| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,484,134 | 2 bp→AA | coding (1083‑1084/1281 nt) | unknown → | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,484,134 | 0 | G | A | 100.0% | 25.2 / NA | 11 | E361E (GAG→GAA) | unknown | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/3); total (8/3) | |||||||||||
| * | 1 | 1,484,135 | 0 | G | A | 90.9% | 19.2 / ‑4.1 | 11 | V362I (GTT→ATA) | unknown | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (7/3); total (8/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGAAGCATTCCAAATGTTACAAGCAATGAATACCGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATAGCCCTCGCGATGCAACATCTCGCTTGGAAAGTATGGTTATGATGTCGAATGCATCCCTACCGTTAGAGGCAATTCGCCGTAATATCTCTTCAGCAGTAAACATAATAGTGCAGGCGTCCCGTTTAAATGATGGTTCAAGAAAAATTATGAATATCACTGAGGTTATGGGAATGGAAAACGGTCAAATCGTTTTACAGGATATATTTTCCTATAAGGCAAGTAAATATCGTGATAAAGAGGGTAAAATTCTTGGTGAGTTTATTAATCACGGACTACTAAC > 1/1483900‑1484253 || tggAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGaaa < 1:462289/251‑1 (MQ=255)tggAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGaa < 1:676093/250‑1 (MQ=255) gaAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGaaaatgg > 1:390491/1‑248 (MQ=255) ttCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAAt > 1:145787/1‑251 (MQ=255) ttCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAAt > 1:775030/1‑251 (MQ=255) aacactggTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTc < 1:401941/245‑1 (MQ=255) cGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCaa > 1:105586/1‑251 (MQ=255) cGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCaa > 1:833597/1‑251 (MQ=255) ggTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAGAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCAAGTaa > 1:438528/1‑251 (MQ=255) ggTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCAAGTaa > 1:323313/1‑251 (MQ=255) ggTTATGATGTCGAATGCATCCCTACCGTTGGAGGCAATTCGCCGTAATATTTCTTCAGCAGTAAACATAATAGTGCAGGCGTCCCGCTTAAACGATGGTTCAAGAAAAATTATGAATATCACTGAAGTTATGGGAATGGAAAACGGTCAAATCGTTTTGCAAGATATATTTTCCTATAAGGCAAGTAAATATCGTGATAAAGAGGGTAAAATTCTTGGCGAGTTTATTAATCACGGACTACTAAc > 1:507049/1‑246 (MQ=255) || TGGAGAAGCATTCCAAATGTTACAAGCAATGAATACCGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATAGCCCTCGCGATGCAACATCTCGCTTGGAAAGTATGGTTATGATGTCGAATGCATCCCTACCGTTAGAGGCAATTCGCCGTAATATCTCTTCAGCAGTAAACATAATAGTGCAGGCGTCCCGTTTAAATGATGGTTCAAGAAAAATTATGAATATCACTGAGGTTATGGGAATGGAAAACGGTCAAATCGTTTTACAGGATATATTTTCCTATAAGGCAAGTAAATATCGTGATAAAGAGGGTAAAATTCTTGGTGAGTTTATTAATCACGGACTACTAAC > 1/1483900‑1484253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |