Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	907,496	G→T	intergenic (+488/+17)	unknown → / ← unknown	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	907,496	0	G	T	100.0%	70.8 / NA	28	intergenic (+488/+17)	unknown/unknown	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/10);  total (18/10)

TGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATTCCCCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCGTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTAGCTTTACTTAATAG  >  1/907283‑907674                                                                                                                                                                                                                      |                                                                                                                                                                                   gtgttgaacgatgttgtacatttgatgctaaagtgccctacttgggtatgctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCa                                                                                                                                               >  1:139352‑M2/124‑251 (MQ=255)                   catttgatgctaaagtgccctacttgggtatgctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAatat                                                                                                                                    <  1:663575‑M2/139‑1 (MQ=255)                         atgctaaagtgccctacttgggtatgctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCa                                                                                                                       >  1:51365‑M2/100‑251 (MQ=255)                            ctaaagtgccctacttgggtatgctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACg                                                                                                                     >  1:209946‑M2/97‑250 (MQ=255)                                    ccctacttgggtatgctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGcc                                                                                                            <  1:189809‑M2/163‑1 (MQ=255)                                      ctacttgggtatgctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGccc                                                                                                           >  1:469383‑M2/87‑250 (MQ=255)                                                  gctaattacgtaagagcggtaattatataaaacgaatttaaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTa                                                                                              >  1:267202‑M2/75‑251 (MQ=255)                                                                                        taaataaacagaaaattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAAt                                                                                                                                                                        >  1:521727‑M2/37‑139 (MQ=255)                                                                                                     aattatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCg                                           >  1:353600‑M2/24‑251 (MQ=255)                                                                                                      attatatgaaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTtctc                                                                                                                         <  1:151176‑M2/150‑1 (MQ=255)                                                                                                              aaatggggaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTAt                                                                                                                                          >  1:94481‑M2/15‑147 (MQ=255)                                                                                                                     gaagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACtt                           >  1:534059‑M2/8‑251 (MQ=255)                                                                                                                      aagtatCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTcatcat                                                              >  1:573166‑M2/7‑215 (MQ=255)                                                                                                                                  cGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCtttg               >  1:811968/1‑248 (MQ=255)                                                                                                                                       atttatCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAg                                     <  1:186895/223‑1 (MQ=255)                                                                                                                                       atttatCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTa          >  1:113098/1‑250 (MQ=255)                                                                                                                                             cGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTagta   >  1:787709/1‑248 (MQ=255)                                                                                                                                                                         gAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCtcatca                                                               <  1:558209/163‑1 (MQ=255)                                                                                                                                                                               aaTTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTacac                                                                                                               >  1:454635/1‑109 (MQ=255)                                                                                                                                                                                 ttGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAAttt                                                                             >  1:787692/1‑141 (MQ=255)                                                                                                                                                                                        tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACGCGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAAtt                                                    <  1:844659/159‑1 (MQ=255)                                                                                                                                                                                        tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTAcc                                                                          >  1:374983/1‑137 (MQ=255)                                                                                                                                                                                        tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCtt            <  1:78960/199‑1 (MQ=255)                                                                                                                                                                                         gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTAt                                                                                                                                          >  1:772906/1‑72 (MQ=255)                                                                                                                                                                                         gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTtagtag  <  1:208337/208‑1 (MQ=255)                                                                                                                                                                                             aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCtt            <  1:730566/194‑1 (MQ=255)                                                                                                                                                                                             aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCAATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCt                                   >  1:361858/1‑171 (MQ=255)                                                                                                                                                                                                     ccGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTgggg                                                                                  <  1:468394/116‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                   TGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATTCCCCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCGTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTAGCTTTACTTAATAG  >  1/907283‑907674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑