Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,480,479	2 bp→AC	coding (464‑465/1383 nt)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,480,479	0	G	A	100.0%	114.6 / NA	43	S155N (AGT→AAC)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/17);  total (26/17)
*	1	1,480,480	0	T	C	100.0%	117.1 / NA	43	S155N (AGT→AAC)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/17);  total (26/17)

GTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGTCCCTTAACATCCGATACTGTTAATGTTGATTCTGTTGTGACCAGTATTGCAGACACGAATAAACAACTTAAAAGCCGTTTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAACTTTCTGAGGCAATGGGCATTAACTGGAGCCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAA  >  1/1480245‑1480722                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                   gTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTa                                                                                                                                                                                                                                       >  1:726356/1‑249 (MQ=255)    gAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGaa                                                                                                                                                                                                                                  >  1:818469/1‑251 (MQ=255)     aaCAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGa                                                                                                                                                                                                                                   <  1:80305/249‑1 (MQ=255)                                                                                                                     tttccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttgt                                                                                                                 >  1:567193/4‑251 (MQ=255)                                                                                                                       tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                               <  1:742506/250‑1 (MQ=255)                                                                                                                       tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                               <  1:735964/250‑1 (MQ=255)                                                                                                                       tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                               <  1:591569/250‑1 (MQ=255)                                                                                                                         cAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTagag                                                                                                             <  1:251446/251‑1 (MQ=255)                                                                                                                            aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta                                                                                                          >  1:862888/1‑251 (MQ=255)                                                                                                                            aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta                                                                                                          >  1:795982/1‑251 (MQ=255)                                                                                                                               cTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGtaaaa                                                                                                       >  1:837636/1‑250 (MQ=255)                                                                                                                                      atctGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATaagaaa                                                                                                 >  1:176042/1‑250 (MQ=255)                                                                                                                                          gtgaagaaaGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACttt                                                                                             >  1:216054/7‑250 (MQ=255)                                                                                                                                                   gTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGcgat                                                                                   >  1:244491/1‑248 (MQ=255)                                                                                                                                                      ggtaaGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttg                                                                                                                  <  1:546111/214‑1 (MQ=255)                                                                                                                                                      ggtaaGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATggg                                                                                >  1:781966/4‑251 (MQ=255)                                                                                                                                                           ggcctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTa                                                                           >  1:894327/5‑251 (MQ=255)                                                                                                                                                           ggcctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTa                                                                           >  1:340674/5‑251 (MQ=255)                                                                                                                                                           ggcctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGCTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTa                                                                           >  1:653579/5‑251 (MQ=255)                                                                                                                                                             cctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAAc                                                                         <  1:117591/249‑1 (MQ=255)                                                                                                                                                             cctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAAc                                                                         <  1:226130/249‑1 (MQ=255)                                                                                                                                                              ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                        <  1:878691/250‑1 (MQ=255)                                                                                                                                                              ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                        <  1:159963/250‑1 (MQ=255)                                                                                                                                                              ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                        <  1:237683/250‑1 (MQ=255)                                                                                                                                                              ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                        <  1:806362/250‑1 (MQ=255)                                                                                                                                                                aTGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTgg                                                                      >  1:17735/1‑251 (MQ=255)                                                                                                                                                                aTGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTgg                                                                      >  1:769744/1‑251 (MQ=255)                                                                                                                                                                   tAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAg                                                                    <  1:548782/250‑1 (MQ=255)                                                                                                                                                                       tgaaggtaaaGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTaaagaacggaata                                                               <  1:175643/244‑12 (MQ=255)                                                                                                                                                                         aaggtaaaGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGt                                                              <  1:766454/245‑1 (MQ=255)                                                                                                                                                                                        cacAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                        >  1:40864/3‑225 (MQ=255)                                                                                                                                                                                          cAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCAtt                                            >  1:686430/1‑251 (MQ=255)                                                                                                                                                                                             gaagaaAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                               >  1:361749/1‑181 (MQ=255)                                                                                                                                                                                             gaagaaAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGt                                         >  1:481702/1‑251 (MQ=255)                                                                                                                                                                                              aagaaAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAg                                          <  1:494294/249‑1 (MQ=255)                                                                                                                                                                                                    gCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGt                                  >  1:276557/1‑251 (MQ=255)                                                                                                                                                                                                              tcgtaGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                        <  1:391127/199‑1 (MQ=255)                                                                                                                                                                                                              tcgtaGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGtt                        >  1:647886/5‑251 (MQ=255)                                                                                                                                                                                                                   gAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGa                   >  1:82274/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAAt       >  1:412058/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCAt                                             >  1:305182/1‑211 (MQ=255)                                                                                                                                                                                                                                 gCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATgg     >  1:225218/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGaaa  >  1:430707/1‑251 (MQ=255)                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                   GTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGTCCCTTAACATCCGATACTGTTAATGTTGATTCTGTTGTGACCAGTATTGCAGACACGAATAAACAACTTAAAAGCCGTTTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAACTTTCTGAGGCAATGGGCATTAACTGGAGCCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAA  >  1/1480245‑1480722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑