Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,370	2 bp→GG	coding (1707‑1708/1923 nt)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,370	0	A	G	100.0%	161.3 / NA	60	W570R (TGG→CGG)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/24);  total (36/24)
*	1	1,474,371	0	A	G	100.0%	162.2 / NA	60	D569D (GAT→GAC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/24);  total (36/24)

CCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGC  >  1/1474134‑1474587                                                                                                                                                                                                                                             ||                                                                                                                                                                                                                         ccGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTg                                                                                                                                                                                                              <  1:516656/250‑1 (MQ=255)   gTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAg                                                                                                                                                                                                            >  1:152514/1‑250 (MQ=255)        attttGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCa                                                                                                                                                                                                      >  1:332150/5‑251 (MQ=255)                                                 cccTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCa                                                                                                                                                             >  1:679235/1‑251 (MQ=255)                                                 cccTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCa                                                                                                                                                             >  1:785990/1‑251 (MQ=255)                                                    tttCTTCACTTCCAACCGACAATTTGCCAGCCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg                                                                                                                                                          <  1:701517/251‑3 (MQ=255)                                                    tttCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg                                                                                                                                                          >  1:848411/1‑249 (MQ=255)                                                       cttcACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGc                                                                                                                                                              >  1:770914/1‑244 (MQ=255)                                                       cttcACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGaaa                                                                                                                                                       <  1:567562/251‑1 (MQ=255)                                                           aCTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAgg                                                                                                                                                     <  1:242533/249‑1 (MQ=255)                                                            cTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAgggg                                                                                                                                                   >  1:270092/1‑250 (MQ=255)                                                            cTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGc                                                                                                                                                  <  1:779751/251‑1 (MQ=255)                                                                          tttgccaaccACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTaaa                                                                                                                                    <  1:739232/243‑1 (MQ=255)                                                                                 accACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCggg                                                                                                                                                                 >  1:58294/2‑215 (MQ=255)                                                                                     cGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGc                                                                                                                                                              >  1:284703/1‑214 (MQ=255)                                                                                         tttttATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatga                                                                                                                                                         >  1:593913/1‑212 (MQ=255)                                                                                         tttttATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGcccg                                                                                                                     >  1:298862/1‑249 (MQ=255)                                                                                              attttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCATTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCa                                                                                                                >  1:442306/4‑251 (MQ=255)                                                                                                tttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAcc                                                                                                              <  1:896113/250‑1 (MQ=255)                                                                                                tttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAcc                                                                                                              <  1:216956/250‑1 (MQ=255)                                                                                                  tGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCaa                                                                                                            <  1:220851/251‑1 (MQ=255)                                                                                                      tGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGc                                                                                                         >  1:806194/1‑250 (MQ=255)                                                                                                        tcatcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCaa                                                                                                            >  1:537666/1‑245 (MQ=255)                                                                                                         catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTg                                                                                                                                                                              <  1:268539/178‑1 (MQ=255)                                                                                                         catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCg                                                                                                      >  1:750548/1‑250 (MQ=255)                                                                                                         catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCAGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCg                                                                                                      >  1:441831/1‑250 (MQ=255)                                                                                                          atcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCgcc                                                                                                    <  1:673041/251‑3 (MQ=255)                                                                                                              ggacgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtg                                                                                                >  1:890464/7‑249 (MQ=255)                                                                                                                acgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtgc                                                                                               <  1:46631/246‑4 (MQ=255)                                                                                                                acgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtgc                                                                                               <  1:253026/246‑4 (MQ=255)                                                                                                                 cgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg                                                                                                                                                          >  1:745488/4‑188 (MQ=255)                                                                                                                 cgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGccc                                                                                                                      <  1:463500/223‑2 (MQ=255)                                                                                                                   cttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAg                                                                                                                                                                                                            >  1:629123/2‑138 (MQ=255)                                                                                                                    ttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGt                                                                                          >  1:475604/1‑251 (MQ=255)                                                                                                                     tGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGtt                                                                                         >  1:551660/1‑250 (MQ=255)                                                                                                                      gCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGtta                                                                                        >  1:664681/1‑249 (MQ=255)                                                                                                                             gCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCggaca                                                                                 >  1:410682/1‑248 (MQ=255)                                                                                                                                      aaaGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAc                                                                        <  1:332777/251‑1 (MQ=255)                                                                                                                                        aGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAcca                                                                      >  1:616559/1‑249 (MQ=255)                                                                                                                                          gAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAccac                                                                     <  1:457608/250‑4 (MQ=255)                                                                                                                                          gAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAccac                                                                     <  1:214156/250‑4 (MQ=255)                                                                                                                                             aatcctgttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAGCGCCACCACGGaa                                                                 <  1:728512/243‑1 (MQ=255)                                                                                                                                              atcctgttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatga                                                                                                                                                         >  1:229641/8‑159 (MQ=255)                                                                                                                                                   gttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                           >  1:205980/3‑251 (MQ=255)                                                                                                                                                    ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                           <  1:858142/249‑1 (MQ=255)                                                                                                                                                    ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                           <  1:23021/249‑1 (MQ=255)                                                                                                                                                    ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                           <  1:548670/249‑1 (MQ=255)                                                                                                                                                       cccGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGc                                                       <  1:323976/251‑1 (MQ=255)                                                                                                                                                          gCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAc                                                                        >  1:752862/1‑231 (MQ=255)                                                                                                                                                             tactttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAc                                                                                                               >  1:788098/4‑189 (MQ=255)                                                                                                                                                             tactttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGctgcccg                                                 >  1:458958/4‑249 (MQ=255)                                                                                                                                                               ctttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCa                                               >  1:88031/2‑251 (MQ=255)                                                                                                                                                                                                 cGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAAc             <  1:139986/251‑1 (MQ=255)                                                                                                                                                                                                 cGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAAc             <  1:550499/251‑1 (MQ=255)                                                                                                                                                                                                       cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTGGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa         >  1:429372/5‑249 (MQ=255)                                                                                                                                                                                                       cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa         >  1:672984/5‑249 (MQ=255)                                                                                                                                                                                                       cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa         >  1:639961/5‑249 (MQ=255)                                                                                                                                                                                                        ggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAAttgttgttc                                                                                                                           >  1:740332/4‑131 (MQ=255)                                                                                                                                                                                                         gtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACttt     >  1:35151/3‑251 (MQ=255)                                                                                                                                                                                                             gaagaaATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGc  >  1:383121/1‑250 (MQ=255)                                                                                                                                                                                                                                             ||                                                                                                                                                                                                                         CCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGC  >  1/1474134‑1474587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑