Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,742	2 bp→TA	coding (411‑412/888 nt)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,742	0	A	T	100.0%	119.4 / NA	43	S137S (TCA→TCT)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/21);  total (22/21)
*	1	1,484,743	0	G	A	100.0%	113.9 / NA	43	G138S (GGT→AGC)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)

AATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTA  >  1/1484539‑1484959                                                                                                                                                                                                            ||                                                                                                                                                                                                                         aaTATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                                            <  1:227566/251‑4 (MQ=255) aaTATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                                            <  1:742711/251‑4 (MQ=255)       gTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCa                                                                                                                                                                      >  1:405534/1‑251 (MQ=255)       gTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCa                                                                                                                                                                      >  1:288970/1‑251 (MQ=255)            aaGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAg                                                                                                                                                                 >  1:861787/1‑251 (MQ=255)                 gttTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTa                                                                                                                                                                                      <  1:604861/225‑1 (MQ=255)                                                    gCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctat                                                                                                                            >  1:597387/1‑235 (MQ=255)                                                      ttATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagggg                                                                                                                       <  1:567090/251‑19 (MQ=255)                                                         tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCgg                                                                                                                                                                                        <  1:263360/183‑1 (MQ=255)                                                         tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggagg                                                                                                                    >  1:281931/1‑230 (MQ=255)                                                         tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggagg                                                                                                                    >  1:691957/1‑230 (MQ=255)                                                           ttCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                                            <  1:778425/193‑4 (MQ=255)                                                             cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTa                                                                                                                                                                  <  1:3738/201‑1 (MQ=255)                                                             cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCAt                                                                                                                                          <  1:582068/225‑1 (MQ=255)                                                             cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggaggacc                                                                                                                 >  1:604411/1‑226 (MQ=255)                                                             cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggaggacc                                                                                                                 >  1:251184/1‑226 (MQ=255)                                                             cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggagg                                                                                                                    <  1:871446/247‑22 (MQ=255)                                                             cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATa                                                                                                                                         <  1:109575/226‑1 (MQ=255)                                                                       gATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAggcg                                                                                                                                                              <  1:142468/195‑3 (MQ=255)                                                                       gATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagg                                                                                                                         <  1:1051/232‑17 (MQ=255)                                                                       gATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctat                                                                                                                            >  1:515490/1‑216 (MQ=255)                                                                            gtgtTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagggg                                                                                                                       >  1:499845/1‑211 (MQ=255)                                                                            gtgtTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagac                                                                                                                                      <  1:622235/214‑4 (MQ=255)                                                                                                tgttgGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                                            >  1:391947/1‑153 (MQ=255)                                                                                                          gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAAc                                                                                                                                                                                               >  1:375250/1‑127 (MQ=255)                                                                                                          gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatag                                                                                                                          >  1:504647/1‑181 (MQ=255)                                                                                                          gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGa                                                                   >  1:816286/1‑251 (MQ=255)                                                                                                                               ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                                                    <  1:482687/229‑1 (MQ=255)                                                                                                                               ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGATGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTAt                                              >  1:238451/1‑251 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTaa                                                                                                                                                                                     >  1:12792/1‑111 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagg                                                                                                                         <  1:335149/171‑17 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                                                    >  1:771087/1‑224 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                                                    <  1:513144/224‑1 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGag                                                                  >  1:617992/1‑225 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                                          >  1:290066/1‑250 (MQ=255)                                                                                                                                    gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                                          >  1:579626/1‑250 (MQ=255)                                                                                                                                     tACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTAc                                         <  1:271444/250‑1 (MQ=255)                                                                                                                                            ataaAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAAc                                                 >  1:549062/1‑235 (MQ=255)                                                                                                                                              aaaaaaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCActaattt                               <  1:678777/251‑7 (MQ=255)                                                                                                                                                  aaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTaaac                           <  1:351924/251‑3 (MQ=255)                                                                                                                                                                     tccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGc                                       <  1:408478/216‑1 (MQ=255)                                                                                                                                                                      ccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATAcggga       <  1:393986/248‑4 (MQ=255)                                                                                                                                                                           ggTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTa  >  1:842261/1‑251 (MQ=255)                                                                                                                                                                                                            ||                                                                                                                                                                                                                         AATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTA  >  1/1484539‑1484959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑