Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,532	2 bp→AG	coding (201‑202/888 nt)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,532	0	G	A	100.0%	49.6 / NA	20	L67L (TTG→TTA)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)
*	1	1,484,533	0	A	G	100.0%	52.8 / NA	20	I68V (ATT→GTT)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

GAGTGCTGAACTACAAAGTATCTTCGGAGAAGCTGAGTAATGAATTATTATTTGTATTATGCCGTCCTAACAATAGGTGGGCTGTTATTTATATATACCTTAAGTAGTTGGTTTTCCACTAAAAGAAAAATTAATGAGTATGATACCTCTGTAAGTAGAACAACAAGTTTTTTTAAGAGCCTCAAATCAAAAATTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAGAAACTTGATTCGCAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTG  >  1/1484293‑1484746                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                      gagtactgagttACAAAGTATTTTCGGAGAAGCTGAGTGATGAGCCATTATTTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAatat                                                                                                                                                                                                             >  1:68201/12‑251 (MQ=255)    tactgagttACAAAGTATTTTCGGAGAAGCTGAGTGATGAGCCATTATTTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTg                                                                                                                                                                                                           <  1:84604/242‑1 (MQ=255)                                gctgagtgatgagccattattTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTtata                                                                                                                                                                                                      >  1:660140/16‑227 (MQ=255)                                     gtgatgagccattattTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGt                                                                                                                                                                         >  1:795947/11‑251 (MQ=255)                                                                    ttaCAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGac                                                                                                                                             >  1:414867/3‑247 (MQ=255)                                                                                             atataCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGt                                                                                                                                                                <  1:299468/204‑1 (MQ=255)                                                                                                taCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCttgttg                                                                                                              <  1:657485/251‑1 (MQ=255)                                                                                                 aCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAgt                                                                                                                                                                                                   <  1:431997/165‑1 (MQ=255)                                                                                                 aCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTgg                                                                                                             >  1:192908/1‑251 (MQ=255)                                                                                                                                          gTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGt                                                                                                                                                                >  1:482514/1‑159 (MQ=255)                                                                                                                                                   ctctATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCatat                                                                                                                                                 <  1:897551/165‑1 (MQ=255)                                                                                                                                                   ctctATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAgtgt                                                                                                                                   <  1:492030/179‑1 (MQ=255)                                                                                                                                                   ctctATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGtaa                                                                      >  1:420882/1‑240 (MQ=255)                                                                                                                                                    tctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTc                                                                                                                                                     >  1:831896/5‑160 (MQ=255)                                                                                                                                                    tctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCatat                                                                                                                                                 >  1:604747/5‑164 (MQ=255)                                                                                                                                                     ctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACt                                                                                     >  1:726634/4‑223 (MQ=255)                                                                                                                                                                        tttttttAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAgg                                      <  1:225040/251‑1 (MQ=255)                                                                                                                                                                                                                    aCTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTctagcg  <  1:484854/243‑6 (MQ=255)                                                                                                                                                                                                                     cTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAgg                                      >  1:255825/1‑206 (MQ=255)                                                                                                                                                                                                                       ttACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAgtgt                                                                                                                                   <  1:785880/111‑1 (MQ=255)                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                      GAGTGCTGAACTACAAAGTATCTTCGGAGAAGCTGAGTAATGAATTATTATTTGTATTATGCCGTCCTAACAATAGGTGGGCTGTTATTTATATATACCTTAAGTAGTTGGTTTTCCACTAAAAGAAAAATTAATGAGTATGATACCTCTGTAAGTAGAACAACAAGTTTTTTTAAGAGCCTCAAATCAAAAATTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAGAAACTTGATTCGCAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTG  >  1/1484293‑1484746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑