Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,613,326	Δ2 bp	intergenic (‑168698/‑28437)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,613,324	0	C	.	100.0%	146.3 / NA	35	intergenic (‑168696/‑28440)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/20);  total (15/20)
*	1	1,613,325	0	T	.	100.0%	146.3 / NA	35	intergenic (‑168697/‑28439)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/20);  total (15/20)

AACGTTTTGAAGACGGCAAAGTGCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGCTCTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATCTTACTTAATATTACAAATTCTATCGCTATTAAATCAATGTGCCAAAATCGGGTACGAAAATTTCACATTAAATAATGCAGAGTATCTTCA  >  1/1613074‑1613525                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                         aaCGTTTTGAAGACGGCAAAGTGCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGc                                                                                                                                                                                                           <  1:517162/251‑1 (MQ=255)        tGAAGACGGCAAAGTGCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTATCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGcttttatt                                                                                                                                                                                                    <  1:422995/251‑7 (MQ=255)        tGAAGACGGCAAAGTGCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGcttttatt                                                                                                                                                                                                    <  1:813742/251‑7 (MQ=255)          aaGACGGCAAAGTGCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTAc                                                                                                                                                                                                <  1:508291/251‑1 (MQ=255)                       gCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAAt                                                                                                                                                                                   >  1:550682/1‑251 (MQ=255)                               aaGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGc                                                                                                                                                                           <  1:470362/251‑1 (MQ=255)                                      gttAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGGGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGc                                                                                                                                                                                                           <  1:27151/214‑1 (MQ=255)                                       ttAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGa                                                                                                                                                                    <  1:233936/250‑1 (MQ=255)                                       ttAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGAt                                                                                                                                                                   <  1:866046/251‑1 (MQ=255)                                       ttAAAAGATCTCTTAAACGCTTACAAAGCCGGCTTAAAAACCCTCTACTACCAGAACGCCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGCGCCGGTGGGGCGTGTAAGATTTAATTATTAATAAACCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTGCCTGGCATTATAATTACAATGCTTATAGAt                                                                                                                                                                   <  1:411904/251‑1 (MQ=255)                                               tctcTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGc                                                                                                                                                                                                           <  1:636196/205‑1 (MQ=255)                                               tctcTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATaa                                                                                                                                                                                    >  1:86015/1‑226 (MQ=255)                                                  ctTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGa                                                                                                                                                        <  1:27801/251‑1 (MQ=255)                                                          cTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAg                                                                                                                                                                     <  1:290620/230‑1 (MQ=255)                                                               aaaTACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATgg                                                                                                                                           <  1:106131/251‑1 (MQ=255)                                                                  tACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAAt                                                                                                                                        <  1:897557/251‑1 (MQ=255)                                                                  tACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAAt                                                                                                                                        <  1:125055/251‑1 (MQ=255)                                                                      gCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACaa                                                                                                                                                                              <  1:781173/209‑1 (MQ=255)                                                                      gCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATaa                                                                                                                                      >  1:888470/1‑249 (MQ=255)                                                                       cTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACta                                                                                                                                   <  1:27242/251‑1 (MQ=255)                                                                         tAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGaa                                                                                                                                              >  1:371470/1‑238 (MQ=255)                                                                             aaCCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAata                                                                                                                             >  1:446409/1‑251 (MQ=255)                                                                             aaCCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAata                                                                                                                             <  1:808074/251‑1 (MQ=255)                                                                              aCCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTAc                                                                                                                                                                                                >  1:337571/1‑183 (MQ=255)                                                                                   ctactaCCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCAtt                                                                                                                       >  1:876021/1‑251 (MQ=255)                                                                                               cacCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATCAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGACATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAata                                                                                                                             >  1:437634/1‑233 (MQ=255)                                                                                                                              gaTTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGa                                                                                                                                                                    <  1:737998/163‑1 (MQ=255)                                                                                                                                         tgGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGa                                                                                                                                                                    <  1:366115/152‑1 (MQ=255)                                                                                                                                                        ggCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAata                                                                                                                             >  1:459502/1‑176 (MQ=255)                                                                                                                                                                 gATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATCTTACTTAATATTACAAATTCTATCGCTATTAAAt                                                          <  1:752100/234‑1 (MQ=255)                                                                                                                                                                          attaATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATaa                                                                                                                                                                                    >  1:757054/1‑103 (MQ=255)                                                                                                                                                                             aataatCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGAt                                                                                                                                                                   <  1:293207/117‑1 (MQ=255)                                                                                                                                                                                       cctcATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGa                                                                                                                                                                    >  1:237917/1‑106 (MQ=255)                                                                                                                                                                                         tcATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTAc                                                                                                                                                                                                >  1:85423/1‑76 (MQ=255)                                                                                                                                                                                          cATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATCTTACTTAATATTACAAATTCTATCGCTATTAAATc                                                         >  1:13602/1‑210 (MQ=255)                                                                                                                                                                                               gAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATCTTACTTAATATTACAAATTCTATCGCTATTAAATCAATGTGCCAAAATCGGGTACGAAAATTTCACATTAAATAATACag            <  1:185737/250‑1 (MQ=255)                                                                                                                                                                                                   gattagattaCTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATaa                                                                                                                                                                                    >  1:150574/1‑78 (MQ=255)                                                                                                                                                                                                         attaCTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATCTTACTTAATATTACAAATTCTATCGCTATTAAATCAATGTGCCAAAATCGGGTACGAAAATTTCACATTAAATAATACAGAGTATCTTCa  <  1:173441/250‑1 (MQ=255)                                                                                                                                                                                                             ctctTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGc                                                                                                                                                                           <  1:402478/77‑1 (MQ=255)                                                                                                                                                                                                                       gTTCATATGAATGAATTGACAATAAAAATTGTAATG‑‑CTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATct                                                                                           >  1:460986/1‑147 (MQ=255)                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                         AACGTTTTGAAGACGGCAAAGTGCCGATGAAAGTGTTGTTAAAAGATCTCTTAACCGCTTACAAATACGGCTTAAAAACCCTCTACTACCAGAACACCCGCGACGGCGCCGAAGACAGCCAAGAAGATTTGGATGATGGTTGTGCCGGTGGGGCGTGTAAGATTTAATTATTAATAATCCCTCCTCATTGGAGGGATTAGATTACTCTTAGCGAGTTCATATGAATGAATTGACAATAAAAATTGTAATGCTCTTTTATTACCTGGCATTATAATTACAATGCTTATAGATAAGTTCACTGAACATAAAGAATGGAATAACTATAAATATTCATTGTTTGTTATTTCATATGGAATATTATCTTACTTAATATTACAAATTCTATCGCTATTAAATCAATGTGCCAAAATCGGGTACGAAAATTTCACATTAAATAATGCAGAGTATCTTCA  >  1/1613074‑1613525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑