| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,477,871 | 2 bp→G | intergenic (‑455/‑293) | unknown ← / → unknown | Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,477,871 | 0 | A | . | 90.0% | 29.2 / ‑1.4 | 10 | intergenic (‑455/‑294) | unknown/unknown | Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (4/5); minor base G (1/0); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| * | 1 | 1,477,872 | 0 | T | G | 100.0% | 20.1 / NA | 9 | intergenic (‑456/‑293) | unknown/unknown | Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (4/5); total (4/5) | |||||||||||
GCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATACGTAGTGAATTTATTATTCT‑AAAAAACTTGCATTTTAATTTTTCAGGACTATAATGCCACTCGTTTATTTAATTCCACAAGATTATTA > 1/1477684‑1478089 | gCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAAt < 1:276276/250‑1 (MQ=255) cAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCa > 1:654655/1‑251 (MQ=255) cAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGTGtttttg > 1:438452/1‑153 (MQ=255) taaaTATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAatacg < 1:512730/250‑2 (MQ=255) ttttttAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAc < 1:895687/250‑1 (MQ=255) agttaaAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGttaatt > 1:234983/1‑183 (MQ=255) ttCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatataccggt > 1:232544/1‑243 (MQ=255) agaaaaaaGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatatac < 1:56866/234‑4 (MQ=255) cgcgATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCttatta < 1:354355/251‑1 (MQ=255) tatTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAAt > 1:378869/1‑85 (MQ=255) | GCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATACGTAGTGAATTTATTATTCT‑AAAAAACTTGCATTTTAATTTTTCAGGACTATAATGCCACTCGTTTATTTAATTCCACAAGATTATTA > 1/1477684‑1478089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |