Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,535,369	(A)5→4	intergenic (‑90741/‑106395)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,535,365	0	A	.	100.0%	167.0 / NA	40	intergenic (‑90737/‑106399)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/19);  total (21/19)

CCGTTTGTTCATGCCCGAATTTCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGGAAAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGAAAGGCACGTTAAACGCATGGCAAACTTG  >  1/1535133‑1535608                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    ccGTTTGTTCATGCCCGAATTTCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAgtg                                                                                                                                                                                                                                  <  1:688245/251‑1 (MQ=255) ccGTTTGTTCATGCCCGAATTTCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAgtg                                                                                                                                                                                                                                  <  1:556593/251‑1 (MQ=255)    tttGTTCATGCCCGAATTTCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAgtgtgt                                                                                                                                                                                                                               <  1:323036/251‑1 (MQ=255)               ccGAATTTCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAgg                                                                                                                                                                                                                    <  1:558605/251‑1 (MQ=255)                      tCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGCAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGa                                                                                                                                                                                                                            >  1:449215/1‑236 (MQ=255)                      tCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCcg                                                                                                                                                                                                              >  1:353841/1‑250 (MQ=255)                       cACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAgtgtgt                                                                                                                                                                                                                               <  1:399936/232‑1 (MQ=255)                                                   tcctTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATg                                                                                                                                                                                <  1:899805/251‑1 (MQ=255)                                                      ttGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCag                                                                                                                                                                             <  1:42589/251‑1 (MQ=255)                                                             gTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGaaa                                                                                                                                                                      >  1:803460/1‑251 (MQ=255)                                                              ttCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAggg                                                                                                                                                                         >  1:119592/1‑247 (MQ=255)                                                               tCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAAt                                                                                                                                                                    <  1:419222/251‑1 (MQ=255)                                                                 cAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGttt                                                                                                                                                                                                                 >  1:483511/1‑204 (MQ=255)                                                                            tgtgAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTc                                                                                                                                                       <  1:890978/251‑1 (MQ=255)                                                                                        cAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAAc                                                                                                                                           <  1:188965/251‑1 (MQ=255)                                                                                         aaCGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACa                                                                                                                                          <  1:123613/251‑1 (MQ=255)                                                                                                 ccGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGAc                                                                                                                                   <  1:719627/250‑1 (MQ=255)                                                                                                        aaGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGt                                                                                                                           <  1:354812/251‑1 (MQ=255)                                                                                                              ggTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAGTCAAGTGTTTTTTTGAGAAAAATAAGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGc                                                                                                                                                                               >  1:8933/1‑193 (MQ=255)                                                                                                                         cGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGc                                                                                                           <  1:409595/250‑1 (MQ=255)                                                                                                                                                        ccTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACatat                                                                           >  1:631227/1‑251 (MQ=255)                                                                                                                                                         cTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATAtt                                                                          <  1:898497/251‑1 (MQ=255)                                                                                                                                                                      aaaTTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCg                                                             >  1:77219/1‑251 (MQ=255)                                                                                                                                                                                ccATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGGCGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCgg                                                   >  1:594628/1‑251 (MQ=255)                                                                                                                                                                                     ataaAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAAt                                               >  1:437329/1‑250 (MQ=255)                                                                                                                                                                                     ataaAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATg                                              >  1:169152/1‑251 (MQ=255)                                                                                                                                                                                         aaTATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGcc                                                           <  1:521132/234‑1 (MQ=255)                                                                                                                                                                                            atGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGaaa                                                >  1:400788/1‑242 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACata                                                                            <  1:71232/205‑1 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGgcg                                           >  1:852568/1‑238 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGa                              >  1:642534/1‑251 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGa                              >  1:750700/1‑251 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGa                              >  1:263803/1‑251 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGa                              >  1:895956/1‑251 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATCAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGa                              >  1:372556/1‑251 (MQ=255)                                                                                                                                                                                                     cTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGCAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGcc                                                           >  1:361468/1‑222 (MQ=255)                                                                                                                                                                                                                  tgtTTTTTTGAGAAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGAAAGGCGCGTTaaa                 <  1:806926/251‑1 (MQ=255)                                                                                                                                                                                                                           gagaAAAATACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGAAAGGCGCGTTAAACGCATGGCa        >  1:564412/1‑251 (MQ=255)                                                                                                                                                                                                                               aaaaTACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCACCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGAAAGGCGCGTTAAACGCATGGCAAACt    <  1:286361/251‑1 (MQ=255)                                                                                                                                                                                                                                  aTACGGG‑AAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGAAAGGCGCGTTAAACGCATGGCAAACTTg  >  1:469596/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    CCGTTTGTTCATGCCCGAATTTCACTACATATTGCCCGGTAGAATTCGCCTCCTTGACGGGTTCCAGCCATACGTTGTGAGCGTTAGCAACGGATGCCGCGAAAAGTGCGGTCAAAATAGCGGTTGTTTTTTCGAATTTCATGTGATCTCTCCTTTTATCAATTAAAATTTGATACCATAATAAAATATGAAGTAACTTTAGAATCAAGTGTTTTTTTGAGAAAAATACGGGAAAAATGAGAGAGATAAGTGTGTGGATAATTAGGTTTCCGCGTACGGCAAACGCGTACGCGGCGGGAATGCAGAGGGAAAATAAACCGTTAATTCAACTCCGCCAACATGGCGACAACCATGTCGGACGATTGTTTGGCAGCGAGCGGTAGAAATTCTTCAAAAGACATATTAGCTTTGCCATCGCCCGCATCGGAAATGGCGCGCACCACCACGAAAGGCACGTTAAACGCATGGCAAACTTG  >  1/1535133‑1535608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑