Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	896,335	A→G	N419N (AAT→AAC)	unknown ←	AmpG permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	896,335	0	A	G	100.0%	206.4 / NA	74	N419N (AAT→AAC)	unknown	AmpG permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/40);  total (34/40)

TAGCAAATTTATTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCATTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATAAACGCCACAAAAGCGGCGGTGCCTAGGCCGACGCCGATATAT  >  1/896094‑896575                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 tAGCAAATTTATTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATggg                                                                                                                                                                                                                                         >  1:858563/1‑251 (MQ=255)    cAAATTTATTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGc                                                                                                                                                                                                                                       <  1:148690/250‑1 (MQ=255)    cAAATTTATTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGc                                                                                                                                                                                                                                       <  1:16912/250‑1 (MQ=255)           aTTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCaaa                                                                                                                                                                                                                               >  1:135546/1‑251 (MQ=255)           aTTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCaaa                                                                                                                                                                                                                               >  1:20202/1‑251 (MQ=255)             tCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAAc                                                                                                                                                                                                                             <  1:509783/251‑1 (MQ=255)                taataaATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAgg                                                                                                                                                                                                                          >  1:213532/1‑251 (MQ=255)                    aaaTTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCaaa                                                                                                                                                                                                                      >  1:525933/1‑251 (MQ=255)                      aTTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCC‑CTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCa                                                                                                                                                                                                                   <  1:609134/251‑1 (MQ=255)                       ttGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCa                                                                                                                                                                                                                   <  1:855847/251‑1 (MQ=255)                            cTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAAc                                                                                                                                                                                                                             <  1:60936/236‑1 (MQ=255)                            cTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATAccc                                                                                                                                                                                                              <  1:108397/251‑1 (MQ=255)                            cTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATAccc                                                                                                                                                                                                              <  1:25198/251‑1 (MQ=255)                            cTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATAcc                                                                                                                                                                                                               >  1:199871/1‑250 (MQ=255)                               gtgtACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAAc                                                                                                                                                                                                                             >  1:285960/1‑233 (MQ=255)                                 gtACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTa                                                                                                                                                                                                          >  1:345330/1‑250 (MQ=255)                                    ccTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACgg                                                                                                                                                                                                       >  1:298811/1‑250 (MQ=255)                                      ttttCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGa                                                                                                                                                                                                    <  1:145036/251‑1 (MQ=255)                                                cccGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACa                                                                                                                                                                                          <  1:689420/251‑1 (MQ=255)                                                    gTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAAt                                                                                                                                                                                       >  1:676744/1‑250 (MQ=255)                                                    gTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAAt                                                                                                                                                                                       >  1:270355/1‑250 (MQ=255)                                                     tGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATcc                                                                                                                                                                                     <  1:399993/251‑1 (MQ=255)                                                        aCCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGa                                                                                                                                                                                  >  1:559830/1‑251 (MQ=255)                                                          cTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGaaa                                                                                                                                                                                <  1:894098/251‑1 (MQ=255)                                                                gAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCa                                                                                                                                                                          >  1:471211/1‑251 (MQ=255)                                                                     atTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGCTGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCataata                                                                                                                                                                     >  1:828596/1‑251 (MQ=255)                                                                        ttCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTACCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAAcc                                                                                                                                                                  <  1:747242/251‑1 (MQ=255)                                                                            tgttgtCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACg                                                                                                                                                              <  1:825714/251‑1 (MQ=255)                                                                                gtCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAAc                                                                                                                                                                                           <  1:391001/218‑1 (MQ=255)                                                                                gtCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATcc                                                                                                                                                                                     >  1:647368/1‑224 (MQ=255)                                                                                gtCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCtt                                                                                                                                                          >  1:476340/1‑251 (MQ=255)                                                                                     ctTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGAc                                                                                                                                                      >  1:554531/1‑250 (MQ=255)                                                                                             cAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGcc                                                                                                                                             <  1:188873/251‑1 (MQ=255)                                                                                             cAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGc                                                                                                                                              >  1:673723/1‑250 (MQ=255)                                                                                             cAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGc                                                                                                                                              >  1:347327/1‑250 (MQ=255)                                                                                              gaTAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGcc                                                                                                                                             >  1:775947/2‑250 (MQ=255)                                                                                               aTAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCg                                                                                                                                           <  1:408127/251‑1 (MQ=255)                                                                                               aTAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCg                                                                                                                                           <  1:501042/251‑1 (MQ=255)                                                                                                   aaaCGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGagag                                                                                                                                       <  1:73028/251‑1 (MQ=255)                                                                                                        cTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTc                                                                                                                                  <  1:213450/251‑1 (MQ=255)                                                                                                        cTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTc                                                                                                                                  <  1:820238/251‑1 (MQ=255)                                                                                                        cTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTACGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTc                                                                                                                                  <  1:454187/251‑1 (MQ=255)                                                                                                            aaaaaaCCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGa                                                                                                                               <  1:635423/250‑1 (MQ=255)                                                                                                               aaaCCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAAccc                                                                                                                           <  1:736971/251‑1 (MQ=255)                                                                                                                          cTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGc                                                                                                                                                                                                                                       <  1:154934/132‑1 (MQ=255)                                                                                                                          cTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCa                                                                                                                >  1:227294/1‑251 (MQ=255)                                                                                                                                  tttATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGAc                                                                                                        <  1:471885/251‑1 (MQ=255)                                                                                                                                     aTTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAgg                                                                                                     >  1:204105/1‑251 (MQ=255)                                                                                                                                        gatgaGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATgg                                                                                                                                                                                                                                          >  1:506244/1‑115 (MQ=255)                                                                                                                                        gatgaGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGAcc                                                                                                                                                     <  1:297533/200‑1 (MQ=255)                                                                                                                                           gagaAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTa                                                                                               >  1:239239/1‑251 (MQ=255)                                                                                                                                             gaAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTACGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTc                                                                                                                                  <  1:435139/214‑1 (MQ=255)                                                                                                                                              aaGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTaa                                                                                              <  1:524243/249‑1 (MQ=255)                                                                                                                                              aaGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTaa                                                                                              <  1:127421/249‑1 (MQ=255)                                                                                                                                              aaGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAAt                                                                                            <  1:511484/251‑1 (MQ=255)                                                                                                                                                       ggATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGa                                                                                                                                                                                                    <  1:125370/138‑1 (MQ=255)                                                                                                                                                       ggATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAg                                                                                                                                               >  1:656027/1‑191 (MQ=255)                                                                                                                                                       ggATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGt                                                                                   >  1:680535/1‑251 (MQ=255)                                                                                                                                                                  aTGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGagag                                                                                                                                       <  1:232491/188‑1 (MQ=255)                                                                                                                                                                   tGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCt                                                                                                                     <  1:200138/205‑1 (MQ=255)                                                                                                                                                                   tGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTgg                                                                            >  1:785576/1‑246 (MQ=255)                                                                                                                                                                   tGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGgtg                                                                       >  1:857976/1‑251 (MQ=255)                                                                                                                                                                                 gtcgCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGt                                                         >  1:212801/1‑251 (MQ=255)                                                                                                                                                                                     cgTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTcc                                                     <  1:647831/251‑1 (MQ=255)                                                                                                                                                                                           gTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCt                                                                                                                     <  1:625721/181‑1 (MQ=255)                                                                                                                                                                                              gCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGAc                                                                                                        <  1:587478/191‑1 (MQ=255)                                                                                                                                                                                              gCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATa                                            >  1:761397/1‑251 (MQ=255)                                                                                                                                                                                              gCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATa                                            >  1:257110/1‑251 (MQ=255)                                                                                                                                                                                              gCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATa                                            >  1:708377/1‑251 (MQ=255)                                                                                                                                                                                              gCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATa                                            >  1:861067/1‑251 (MQ=255)                                                                                                                                                                                                 tAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATAAAc                                         <  1:718493/251‑1 (MQ=255)                                                                                                                                                                                                       ccTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGc                                                                 >  1:454230/1‑221 (MQ=255)                                                                                                                                                                                                           aaaaTGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATAAACGCCACAAAAg                               <  1:431437/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccGTTTTTTCGTTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATAAACGCCACAAAAGCGGCGGTGCCTAGGCCGACGCCGatatat  <  1:301961/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 TAGCAAATTTATTCATAATAAATTGGGCTTGTGTACCTTTTCCTGCGCCCGGTGCACCTAATAGAATAATTTTCATGTTGTCCTCTTTATGTCAATAAAAACGCTTGAAAAAACCACCGCACTTTTTGGTTTATTGATGAGAAGTGCGGTGGATTTTGAAGATGTTTTAGTAAATCGTCGCGTATGGTTGCCTAATTCCCTGAAAATGTCAATTGTCGGATTTTGCCGCTACCGTTTTTTCATTCCATGGGGCGACCCAAAACAGGCAAAGCATACCCGGTACGGCGAGCAATAAACAAATCCAGAAAAAGTCATAATAACCGACGGCTTTGACCAAATAGCCCGAGAGTATTCCGAACCCTTTGCTTGGCAATGCAGACAGGCTGGTAAATAAGGCGAGTTGGGTGGCGGTGTAAAGCGGGTTAGTTTCCCGTGCCATAAACGCCACAAAAGCGGCGGTGCCTAGGCCGACGCCGATATAT  >  1/896094‑896575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑