MQ=255)                                       cGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAat                                                                                                                                                                                                             >  1:750474/1‑251 (MQ=255)                                           tGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATaa                                                                                                                                                                                                         <  1:732645/251‑1 (MQ=255)                                             cccATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAAt                                                                                                                                                                                                        <  1:408336/250‑1 (MQ=255)                                             cccATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATg                                                                                                                                                                                                       <  1:397093/251‑1 (MQ=255)                                             cccATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGACAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATg                                                                                                                                                                                                       <  1:88184/251‑1 (MQ=255)                                               cATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTc                                                                                                                                                                                                     <  1:364958/251‑1 (MQ=255)                                                   gggAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCa                                                                                                                                                                                                                               >  1:285662/1‑221 (MQ=255)                                                        aTTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTTAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAatat                                                                                                                                                                                                           <  1:719043/236‑1 (MQ=255)                                                            tCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATCA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATg                                                                                                                                                                                                       >  1:705205/1‑236 (MQ=255)                                                              cATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTAtgtt                                                                                                                                                                                      <  1:123781/251‑1 (MQ=255)                                                                      gCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAAt                                                                                                                                                                                                        >  1:155553/1‑225 (MQ=255)                                                                       cGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTAtttt                                                                                                                                                                              <  1:365180/250‑1 (MQ=255)                                                                           aTTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAAt                                                                                                                                                                                                        <  1:780831/220‑1 (MQ=255)                                                                                        atatAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAgg                                                                                                                                                            >  1:341817/1‑251 (MQ=255)                                                                                        atatAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAgg                                                                                                                                                            >  1:35082/1‑251 (MQ=255)                                                                                        atatAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAgg                                                                                                                                                            >  1:836020/1‑251 (MQ=255)                                                                                         tataAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGCTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGga                                                                                                                                                           >  1:568641/1‑251 (MQ=255)                                                                                             aaaCCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGc                                                                                                                                                                 <  1:302730/241‑1 (MQ=255)                                                                                                ccTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGaaaaaa                                                                                                                                                    <  1:537832/251‑1 (MQ=255)                                                                                                   ctcCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTtagaaatagaa                                                                                                                                                                                                                  >  1:7463/1‑186 (MQ=255)                                                                                                   ctcCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGc                                                                                                                                                                 >  1:651478/1‑235 (MQ=255)                                                                                                   ctcCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAAGCAATAGCCGTTATTTGCATCGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATg                                                                                                                                                                                                       <  1:55102/197‑1 (MQ=255)                                                                                                      cTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCAt                                                                                                                                                                                                                              >  1:143353/1‑171 (MQ=255)                                                                                                        gCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGaaaaaa                                                                                                                                                    >  1:381526/1‑243 (MQ=255)                                                                                                          cATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTtta                                                                                                                                          <  1:850156/251‑1 (MQ=255)                                                                                                           aTTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAAt                                                                                                                                                                                                        >  1:765918/1‑188 (MQ=255)                                                                                                           aTTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTttat                                                                                                                                         >  1:419654/1‑251 (MQ=255)                                                                                                           aTTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTttat                                                                                                                                         >  1:64543/1‑251 (MQ=255)                                                                                                             tCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTttatta                                                                                                                                       >  1:172854/1‑251 (MQ=255)                                                                                                                 aTGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCca                                                                                                                                   >  1:747392/1‑251 (MQ=255)                                                                                                                     tGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCgcgc                                                                                                                                                                                                >  1:557563/1‑186 (MQ=255)                                                                                                                      ggTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCAt                                                                                                                                                                                                                              >  1:261086/1‑155 (MQ=255)                                                                                                                              ccACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGt                                                                                                                      >  1:266509/1‑251 (MQ=255)                                                                                                                                            cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagct                                                                                                        <  1:263752/251‑1 (MQ=255)                                                                                                                                            cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagct                                                                                                        <  1:8668/251‑1 (MQ=255)                                                                                                                                            cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagc                                                                                                         <  1:849960/250‑1 (MQ=255)                                                                                                                                            cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagc                                                                                                         <  1:582697/250‑1 (MQ=255)                                                                                                                                                    agaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGt                                                                                                                      <  1:658978/229‑1 (MQ=255)                                                                                                                                                    agaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCagt                                                                                                >  1:366663/1‑251 (MQ=255)                                                                                                                                                     gaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTtagaaat                                                                                                                                                                                                                      >  1:665761/1‑132 (MQ=255)                                                                                                                                                     gaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTAtgt                                                                                                                                                                                       >  1:688041/1‑163 (MQ=255)                                                                                                                                                     gaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGc                                                                                                                                                                 <  1:228287/185‑1 (MQ=255)                                                                                                                                                     gaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCcac                                                                                                                                  <  1:156061/216‑1 (MQ=255)                                                                                                                                                      aTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCagtag                                                                                              <  1:327095/251‑1 (MQ=255)                                                                                                                                                      aTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCagtag                                                                                              <  1:206439/251‑1 (MQ=255)                                                                                                                                                           ggCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTAtt                                                                                                                                                                                <  1:106354/164‑1 (MQ=255)                                                                                                                                                           ggCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTc                                                                                                                           <  1:533619/217‑1 (MQ=255)                                                                                                                                                           ggCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTg                                                                                         >  1:666792/1‑251 (MQ=255)                                                                                                                                                              aaTAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCtt                                                                                      <  1:705330/251‑1 (MQ=255)                                                                                                                                                                   ccGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCg                                                                                 >  1:606845/1‑251 (MQ=255)                                                                                                                                                                       tATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTtat                                                                                                                                                                            <  1:357189/156‑1 (MQ=255)                                                                                                                                                                       tATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGt                                                                                                                      <  1:57236/210‑1 (MQ=255)                                                                                                                                                                        aTTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAAt                                                                                                                                                                                                        >  1:788872/1‑127 (MQ=255)                                                                                                                                                                                 gTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTc                                                                                                                                                                                                     <  1:77343/121‑1 (MQ=255)                                                                                                                                                                                        tCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACg                                                            <  1:822048/251‑1 (MQ=255)                                                                                                                                                                                                      cAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTc                                               <  1:617294/250‑1 (MQ=255)                                                                                                                                                                                                           cAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAg                                                                       <  1:543361/221‑1 (MQ=255)                                                                                                                                                                                                                                 tcCCCAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACtatta                                                  >  1:159691/1‑220 (MQ=255)                                                                                                                                                                                                                                  ccccAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTc                                               >  1:184892/1‑222 (MQ=255)                                                                                                                                                                                                                                  ccccAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGAt                  >  1:411878/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccccAAACCGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGAt                  >  1:394094/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccGGTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTg                                                                                                                 <  1:268376/149‑1 (MQ=255)                                                                                                                                                                                                                                           ggTAAAATGTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACt                                                      >  1:516975/1‑206 (MQ=255)                                                                                                                                                                                                                                                   gTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGcc                                                                                                                                    <  1:462425/120‑1 (MQ=255)                                                                                                                                                                                                                                                   gTCTATAA‑TTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGt  >  1:529695/1‑250 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   CGCCTTAGCGATTGCAAAGTTAGAAAATCTGGAATTAACGGATGCCCATTGGGAAATTATCCATTTTGTGCGTGATTTTTATCAAGAATATAAAACCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGT  >  1/1775284‑1775776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑