| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,034,999 | Δ1 bp | intergenic (‑28543/‑220) | unknown ← / → unknown | tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,034,999 | 0 | G | . | 86.2% | 54.7 / 11.7 | 29 | intergenic (‑28543/‑220) | unknown/unknown | tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base . (12/13); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGTGCGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAACTCTTGCAAAG‑AATTAGTAAATACCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCGTTTATTTATATTATTTATAATAGATTAATTAAGTCAGCTATTGAGGGATTAAGCTTTTTGAATTGAAGAGTTT > 1/1034777‑1035235 | gtggtgCGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAacg > 1:198120/1‑251 (MQ=38) gtgCGGCACTTAGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAacgacg > 1:421433/1‑251 (MQ=38) cGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACgcgc < 1:169811/251‑1 (MQ=38) cGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAggg < 1:77441/251‑1 (MQ=37) cGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCA‑TTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAggg < 1:554485/250‑1 (MQ=37) ttGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAg < 1:107148/251‑1 (MQ=37) gAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAggg > 1:806376/1‑240 (MQ=37) gAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGCGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACa > 1:532233/1‑251 (MQ=25) aaaTAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTtat > 1:867558/1‑232 (MQ=25) aaaTAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGtttttt > 1:708186/1‑251 (MQ=25) atttTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGAc > 1:797658/1‑227 (MQ=25) atttTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCA‑TTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTaa > 1:68217/1‑250 (MQ=21) aaaGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACaa < 1:746276/250‑1 (MQ=21) aaGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAAc < 1:896755/251‑1 (MQ=21) ccAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACtg < 1:314764/250‑1 (MQ=18) cAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACtgtg < 1:476513/251‑1 (MQ=18) cAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACaa > 1:550647/1‑233 (MQ=18) aaaTTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGAt < 1:43455/251‑1 (MQ=18) aaaTTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGAt < 1:30888/251‑1 (MQ=18) gCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAaatg < 1:793014/241‑2 (MQ=14) cATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGAt > 1:205842/1‑228 (MQ=17) tttttGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGATTGaa > 1:803828/1‑250 (MQ=14) aTCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCg > 1:306897/1‑251 (MQ=11) tctcAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTtat < 1:716553/137‑1 (MQ=11) tctcAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGA‑TAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGAt < 1:764852/223‑1 (MQ=11) ctTGCAAAG‑AATTAGTAAATACCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTGATAGGTGCCGGAAGATTCGTTTATTTATATTATTTATAATAGATTAATTAAGTCAGCTATTGAGGGATTAAGCTTTTTGAATTGAAGAGttt > 1:284246/1‑251 (MQ=14) g‑AATTAGTAAATACCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGa > 1:26632/1‑163 (MQ=32) g‑AATTAGTAAATACCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGAAg < 1:35627/165‑1 (MQ=32) g‑AATTAGTAAATACCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCGTTTATTTATATTATTTataat > 1:322458/1‑191 (MQ=32) | GTGGTGCGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAACTCTTGCAAAG‑AATTAGTAAATACCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCGTTTATTTATATTATTTATAATAGATTAATTAAGTCAGCTATTGAGGGATTAAGCTTTTTGAATTGAAGAGTTT > 1/1034777‑1035235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |