Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,484	Δ1 bp	intergenic (‑38856/‑158280)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,484	0	C	.	100.0%	207.2 / NA	50	intergenic (‑38856/‑158280)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/18);  total (32/18)

GCCCGCATCAACTATCAAGTCATAGCCGACAGAAAGTGCAGTCGGTTGCGCGCCCAAGCTCCAGACAAAACAGTCGGTCGGCTTGATAAATGCGCGCTCCGCGTCCGATAGCACAAGCGGGAGTACGTGATATGGCACCTCAAACAAG‑CGCGCGGAATCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGTCGGGCAGATTAACCTGGATAACATCACCC  >  1/1483340‑1483697                                                                                                                                                 |                                                                                                                                                                                                                          acccacattaaccagcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCa                                                                                                                <  1:851526/232‑1 (MQ=255)             cagcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCAC‑G‑TCGcc                                                                                                   >  1:885890/8‑250 (MQ=255)               gcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTGCCGGTAACATCCTGCAAGCAC‑G‑GCGCcac‑a                                                                                               >  1:123973/6‑251 (MQ=255)               gcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAATCCTGCCAGCAC‑G‑TCGCcac‑a                                                                                               >  1:336286/6‑251 (MQ=255)                catatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCATGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCcac                                                                                                 <  1:431013/245‑1 (MQ=255)                  tatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAgcgg                                                                                                                                        >  1:87667/3‑210 (MQ=255)                  tatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAATCCTGCAAGCAC‑C‑TCGCCCC‑AAt                                                                                             >  1:493069/3‑250 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAc                                                                                                                        <  1:587443/224‑1 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCCC‑AAt                                                                                             >  1:792839/2‑249 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCAC‑G‑TCGCCCC‑AAt                                                                                             >  1:330367/2‑249 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCCTAAACATCCTTCCAGCAC‑G‑TCGCcaccaac                                                                                             >  1:685481/2‑246 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCAAACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCAAGCCGTAAACATCCTGCAAGCa                                                                                                            >  1:114049/2‑237 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGagtag                                                                                                                                               >  1:715757/1‑201 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTa                                                                                                                                            <  1:7335/204‑1 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTTGCGTGAACATCCTGCAAggccggctccccaaact                                                                                              >  1:429749/1‑234 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AAtct                                                                                           >  1:534190/1‑250 (MQ=255)                         gcccaccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCa                                                                                                            <  1:251048/224‑1 (MQ=255)                             accgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGGATGCCGTAAAAATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCCCGtt                                                                                 >  1:616503/4‑251 (MQ=255)                              ccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTg                                                                                <  1:736308/249‑1 (MQ=255)                              ccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAAACCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCCCGTTg                                                                                >  1:224377/3‑251 (MQ=255)                                 aaagcgctgTCGGCTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTg                                                                                                                                                    <  1:446435/175‑1 (MQ=255)                                         gTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGCGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGTCGTAAACATCCTGCAAGAAC‑G‑GCGACACAAATC‑CGCCCCCGTTTCCGTTTTTaca                                                                     >  1:507157/1‑249 (MQ=255)                                             gTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCCAGCAC‑G‑TCGCCCC‑AATCTCGCCCCCGTTGCCGTTTTTAAGCTc                                                                  >  1:524924/1‑250 (MQ=255)                                                   cgcCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTgtgt                                                                                                                                                      >  1:750627/1‑163 (MQ=255)                                                     cccAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAg                                                                                                                                                                        <  1:650996/143‑1 (MQ=255)                                                     cccAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AAACTCGCCCCCGTTGCCGGTTTTAAGCTCCCCAAccc                                                          >  1:575099/1‑248 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAac                                                     <  1:270186/250‑1 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAac                                                     <  1:294858/250‑1 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAAAATCCTGCAAGCAC‑G‑TCGCCAC‑aaac                                                                                            >  1:399696/1‑209 (MQ=255)                                                             ccataCAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCAC‑G‑TCGCCCC‑AAACTCGCCCCCGTTGCCGGTTTTTAGCTCCCCAACTCGGAAcacca                                                 >  1:816003/5‑251 (MQ=255)                                                               ataCAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAc                                                                                                                        >  1:246106/3‑181 (MQ=255)                                                                tacaaaccaatccgtcggCTTAATAACCGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGt                                              <  1:307964/238‑1 (MQ=255)                                                                    aaaCAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATTCCGTAAAAATCCCGCAAGCAC‑G‑GCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACCTcaa                                           >  1:616932/1‑250 (MQ=255)                                                                    aaaCAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AAACTCGCCCCCGTTGCCGCTTTTAAACTCCCCAACTCCGAACACCACCTcaa                                           >  1:415614/1‑250 (MQ=255)                                                                       caatccgtcggCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAat                                       <  1:863034/245‑1 (MQ=255)                                                                         atccgtcggCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAatat                                     <  1:477199/247‑1 (MQ=255)                                                                                ggCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTGCCGTAAACATCCTCCAAGCAC‑G‑TCGCCCC‑AATCTCGCCCCCGTTGCCGGTTTTTAGCTCCCCCACTCGGAACACCACGTCAACAATATCGCggg                               >  1:128418/1‑249 (MQ=255)                                                                                  cttaaTAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGGTTTTAAGCTCCCCAACTCGGAACACCCCGTCAACAATATCGCGGTCgg                            >  1:205158/5‑251 (MQ=255)                                                                                         aacgcacgctcggcatcggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCCACTCGGAACACCACGGcaacaaaat                                     >  1:838062/19‑232 (MQ=255)                                                                                                     gcatcggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGcc                                                                                                                                                               >  1:173238/7‑104 (MQ=255)                                                                                                     gcatcggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGATAACATCCTTCAAGCAC‑G‑TCGCCAC‑GATCTCGCCCTCGTTTACGTTTTTATGCTCCCCCAATCTGTACACCACGTTCACAAAATCGCGGTCGGGCAGGTTAAcccgcgtaaa         >  1:857370/7‑243 (MQ=255)                                                                                                       atcggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGTCGGGCAGATTAACCCGGATAACAt       <  1:84447/247‑1 (MQ=255)                                                                                                         cggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCCGCCGTAAAAATCCTGCCA‑CACAGATC‑CCAC‑AATCTGGCCCCTGCTGCCGCTTTTAAGTTCCCCCAATCTGAACACCATGTCTACAATATCGCGGGTGGGGAGATTaatccggggtttata      >  1:706407/3‑237 (MQ=255)                                                                                                          ggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCCC‑CATCTCGCCCCCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCCACAATATCGCGGTCGGGCAGATTAACCCGGATAACATCa     >  1:138415/2‑250 (MQ=255)                                                                                                          ggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAat                                       <  1:10003/215‑1 (MQ=255)                                                                                                          ggATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGGCGGGCAGATTAACCCGGATAACATCa     >  1:273874/2‑250 (MQ=255)                                                                                                           gATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCCC‑AAACTCGCCCTCGTTGCCGTTTTTTAGCTCCCCAACTCGGAACACCACGTCAACAATATCCCGGTCGGGCAGATTAACCCGGATAACATCAc    >  1:430407/1‑250 (MQ=255)                                                                                                            aTAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGTCGGGCAGATTAACCCGGATAACATCAccc  <  1:787988/251‑1 (MQ=255)                                                                                                            aTAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGTCGGGCAGATTAACCCGGATAACATCAccc  <  1:704171/251‑1 (MQ=255)                                                                                                            aTAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGTCGGGCAGATTAACCCGGATAACATCAcc   <  1:312338/250‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                                                                                          GCCCGCATCAACTATCAAGTCATAGCCGACAGAAAGTGCAGTCGGTTGCGCGCCCAAGCTCCAGACAAAACAGTCGGTCGGCTTGATAAATGCGCGCTCCGCGTCCGATAGCACAAGCGGGAGTACGTGATATGGCACCTCAAACAAG‑CGCGCGGAATCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCAC‑G‑TCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGTCGGGCAGATTAACCTGGATAACATCACCC  >  1/1483340‑1483697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑