| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,135,348 | 2 bp→GC | coding (348‑349/1650 nt) | unknown ← | Type IV secretion system protein VirD4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,135,348 | 0 | A | G | 100.0% | 94.5 / NA | 36 | L117L (TTA→CTC) | unknown | Type IV secretion system protein VirD4 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/17); total (19/17) | |||||||||||
| * | 1 | 1,135,349 | 0 | A | C | 100.0% | 98.6 / NA | 36 | L116L (CTT→CTG) | unknown | Type IV secretion system protein VirD4 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (19/17); total (19/17) | |||||||||||
GATTAAATCGGTGCGTGTTACGGTCAAACGGGGCAAAGCGATAGACTTTTTGCCCCAATATTTTTTGACGGTATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAA > 1/1135126‑1135538 || gATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATa > 1:281345/1‑251 (MQ=255) tattACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGAATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGAAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGttttttt > 1:611429/3‑246 (MQ=255) cGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtac < 1:410405/251‑8 (MQ=255) cGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgta > 1:71111/1‑244 (MQ=255) atataccttttGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtactt > 1:877799/8‑242 (MQ=255) ccttttGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtacttctgat < 1:163853/249‑15 (MQ=255) aTTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtacttctgattttgtgg > 1:761182/1‑210 (MQ=255) gTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa > 1:332126/1‑251 (MQ=255) tCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa < 1:173188/250‑1 (MQ=255) tCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa < 1:874140/250‑1 (MQ=255) tCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa < 1:53841/250‑1 (MQ=255) aaCCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTa < 1:617744/251‑1 (MQ=255) aCCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaa > 1:185175/1‑250 (MQ=255) aGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctc < 1:459109/250‑6 (MQ=255) gATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa < 1:205277/235‑1 (MQ=255) aTTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctc < 1:118435/248‑6 (MQ=255) gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtacttctgatt > 1:126125/1‑156 (MQ=255) gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactaca > 1:801555/1‑240 (MQ=255) gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactaca > 1:271261/1‑240 (MQ=255) gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTa < 1:830119/240‑1 (MQ=255) tCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTGCCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactacaaa > 1:640863/1‑238 (MQ=255) tCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactac < 1:495319/248‑11 (MQ=255) ccATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactacaaaac < 1:89452/251‑16 (MQ=255) ttGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagac < 1:397716/251‑11 (MQ=255) tAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagactgccat > 1:213856/1‑235 (MQ=255) aTCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagc < 1:806579/214‑4 (MQ=255) tCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagactgccatatttt > 1:37004/1‑229 (MQ=255) tCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagactgccatatttt > 1:30421/1‑229 (MQ=255) cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggag < 1:665929/229‑7 (MQ=255) cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa > 1:637904/1‑251 (MQ=255) cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa > 1:561656/1‑251 (MQ=255) gCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTtttttcttc > 1:381531/1‑105 (MQ=255) gCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcac < 1:703339/210‑8 (MQ=255) gCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaagg < 1:689161/226‑5 (MQ=255) tttACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaa > 1:783674/1‑200 (MQ=255) ccccGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTCTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaa > 1:643501/1‑193 (MQ=255) || GATTAAATCGGTGCGTGTTACGGTCAAACGGGGCAAAGCGATAGACTTTTTGCCCCAATATTTTTTGACGGTATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAA > 1/1135126‑1135538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |