| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,135,514 | 2 bp→GG | coding (182‑183/1650 nt) | unknown ← | Type IV secretion system protein VirD4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,135,514 | 0 | T | G | 100.0% | 77.4 / NA | 28 | L61S (TTA→TCC) | unknown | Type IV secretion system protein VirD4 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (16/12); total (16/12) | |||||||||||
| * | 1 | 1,135,515 | 0 | A | G | 100.0% | 76.5 / NA | 28 | L61S (TTA→TCC) | unknown | Type IV secretion system protein VirD4 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/12); total (16/12) | |||||||||||
CGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAATTTCCCAGGATGGCATCCAGCGTAAGAGTAGGGTAGTAACGCTTGAACGCTTCAATCAGACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGAAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAA > 1/1135288‑1135714 || cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa > 1:637904/1‑251 (MQ=255)cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa > 1:561656/1‑251 (MQ=255) tttttgtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAg < 1:878219/229‑1 (MQ=255) ttgtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGa > 1:339015/20‑251 (MQ=255) gtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACa > 1:377257/18‑251 (MQ=255) cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACaaa > 1:175940/15‑250 (MQ=255) cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAAc < 1:102095/237‑1 (MQ=255) cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCCCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAAc < 1:438044/237‑1 (MQ=255) tttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa < 1:820065/245‑1 (MQ=255) tggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc < 1:724521/248‑1 (MQ=255) tggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc < 1:492742/248‑1 (MQ=255) aaaTTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAatat < 1:106068/251‑1 (MQ=255) cGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTACGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCtttt > 1:275186/1‑251 (MQ=255) accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa < 1:322697/218‑1 (MQ=255) accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca > 1:342106/14‑251 (MQ=255) accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca > 1:504395/14‑251 (MQ=255) accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca > 1:665924/14‑251 (MQ=255) ttttttATGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa > 1:839431/2‑219 (MQ=255) ttttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTcatcat > 1:785354/2‑243 (MQ=255) tttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGaaaa < 1:160864/186‑1 (MQ=255) tttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACg > 1:802996/1‑190 (MQ=255) ttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCGCTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct < 1:679974/251‑1 (MQ=255) ttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct < 1:248688/251‑1 (MQ=255) ggttGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc > 1:147112/4‑215 (MQ=255) tGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACtt < 1:294301/171‑1 (MQ=255) tGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGaa > 1:476304/1‑251 (MQ=255) aTAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAATTGGCATCTAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTaa > 1:388841/1‑159 (MQ=255) aTAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAg > 1:797784/1‑191 (MQ=255) || CGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAATTTCCCAGGATGGCATCCAGCGTAAGAGTAGGGTAGTAACGCTTGAACGCTTCAATCAGACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGAAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAA > 1/1135288‑1135714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |