Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	891,898	A→G	T196A (ACC→GCC)	unknown →	Hydroxyacylglutathione hydrolase (EC 3.1.2.6)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	891,898	0	A	G	100.0%	186.5 / NA	68	T196A (ACC→GCC)	unknown	Hydroxyacylglutathione hydrolase (EC 3.1.2.6)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

GTATTTAACGGACGGGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCACCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAATATGCCCGTTTTTAATTTTTATTTTTAA  >  1/891654‑892124                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   gTATTTAACGGACGGGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAAt                                                                                                                                                                                                                               >  1:775780/1‑250 (MQ=255) gTATTTAACGGACGGGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAAt                                                                                                                                                                                                                               >  1:40921/1‑250 (MQ=255)   aTTTAACGGACGGGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAg                                                                                                                                                                                                                            >  1:360034/1‑251 (MQ=255)     ttAACGGACGGGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGcc                                                                                                                                                                                                                          <  1:405333/251‑1 (MQ=255)        acggacggGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAg                                                                                                                                                                                                                       >  1:525281/1‑251 (MQ=255)               ggCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCg                                                                                                                                                                                                                >  1:505719/1‑251 (MQ=255)                gCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCGATCAGCCAAGCCTGCCg                                                                                                                                                                                                                <  1:670031/250‑1 (MQ=255)                    ttGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCAc                                                                                                                                                                                                           <  1:375119/251‑1 (MQ=255)                    ttGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCAc                                                                                                                                                                                                           <  1:505362/251‑1 (MQ=255)                                        aTTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACaaa                                                                                                                                                                                       >  1:882326/1‑251 (MQ=255)                                          tttCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAAtt                                                                                                                                                                                     <  1:603969/251‑1 (MQ=255)                                                ccgGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATcc                                                                                                                                                                                <  1:817740/250‑1 (MQ=255)                                                         gACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAAc                                                                                                                                                                       <  1:742762/250‑1 (MQ=255)                                                             ggTATTTACTGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAATACAAATTAATCCGGTTTTAACGGCaa                                                                                                                                                                  >  1:581807/1‑251 (MQ=255)                                                             ggTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCaa                                                                                                                                                                  >  1:25702/1‑251 (MQ=255)                                                                 tttACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAg                                                                                                                                                              <  1:240631/251‑1 (MQ=255)                                                                   tACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTc                                                                                                                                                            <  1:574794/251‑1 (MQ=255)                                                                        gCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCAc                                                                                                                                                                                                           <  1:109754/199‑1 (MQ=255)                                                                         cGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAGACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGgcg                                                                                                                                                       <  1:482482/250‑1 (MQ=255)                                                                            ttACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAAt                                                                                                                                                   >  1:768400/1‑251 (MQ=255)                                                                            ttACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGAGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCAt                                                                                                                                                                                                        >  1:278139/1‑198 (MQ=255)                                                                                 ccaccaTGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACg                                                                                                                                              >  1:260362/1‑251 (MQ=255)                                                                                    ccaTGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCaa                                                                                                                                           >  1:39168/1‑251 (MQ=255)                                                                                        gTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTa                                                                                                                                        >  1:684647/1‑250 (MQ=255)                                                                                          ttGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGc                                                                                                                                     <  1:90801/251‑1 (MQ=255)                                                                                                  aTGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAAc                                                                                                                                                                       >  1:141113/1‑209 (MQ=255)                                                                                                  aTGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCaaa                                                                                                                             >  1:368177/1‑251 (MQ=255)                                                                                                         gTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATc                                                                                                                                                                                                                              >  1:33140/1‑147 (MQ=255)                                                                                                            ttGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCAtttt                                                                                                                   <  1:742146/251‑1 (MQ=255)                                                                                                            ttGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCAtttt                                                                                                                   <  1:216373/251‑1 (MQ=255)                                                                                                                          gACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGcc                                                                                                                                    <  1:130682/220‑1 (MQ=255)                                                                                                                                aCCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCa                                                                                               >  1:496017/1‑251 (MQ=255)                                                                                                                                    tCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATc                                                                                           <  1:372922/251‑1 (MQ=255)                                                                                                                                     cGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCa                                                                                                                                                                                                                         >  1:433014/1‑124 (MQ=255)                                                                                                                                     cGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAAtt                                                                                                                                                                                     >  1:388080/1‑160 (MQ=255)                                                                                                                                     cGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCg                                                                                          >  1:478829/1‑251 (MQ=255)                                                                                                                                     cGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCg                                                                                          >  1:423874/1‑251 (MQ=255)                                                                                                                                       tCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGc                                                                                         <  1:334447/250‑1 (MQ=255)                                                                                                                                        cTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGc                                                                                                                                     >  1:385878/1‑205 (MQ=255)                                                                                                                                          gTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGAt                                                                                     >  1:735189/1‑251 (MQ=255)                                                                                                                                                  cATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGc                                                                              >  1:372755/1‑250 (MQ=255)                                                                                                                                                    tGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCa                                                                                                                               <  1:15171/199‑1 (MQ=255)                                                                                                                                                    tGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGAt                                                                                 <  1:139557/245‑1 (MQ=255)                                                                                                                                                    tGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGctgg                                                                           >  1:550345/1‑248 (MQ=255)                                                                                                                                                    tGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGc                                                                              >  1:142382/1‑248 (MQ=255)                                                                                                                                                    tGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGc                                                                              >  1:839406/1‑248 (MQ=255)                                                                                                                                                        tataCTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGtttt                                                                       <  1:668778/251‑1 (MQ=255)                                                                                                                                                           aCTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGc                                                                                                                                             >  1:809175/1‑178 (MQ=255)                                                                                                                                                           aCTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTAt                                                                    >  1:398613/1‑251 (MQ=255)                                                                                                                                                            cTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAATGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTAtt                                                                   <  1:125198/251‑1 (MQ=255)                                                                                                                                                                aaGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCtt                                                               >  1:276032/1‑251 (MQ=255)                                                                                                                                                                      ccTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAAttt                                                         <  1:493738/251‑1 (MQ=255)                                                                                                                                                                               cGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAg                                                <  1:432328/251‑1 (MQ=255)                                                                                                                                                                                       gCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAg                                                <  1:41167/243‑1 (MQ=255)                                                                                                                                                                                              ggCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAAt                                                                                            <  1:449834/192‑1 (MQ=255)                                                                                                                                                                                               gCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTa                                 <  1:578596/250‑1 (MQ=255)                                                                                                                                                                                                 aaacaaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGaa                              <  1:113630/251‑1 (MQ=255)                                                                                                                                                                                                  aacaaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAat                             <  1:485593/251‑1 (MQ=255)                                                                                                                                                                                                  aacaaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAat                             <  1:243443/251‑1 (MQ=255)                                                                                                                                                                                                   acaaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAata                            <  1:686859/251‑1 (MQ=255)                                                                                                                                                                                                    caaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAg                                                                                                                                                              >  1:303225/1‑120 (MQ=255)                                                                                                                                                                                                    caaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAg                                                <  1:610395/230‑1 (MQ=255)                                                                                                                                                                                                    caaaAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAatat                           >  1:607187/1‑251 (MQ=255)                                                                                                                                                                                                       aaGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAATATGcc                        <  1:207101/251‑1 (MQ=255)                                                                                                                                                                                                              gTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAat                             <  1:482629/239‑1 (MQ=255)                                                                                                                                                                                                              gTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAATATGCCCGttttt                 >  1:503132/1‑251 (MQ=255)                                                                                                                                                                                                                ggAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAATATGCCCGTTTTTaa               >  1:159333/1‑251 (MQ=255)                                                                                                                                                                                                                              ttttGGTGGAACGGCAACGTGCCGCCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGGTAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAATATGCCCGTTTTTAATTTTTATTTTTaa  >  1:427476/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   GTATTTAACGGACGGGCATTTGTTTTGCGGTGACGCGCTATTTTCCGCCGGTTGCGGACGGGTATTTACCGGCGATTACGCCACCATGTTTGCCGCCATGCAACGTTTTGCCAGCTTGCCTGACGAAACCATCGTCTGTCCGGGTCATGAATATACTTTAAGTAACCTGAAATTCGTGGAAAGCGTTTGGGCAAACAAAAGTGCGGTGGAAAATCAGCGCGTTTTGGTGGAACGGCAACGTGCCACCAATCAGCCAAGCCTGCCGAGCACCATCGGCTTGGAAAAACAAATTAATCCGTTTTTAACGGCAACGAGTCTGGCGCAATTTACGCAATTACGCCAGGCAAAAGATCATTTTTAGAAATTTGACAATCTGCAAATCGCAGATTGATAGCCTGTTTTTATTGCTTCAATTTGCTTAAAAGCGGGCAATTCAGCTAGAATATGCCCGTTTTTAATTTTTATTTTTAA  >  1/891654‑892124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑